The efficacy of N,N′-bis(2-hydroxybenzyl)ethylenediamine-N,N′-diacetic acid (HBED) as an Fe source in plant nutrition for soybean (Glycine max) plants grown in calcareous soil under controlled conditions was studied.
The ability of ethylenediamine-N,N′-bis(o-hydroxyphenylacetic) acid (o,oEDDHA/57Fe3+) and HBED/57Fe3+ at increasing concentrations and the long lasting effect were evaluated. In addition, iron nutrition was studied considering the Fe uptake from the chelates prepared with the isotope 57Fe. Further, the SPAD index, total Fe and 57Fe content in plant were analyzed and soluble and available 57Fe fractions in soil were determined over time.
Doses experiment indicated that a higher concentration of HBED/57Fe3+ as compared to o,oEDDHA/57Fe3+ is necessary for obtaining the same 57Fe absorption by the plant; however, these differences were found to be lower in the second sampling time as compared to the first one. This long lasting effect of HBED/Fe3+ was corroborated in the long term experiment. Moreover, it was found that the load of Fe in the pods was higher when using HBED/57Fe3+ than when o,oEDDHA/57Fe3+ was used. The soil extract analysis for each sampling time indicated that HBED/57Fe3+ presented a higher stability in soil than o,oEDDHA/57Fe3+ over time.
The HBED/Fe3+ could be a long lasting alternative to EDDHA/Fe3+ for correcting the iron chlorosis of dicotyledonous plants grown on calcareous soils.
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Ethylenediamine-N-(o-hydroxyphenylacetic acid)-N′-(p-hydroxyphenylacetic acid)
- EDDHA/Fe3+ :
Commercial products based on ferric ethylenediamine-N,N′-bis(o-hydroxyphenylacetate) isomer
Atomic absorption spectroscopy
High-performance liquid chromatography
Inductively coupled plasma mass spectroscopy
Days after treatment
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This research was carried out with the financial support of PPC ADOB (Poland) and the project AGL2010-18048 of the Spanish Ministry of Education and Science. P. Nadal was on a Spanish Ministry of Science and Education “FPI” pre-doctoral grant co-financed by the European Social Fund.
Responsible Editor: Michael A. Grusak.
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Nadal, P., García-Delgado, C., Hernández, D. et al. Evaluation of Fe-N,N′-Bis(2-hydroxybenzyl)ethylenediamine-N,N′-diacetate (HBED/Fe3+) as Fe carrier for soybean (Glycine max) plants grown in calcareous soil. Plant Soil 360, 349–362 (2012). https://doi.org/10.1007/s11104-012-1246-z
- Iron chelates
- Calcareous soil