Abstract
Both elevated atmospheric carbon dioxide (CO2) and nitrogen (N) deposition may induce changes in C:N ratios in plant tissues and mineral soil. However, the potential mechanisms driving the stoichiometric shifts remain elusive. In this study, we examined the responses of C:N ratios in both plant tissues and mineral soil to elevated CO2 and N deposition using data extracted from 140 peer-reviewed publications. Our results indicated that C:N ratios in both plant tissues and mineral soil exhibited consistent increases under elevated CO2 regimes whereas decreases in C:N ratios were observed in response to experimental N addition. Moreover, soil C:N ratio was less sensitive than plant C:N ratio to both global change scenarios. Our results also showed that the responses of stoichiometric ratios were highly variable among different studies. The changes in C:N ratio did not exhibit strong correlations with C dynamics but were negatively associated with corresponding changes in N content. These results suggest that N dynamics drive stoichiometric shifts in both plant tissues and mineral soil under both elevated CO2 and N deposition scenarios.
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References
Billes G, Rouhier H, Bottner P (1993) Modifications of the carbon and nitrogen allocations in the plant (Triticum aestivum L.) soil system in response to increased atmospheric CO2 concentration. Plant Soil 157:215–225
Chapin FS, Matson PA, Mooney HA (2002) Principles of terrestrial ecosystem ecology. Springer, New York
Cleveland CC, Liptzin D (2007) C:N:P stoichiometry in soil: is there a “Redfield ratio” for the microbial biomass? Biogeochemistry 85:235–252
Cotrufo MF, Ineson P, Scott A (1998) Elevated CO2 reduces the nitrogen concentration of plant tissues. Glob Chang Biol 4:43–54
Cui Q, Lü X, Wang Q, Han X (2010) Nitrogen fertilization and fire act independently on foliar stoichiometry in a temperate steppe. Plant Soil 334:209–219
Elser JJ, Bracken MES, Cleland EE, Gruner DS, Harpole WS, Hillebrand H, Ngai JT, Seabloom EW, Shurin JB, Smith JE (2007) Global analysis of nitrogen and phosphorus limitation of primary production in freshwater, marine, and terrestrial ecosystems. Ecol Lett 10:1135–1142
Elser JJ, Kyle M, Steger L, Nydick KR, Baron JS (2009a) Nutrient availability and phytoplankton nutrient limitation across a gradient of atmospheric nitrogen deposition. Ecology 90:3062–3073
Elser JJ, Andersen T, Baron JS, Bergstrom A, Jansson M, Kyle M, Nydick KR, Steger L, Hessen DO (2009b) Shifts in lake N:P stoichiometry and nutrient limitation driven by atmospheric nitrogen deposition. Science 326:835–837
Elser JJ, Fagan WF, Kerkhoff AJ, Swenson NG, Enquist BJ (2010) Biological stoichiometry of plant production: metabolism, scaling and ecological response to global change. New Phytol 186:593–608
Emmett BA, Brittain SA, Hughes S, Kennedy V (1995) Nitrogen additions (NaNO3 and NH4NO3) at Aber forest, Wales: II. Response of trees and soil nitrogen transformations. For Ecol Manag 71:61–73
Esmeijer-Liu AJ, Aerts R, Kürschner WM, Bobbink R, Lotter AF, Verhoeven JTA (2009) Nitrogen enrichment lowers Betula pendula green and yellow leaf stoichiometry irrespective of effects of elevated carbon dioxide. Plant Soil 316:311–322
Forster P et al (2007) Changes in atmospheric constituents and in radiative forcing. In: Solomon S, Qin D, Manning M, Chen Z, Marquis M, Averyt KB, Tignor M, Miller HL (eds) Climate Change 2007: The Physical Science Basis. Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge
Gifford RM, Barrett DJ, Lutze JL (2000) The effects of elevated CO2 on the C:N and C:P mass ratios of plant tissues. Plant Soil 224:1–14
