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Succession of root-associated fungi in Pisum sativum during a plant growth cycle as examined by 454 pyrosequencing

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Abstract

Purpose

Roots are inhabited by a broad range of fungi, including pathogens and mycorrhizal fungi, with functional traits related to plant health and nutrition. Management of these fungi in agroecosystems requires profound knowledge about their ecology. The main objective of this study was to examine succession patterns of root-associated fungi in pea during a full plant growth cycle.

Methods

Plants were grown in pots with field soil in a growth chamber under controlled conditions. Fungal communities in pea roots were analyzed at different plant growth stages including the vegetative growth, flowering and senescence, using 454 pyrosequencing.

Results

One hundred and twenty one non-singleton operational taxonomic units (OTUs) representing fungal species were detected. Pathogenic and arbuscular mycorrhizal fungi dominated during the vegetative growth stage, whereas saprotrophic fungi dominated during plant senescence.

Conclusions

In conclusion, the results from the present study demonstrated highly diverse fungal communities in pea roots with clear succession patterns related to fungal traits.

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Acknowledgements

We thank Karsten Malmskov (Ardo A/S) and Steen Meier for help sampling soil in the field, Anne-Pia Larsen and Jacob I. Bañuelos Trejo for assistance with root staining, Henriette Nyskjold for technical assistance, Bernd Wollenweber for critical review on the manuscript and Kirsten Jensen for linguistic editing.

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Authors and Affiliations

  1. Department of Agroecology, Aarhus University, Research Centre Flakkebjerg, DK-4200, Slagelse, Denmark

    L. Yu, M. Nicolaisen & S. Ravnskov

  2. Centro de Investigaciones en Ecosistemas, Universidad Nacional Autónoma de México, Antigua Carretera a Pátzcuaro 8701, Col. Ex Hacienda de San José de la Huerta, C.P. 58190, Morelia, Michoacán, Mexico

    J. Larsen

Authors
  1. L. Yu
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  2. M. Nicolaisen
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  3. J. Larsen
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  4. S. Ravnskov
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Corresponding author

Correspondence to S. Ravnskov.

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Responsible Editor: Erik J. Joner.

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Yu, L., Nicolaisen, M., Larsen, J. et al. Succession of root-associated fungi in Pisum sativum during a plant growth cycle as examined by 454 pyrosequencing. Plant Soil 358, 225–233 (2012). https://doi.org/10.1007/s11104-012-1188-5

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  • DOI: https://doi.org/10.1007/s11104-012-1188-5

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