Assessment of the relationship between geologic origin of soil, rhizobacterial community composition and soil receptivity to tobacco black root rot in Savoie region (France)
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Background and aims
In Morens (Switzerland), soils formed on morainic deposits (which contain vermiculite clay and display particular tobacco rhizobacterial community) are naturally suppressive to Thielaviopsis basicola-mediated tobacco black root rot, but this paradigm was never assessed elsewhere. Here, we tested the relation between geology and disease suppressiveness in neighboring Savoie (France).
Two morainic and two sandstone soils from Savoie were compared based on disease receptivity (T. basicola inoculation tests on tobacco), clay mineralogy (X-ray diffraction), tobacco rhizobacterial community composition (16S rRNA gene-based taxonomic microarray) and phlD + Pseudomonas populations involved in 2,4-diacetylphloroglucinol production (real-time PCR and tRFLP).
Unlike in Morens, in Savoie the morainic soils were receptive to disease whereas T. basicola inoculation did not increase disease level in the sandstone soils. Vermiculite was not present in Savoie soils. The difference in rhizobacterial community composition between Savoie morainic and sandstone soils was significant but modest, and there was little agreement in bacterial taxa discriminating soils of different disease receptivity levels when comparing Morens versus Savoie soils. Finally, phlD + rhizosphere pseudomonads were present at levels comparable to those in Morens soils, but with different diversity patterns.
The morainic model of black root rot suppressiveness might be restricted to the particular type of moraine occurring in the Morens region, and the low disease receptivity of sandstone soils in neighboring Savoie might be related to other plant-protection mechanisms.
KeywordsSuppressive soil Thielaviopsis basicola Black root rot Moraine Rhizosphere Bacterial community 16S microarray real-time PCR 2,4-diacetylphloroglucinol
This work was supported by CORESTA (Paris, France), the ECO-NET network 10228TF (EGIDE, Paris, France), the Ministry of Education, Youth and Sports of the Czech Republic (project Kontakt ME09077), and the Ministère Français de la Recherche. We are grateful to G. Nicoud (Université de Savoie, Le Bourget du Lac, France) for help with geomorphology and L. Roger (UMR SeqBio, SupAgro, Montpellier, France) with clay analysis. We thank L. Jocteur-Monrozier, J. Haurat (UMR CNRS 5557 Ecologie Microbienne), D. Desbouchage (IFR 41), J. Bernillon (DTAMB) and C. Oger (DTAMB/PRABI) for technical help and/or discussion. This work made use of the IFR41 platforms Serre, DTAMB and PRABI.
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