Gruber N, Galloway JN (2008) An earth-system perspective of the global nitrogen cycle. Nature 451:293–296
Güsewell S (2004) N:P ratios in terrestrial plants: variation and functional significance. New Phytol 164:243–266
He J-S, Fang J, Wang Z, Guo D, Flynn DFB, Geng Z (2006) Stoichiometry and large-scale patterns of leaf carbon and nitrogen in the grassland biomes of China. Oecologia 149:115–122
Hedges LV, Gurevitch J, Curtis PS (1999) The meta-analysis of response ratios in experimental ecology. Ecology 80:1150–1156
Heimann M, Reichstein M (2008) Terrestrial ecosystem carbon dynamics and climate feedbacks. Nature 451:289–292
Johnson D, Leake JR, Lee JA (1999) The effects of quantity and duration of simulated pollutant nitrogen deposition on root-surface phosphatase activities in calcareous and acid grasslands: a bioassay approach. New Phytol 141:433–442
Knops JMH, Naeem S, Reich PB (2007) The impact of elevated CO2, increased nitrogen availability and biodiversity on plant tissue quality and decomposition. Glob Chang Biol 13:1960–1971
LeBauer DS, Treseder KK (2008) Nitrogen limitation of net primary productivity in terrestrial ecosystems is globally distributed. Ecology 89:371–379
Lu M, Yang YH, Luo YQ, Fang CM, Zhou XH, Chen JK, Yang X, Li B (2010a) Responses of ecosystem nitrogen cycle to nitrogen addition: a meta-analysis. New Phytol 189:1040–1050
Lu M, Zhou XH, Luo YQ, Yang YH, Fang CM, Chen JK, Li B (2010b) Minor stimulation of soil carbon storage by nitrogen addition: a meta-analysis. Agric Ecosyst Environ 140:234–244
Luo Y et al (2004) Progressive nitrogen limitation of ecosystem responses to rising atmospheric carbon dioxide. Bioscience 54:731–739
Luo Y, Hui DF, Zhang DQ (2006a) Elevated CO2 stimulates net accumulations of carbon and nitrogen in land ecosystems: a meta-analysis. Ecology 87:53–63
Luo Z, Calfapertra C, Liberloo M, Scarascia-mugnozza G, Polle A (2006b) Carbon partitioning to mobile and structural fractions in poplar wood under elevated CO2 (EUROFACE) and N fertilization. Glob Chang Biol 12:272–283
Manzoni S, Trofymow JA, Jackson RB, Porporato A (2010) Stoichiometric controls on carbon, nitrogen, and phosphorus dynamics in decomposing litter. Ecol Monogr 80:89–106
McGroddy ME, Daufresne T, Hedin LO (2004) Scaling of C:N:P stoichiometry in forests worldwide: implications of terrestrial redfield-type ratios. Ecology 85:2390–2401
McGuire AD, Mellio JM, Joyce LA (1995) The role of nitrogen in the response of forest net primary production to elevated atmospheric carbon-dioxide. Ann Rev Ecolog Syst 26:473–503
Norby RJ, Cotrufo MF, Ineson P, O’Neill EG, Canadell JG (2001) Elevated CO2, litter chemistry, and decomposition: a synthesis. Oecologia 127:153–165
Novotny AM, Schade JD, Hobbie SE, Kay AD, Kyle M, Reich PB, Elser JJ (2007) Stoichiometric response of nitrogen-fixing and non-fixing dicots to manipulations of CO2, nitrogen, and diversity. Oecologia 151:687–696
Poorter H et al (1997) The effect of elevated CO2 on the chemical composition and construction costs of leaves of 27 C3 species. Plant Cell Environ 20:472–482
R Development Core Team (2010) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna
Reich PB, Oleksyn J (2004) Global patterns of plant leaf N and P in relation to temperature and latitude. Proc Natl Acad Sci USA 101:11001–11006
Reich PB et al (2001) Do species and functional groups differ in acquisition and use of C, N and water under varying atmospheric CO2 and N availability regimes? A field test with 16 grassland species. New Phytol 150:435–448
Reich PB, Oleksyn J, Wright IJ (2009) Leaf phosphorus influences the photosynthesis-nitrogen relation: a cross-biome analysis of 314 species. Oecologia 160:207–212
