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Kinetics and mechanisms of cowpea root adaptation to changes in solution calcium

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Abstract

Background and aims

Close regulation of cellular Ca in roots is required in the face of marked changes in soil solution Ca over time and space. This study’s aims were to quantify and gain insights into the ways in which roots respond to changes in solution Ca.

Methods

Root elongation rate (RER) of cowpea (Vigna unguiculata (L.) Walp.) seedlings was determined at 0.05 to 15 mM Ca for up to 24 h both without and with added K, Mg, or Na. Root tip concentrations of Ca, K, Mg, and Na were determined and binding of cations by root tips estimated by subsequent Cu sorption.

Results

Transfer from higher to lower Ca solutions (and with added K at high Ca) resulted in RER ≥ 2 mm h−1 within minutes. This was attributed to greater cell wall relaxation through lower Ca binding aided by a decrease to pH ≤ 5.1 in solution. Transfer to higher Ca solutions, which remained at ~pH 5.6, led to an equally rapid decrease in RER to ~0.5 mm h−1, an effect ascribed to greater cell wall binding of Ca. Thereafter, a gradual increase in RER to ~1.8 mm h−1 occurred over 24 h, an effect likely due to reduced cell wall Ca binding as shown by decreasing Cu sorption at a rate of 0.027 mmol Cu kg−1 FM h−1 over 24 h.

Conclusion

The kinetics of changes in RER and cations in root tips suggest that roots respond to changes in solution Ca through effects on cell wall relaxation of the rhizodermis and outer cortex in the elongation zone.

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Acknowledgements

We thank D. Greenway for advice on statistical analyses. This research was supported in part by an Australian Research Council (ARC) DECRA Award (DE130100943) to Peng Wang and an ARC Future Fellowship (FT120100277) awarded to Peter Kopittke.

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Authors and Affiliations

  1. School of Agriculture and Food Sciences, The University of Queensland, St Lucia, Queensland, Australia, 4072

    F. Pax C. Blamey, J. Bernhard Wehr, Peng Wang, Neal W. Menzies & Peter M. Kopittke

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  1. F. Pax C. Blamey
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  2. J. Bernhard Wehr
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  4. Neal W. Menzies
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  5. Peter M. Kopittke
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Correspondence to F. Pax C. Blamey.

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Responsible Editor: Ismail Cakmak.

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Blamey, F.P.C., Wehr, J.B., Wang, P. et al. Kinetics and mechanisms of cowpea root adaptation to changes in solution calcium. Plant Soil 379, 301–314 (2014). https://doi.org/10.1007/s11104-014-2065-1

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