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Growth, nitrogen fixation and ammonium assimilation in common bean (Phaseolus vulgaris L) subjected to iron deficiency

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Abstract

A greenhouse experiment was carried out aiming to study the effect of iron deficiency on nitrogen fixation and ammonium assimilation in common bean nodules. Host-plant and nodule growth, symbiotic nitrogen fixation, glutamine synthetase (GS) and glutamate dehydrogenase (GDH) were analyzed in two common bean varieties subjected to iron deficiency. Results showed that host-plant and nodules growth, nitrogen fixation and GS activity decreased when under Fe-deficiency against an important increase of ammonium accumulation and GDH activity. Tolerant variety Flamingo is clearly less affected by iron deficiency than the sensitive one, Coco blanc. The allocation of iron to nodules and Fe use-efficiency for nodule growth and symbiotic nitrogen fixation were on the basis of the symbiotic performance of Flamingo under iron deprivation. Under Fe-deficiency, GDH take over GS the ammonium assimilation activity, particularly in the tolerant variety.

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Abbreviations

ARA:

acetylene reduction activity

GDH:

glutamate dehydrogenase

GOGAT:

glutamate synthase

GS:

glutamine synthetase

SNF:

symbiotic nitrogen fixation

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Acknowledgements

This work was supported by grants from the Tunisian Ministry of Higher Education, Scientific Research and the Technology.

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Authors and Affiliations

  1. Laboratoire d’Adaptation des Plantes aux Stress Abiotiques, Centre de Biotechnologie, Technopark de Borj Cedria, BP 901, Hammam Lif, Tunis, 2050, Tunisia

    Tarek Slatni, Abdelmajid Krouma, Samir Aydi & Chedly Abdelly

  2. Unité de Nutrition et Métabolisme Azotés et Protéines de Stress, Département de Biologie, Faculté des Sciences de Tunis, Campus Universitaire, 1060, Tunis, Tunisia

    Tarek Slatni, Chiraz Chaiffi & Houda Gouia

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  1. Tarek Slatni
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  2. Abdelmajid Krouma
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  3. Samir Aydi
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  4. Chiraz Chaiffi
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  5. Houda Gouia
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  6. Chedly Abdelly
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Correspondence to Chedly Abdelly.

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Slatni, T., Krouma, A., Aydi, S. et al. Growth, nitrogen fixation and ammonium assimilation in common bean (Phaseolus vulgaris L) subjected to iron deficiency. Plant Soil 312, 49–57 (2008). https://doi.org/10.1007/s11104-007-9481-4

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  • DOI: https://doi.org/10.1007/s11104-007-9481-4

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