Sterner RW, Elser JJ (2002) Ecological stoichiometry: the biology of elements from molecules to the biosphere. Princeton University Press, New Jersey
Sterner RW, Anagnostou E, Brovold S, Bullerjahn GS, Finlay JC, Kumar S, McKay RML, Sherrell RM (2007) Increasing stoichiometric imbalance in North America’s largest lake: nitrification in Lake Superior. Geophys Res Lett 34:L10406. doi:10.1029/2006GL028861
Strain BR, Bazzaz FA (1983) Terrestrial Plant Communities. In: Lemon ER (ed) CO2 and plants. The response of plants to rising levels of atmospheric carbon dioxide. Westview, Boulder
Thayer SS, St Clair SB, Field CB, Somerville SC (2008) Accentuation of phosphorus limitation in Geranium dissectum by nitrogen: an ecological genomics study. Glob Chang Biol 14:1877–1890
Vitousek PM, Howarth RW (1991) Nitrogen limitation on land and in the sea—how can it occur? Biogeochemistry 13:87–115
Vitousek PM, Porder S, Houlton BZ, Chadwick OA (2010) Terrestrial phosphorus limitation: mechanisms, implications, and nitrogen-phosphorus interactions. Ecol Appl 20:5–15
Wedin DA, Tilman D (1996) Influence of nitrogen loading and species composition on the carbon balance of grasslands. Science 274:1720–1723
Yang YH, Luo Y (2010) Carbon: nitrogen stoichiometry during forest stand development. Glob Ecol Biogeogr 20:354–361
Yang YH, Fang JY, Guo DL, Ji CJ, Ma WH (2010) Vertical patterns of soil carbon, nitrogen and carbon: nitrogen stoichiometry in Tibetan grasslands. Biogeosciences Discuss 7:1–24
Yuan ZY, Li LH, Han XG, Chen SP, Wang ZW, Chen QS, Bai WM (2006) Nitrogen response efficiency increased monotonically with decreasing soil resource availability: a case study from a semiarid grassland in northern China. Oecologia 148:564–572
Acknowledgements
We are grateful to Dr. Klaus Butterbach-Bahl and two anonymous reviewers for their insightful comments on earlier drafts of the manuscript. We also appreciate Joshua Kalfas for assisting with English grammar. We thank all scientists whose data and work are included in this data synthesis. This study was financially supported by the US National Science Foundation (NSF) under DEB 0743778, DEB 0840964, DBI 0850290, and EPS 0919466; by the Office of Science (BER), Department of Energy, Grants No.: DE-FG02-006ER64319 and through the Midwestern Regional Center of the National Institute for Climatic Change Research at Michigan Technological University, under Award Number DE-FC02-06ER64158. WXH was supported by the National Natural Science of China (40973054).
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Responsible Editor: Klaus Butterbach-Bahl.
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Appendix S1
A list of papers from which data are extracted for this synthesis. (DOC 73 kb)
Appendix S2
Dataset of carbon content, nitrogen content, and carbon: nitrogen ratio in shoot, root and mineral soil under control and treatment conditions, together with reference sources, ecosystem type and experimental treatment. (XLS 171 kb)
Table S1
Terrestrial C:N ratio dynamics in response to elevated CO2 (+ CO2), N addition (+ N), and both elevated CO2 and N addition (+ CO2 + N). Values are means ± SE. The sample sizes (n) refers to the total number of the analyzed data points for each ecosystem component. (DOC 33 kb)
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Yang, Y., Luo, Y., Lu, M. et al. Terrestrial C:N stoichiometry in response to elevated CO2 and N addition: a synthesis of two meta-analyses. Plant Soil 343, 393–400 (2011). https://doi.org/10.1007/s11104-011-0736-8
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DOI: https://doi.org/10.1007/s11104-011-0736-8