Plant and Soil

, Volume 375, Issue 1–2, pp 303–315 | Cite as

N- and P-mediated seminal root elongation response in rice seedlings

  • Satoshi Ogawa
  • Michael Gomez Selvaraj
  • Angela Joseph Fernando
  • Mathias Lorieux
  • Manabu Ishitani
  • Susan McCouch
  • Juan David Arbelaez
Regular Article

Abstract

Aims

In rice, seminal root elongation plays an important role in acquisition of nutrients such as N and P, but the extent to which different N forms and P concentrations affect root growth is poorly understood. This study aimed to examine N- and P-mediated seminal root elongation response and to identify putative QTLs associated with seminal root elongation.

Methods

Seminal root elongation was evaluated in 15 diverse wild and cultivated accessions of rice, along with 48 chromosome segment substitution lines (CSSLs) derived from a cross between the rice variety ‘Curinga’ and Oryza rufipogon (IRGC 105491). Root elongation in response to different forms of N (NH4+, NO3 and NH4NO3) and concentrations of P was evaluated under hydroponic conditions, and associated putative QTL regions were identified.

Results

The CSSL parents had contrasting root responses to N and P. Root elongation in O. rufipogon was insensitive to N source and concentration, whereas Curinga was responsive. In contrast to N, seminal root elongation and P concentration was positively correlated. Three putative QTLs for seminal root elongation in response to N were detected on chromosome 1, and one QTL on chromosome 3 was associated with low P concentration.

Conclusions

Genetic variation in seminal root elongation and plasticity of nutrient response may be appropriate targets for marker-assisted selection to improve rice nutrient acquisition efficiency.

Keywords

Chromosome segment substitution lines NH4+ response N acquisition Seminal root elongation 

Abbreviation

CSSL

Chromosome segment substitution line

N

Nitrogen

NAE

Nitrogen acquisition efficiency

P

Phosphorus

PNN

Partial nitrate nutrition

PAE

Phosphorus acquisition efficiency

QTL

Quantitative trait locus

SNP

Single nucleotide polymorphism

SSR

Simple sequence repeat

Supplementary material

11104_2013_1955_MOESM1_ESM.pdf (376 kb)
ESM 1(PDF 375 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Satoshi Ogawa
    • 1
    • 2
  • Michael Gomez Selvaraj
    • 1
  • Angela Joseph Fernando
    • 1
  • Mathias Lorieux
    • 1
    • 3
  • Manabu Ishitani
    • 1
  • Susan McCouch
    • 4
  • Juan David Arbelaez
    • 4
  1. 1.International Center for Tropical Agriculture (CIAT)CaliColombia
  2. 2.Department of Global Agriculture Science, Graduate School of Agriculture and Life ScienceThe University of TokyoTokyoJapan
  3. 3.Institut de Recherche pour le Développement (IRD), DIADE Research UnitMontpellierFrance
  4. 4.Department of Plant Breeding and GeneticsCornell UniversityIthacaUSA

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