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Detection of black-foot and Petri disease pathogens in soils of grapevine nurseries and vineyards using bait plants

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Abstract

Background and aims

Little information is currently available regarding the number of species of black-foot and Petri disease pathogens present in soil and their capacity to infect grapevine roots and reach the xylem vessels.

Methods

Seedlings of grapevine rootstock 41-B, and cvs. Bobal and Palomino were planted both in pots containing soil samples collected from commercial vineyards and in nursery fields. Roots and xylem vessels were later analyzed for fungal isolation.

Results

Black-foot pathogens: Ilyonectria alcacerensis, I. macrodidyma, I. novozelandica and I. torresensis were frequently isolated from roots of seedlings grown in all soils evaluated, whereas Petri disease pathogens: Cadophora luteo-olivacea, Phaeoacremonium aleophilum, Pm. parasiticum and Phaeomoniella chlamydospora were only isolated from xylem vessels of seedlings grown in nursery soils, with a low incidence. Ilyonectria alcacerensis, I. novozelandica and I. torresensis were isolated for the first time from grapevines in Spain, and Pm. parasiticum and Ca. luteo-olivacea were detected for the first time in nursery soils.

Conclusions

Our results confirm nursery and vineyard soils as an important inoculum source for black-foot pathogens and demonstrate the presence of several Petri disease pathogens in nursery soils.

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Abbreviations

HIS:

Histone H3 gene

ITS:

Internal transcribed spacer

MEA:

Malt extract agar

MEAS:

Malt extract agar supplemented with 0.5 g l−1 of streptomycin sulphate

PCR:

Polymerase chain reaction

PDA:

Potato dextrose agar

PDAS:

Potato dextrose agar supplemented with 0.5 g l−1 of streptomycin sulphate

TUB:

β-tubulin gene

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Acknowledgments

This research was financially supported by the Project RTA2010-00009-C03-03 (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, INIA, Spain) and the European Regional Development Fund (ERDF). We acknowledge J. M. De la Cruz-Gento, V. Garrigues, P. López-Soriano and V. Palacios for technical assistance, and Dr. B. B. Landa for critically reading the manuscript and making valuable suggestions.

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Authors and Affiliations

  1. Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain

    Carlos Agustí-Brisach, José García-Jiménez & Josep Armengol

  2. Department of Crop Protection, Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Alameda del Obispo s/n, APDO 4084, 14004, Córdoba, Spain

    David Gramaje

Authors
  1. Carlos Agustí-Brisach
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  2. David Gramaje
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  3. José García-Jiménez
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  4. Josep Armengol
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Correspondence to Josep Armengol.

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Responsible Editor: Hans Lambers.

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Agustí-Brisach, C., Gramaje, D., García-Jiménez, J. et al. Detection of black-foot and Petri disease pathogens in soils of grapevine nurseries and vineyards using bait plants. Plant Soil 364, 5–13 (2013). https://doi.org/10.1007/s11104-012-1333-1

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  • DOI: https://doi.org/10.1007/s11104-012-1333-1

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