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Diverse rhizobia associated with soybean grown in the subtropical and tropical regions of China

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Abstract

Aiming at investigating the species composition and the association between ribosomal/housekeeping genes and symbiotic genes of rhizobia nodulating with soybean grown in the subtropical and tropic regions of China, a total of 252 rhizobial strains isolated from five eco-regions was characterized. Four genomic groups, Bradyrhizobium japonicum complex (including B. liaoningense, B. japonicum and a B. japonicum related genomic species) and B. elkanii as the major groups, B. yuanmingense and Sinorhizobium fredii as the minor groups, were identified by the ribosomal/housekeeping gene analyses. The symbiotic gene phylogenies were coherent with those of the housekeeping genes in these four genomic groups, indicating that the symbiotic genes were mainly maintained by vertical transfer in the soybean rhizobia. In correspondence analysis, the Bradyrhizobium species were not significantly related to the eco-regions, possibly due to the similar climate and soil conditions in these regions.

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Acknowledgements

This work was financial supported by the foundation of the State Key Basic Research and Development Plan of China (grant 2006CB100206), National Natural Science Foundation of China (grant 30670001), and by National Project for Basic S&T Platform Construction (grant 2005DKA21201-10). ETW was supported by the project of SIP 20070538 and 20080322.

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Authors and Affiliations

  1. Key laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, Beijing, 100094, China

    Chao Xin Man, Hao Wang, Wen Feng Chen, Xin Hua Sui & Wen Xin Chen

  2. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico, D. F., Mexico

    En Tao Wang

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  1. Chao Xin Man
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  2. Hao Wang
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  3. Wen Feng Chen
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Correspondence to Wen Xin Chen.

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Responsible Editor: Euan K. James.

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Man, C.X., Wang, H., Chen, W.F. et al. Diverse rhizobia associated with soybean grown in the subtropical and tropical regions of China. Plant Soil 310, 77–87 (2008). https://doi.org/10.1007/s11104-008-9631-3

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