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Characteristics of amino acid uptake in barley

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Abstract

Plants have the ability to take up organic nitrogen (N) but this has not been thoroughly studied in agricultural plants. A critical question is whether agricultural plants can acquire amino acids in a soil ecosystem. The aim of this study was to characterize amino acid uptake capacity in barley (Hordeum vulgare L.) from a mixture of amino acids at concentrations relevant to field conditions. Amino acids in soil solution under barley were collected in microlysimeters. The recorded amino acid composition, 0–8.2 μM of l-Serine, l-Glutamic acid, Glycine, l-Arginine and l-Alanine, was then used as a template for uptake studies in hydroponically grown barley plants. Amino acid uptake during 2 h was studied at initial concentrations of 2–25 μM amino acids and recorded as amino acid disappearance from the incubation solution, analysed with HPLC. The uptake was verified in control experiments using several other techniques. Uptake of all five amino acids occurred at 2 μM and below. The concentration dependency of the uptake rate could be described by Michaelis–Menten kinetics. The affinity constant (K m) was in the range 19.6–33.2 μM. These K m values are comparable to reported values for soil micro-organisms.

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Abbreviations

DM:

Dry matter

HPLC:

High-performance liquid chromatography

References

  • Epstein E (1961) The essential role of calcium in selective cation transport by plant cells. Plant Physiol 36:437–444

    Article  PubMed  CAS  Google Scholar 

  • Falkengren-Grerup U, Månsson KF, Olsson MO (2000) Uptake capacity of amino acids by ten grasses and forbs in relation to soil acidity and nitrogen availability. Environ Exp Bot 44:207–219

    Article  PubMed  CAS  Google Scholar 

  • Hirner A, Ladwig F, Stransky H, Okumoto S, Keinath M, Harms A, Frommer WB, Koch W (2006) Arabidopsis LHT1 is a high-affinity transporter for cellular amino acid uptake in both root epidermis and leaf mesophyll. Plant Cell 18:1931–1946

    Article  PubMed  CAS  Google Scholar 

  • Hutchinson HB, Miller NHJ (1911) The direct assimilation of inorganic and organic forms of nitrogen by higher plants. Centbl Bakt II 30:513–547

    Google Scholar 

  • Jones DL (1999) Amino acid biodegradation and its potential effects on organic nitrogen capture by plants. Soil Biol Biochem 31:613–622

    Article  CAS  Google Scholar 

  • Jones DL, Darrah PR (1994) Amino-acid influx at the soil–root interface of Zea mays L and its implications in the rhizosphere. Plant Soil 163:1–12

    CAS  Google Scholar 

  • Jones DL, Hodge A (1999) Biodegradation kinetics and sorption reactions of three differently charged amino acids in soil and their effects on plant organic nitrogen availability. Soil Biol Biochem 31:1331–1342

    Article  CAS  Google Scholar 

  • Jones DL, Kielland K (2002) Soil amino acid turnover dominates the nitrogen flux in permafrost-dominated taiga forest soils. Soil Biol Biochem 34:209–219

    Article  CAS  Google Scholar 

  • Jones DL, Shannon D, Junvee-Fortune T, Farrarc JF (2005) Plant capture of free amino acids is maximized under high soil amino acid concentrations. Soil Biol Biochem 37:179–181

    Article  CAS  Google Scholar 

  • Kielland K (1994) Amino-acid-absorption by arctic plants – implications for plant nutrition and nitrogen cycling. Ecology 75:2373–2383

    Article  Google Scholar 

  • Lee YH, Foster J, Chen J, Voll LM, Weber APM, Tegeder M (2007) AAP1 transports uncharged amino acids into roots of Arabidopsis. Plant J 50:305–319

    Article  PubMed  CAS  Google Scholar 

  • Lipson DA, Raab TK, Schmidt SK, Monson RK (1999) Variation in competitive abilities of plants and microbes for specific amino acids. Biol Fertil Soils 29:257–261

    Article  CAS  Google Scholar 

  • Lipson DA, Raab TK, Schmidt SK, Monson RK (2001) An empirical model of amino acid transformations in an alpine soil. Soil Biol Biochem 33:189–198

    Article  CAS  Google Scholar 

  • Melin E, Nilsson H (1953) Transfer of labelled nitrogen from L-Glutamic acid to pine seedlings through the mycelium of Boletus variegatus (Sw.) Fr. Nature 171:134

    Article  PubMed  CAS  Google Scholar 

  • Miller AE, Bowman WD (2003) Alpine plants show species-level differences in the uptake of organic and inorganic nitrogen. Plant Soil 250:283–292

    Article  CAS  Google Scholar 

  • Miller RH, Schmidt EL (1965) Uptake and assimilation of amino acids supplied to the sterile soil: root environment of the bean plant (Phaseolus vulgaris). Soil Sci 100:323–330

    Article  CAS  Google Scholar 

  • Näsholm T, Sandberg G, Ericsson A (1987) Quantitative-analysis of amino-acids in conifer tissues by high-performance liquid-chromatography and fluorescence detection of their 9-fluorenylmethyl chloroformate derivatives. J Chromatogr 396:225–236

    Article  Google Scholar 

  • Näsholm T, Ekblad A, Nordin A, Giesler R, Högberg M, Högberg P (1998) Boreal forest plants take up organic nitrogen. Nature 392:914–916

    Article  Google Scholar 

  • Näsholm T, Huss-Danell K, Högberg P (2000) Uptake of organic nitrogen in the field by four agriculturally important plant species. Ecology 81:1155–1161

    Google Scholar 

  • Näsholm T, Huss-Danell K, Högberg P (2001) Uptake of glycine by field grown wheat. New Phytol 150:59–63

    Article  Google Scholar 

  • Ohlsson KEA, Wallmark PH (1999) Novel calibration with correction for drift and non-linear response for continuous flow isotope ratio mass spectrometry applied to the determination of delta N-15, total nitrogen, delta C-13 and total carbon in biological material. Analyst 124:571–577

    Article  Google Scholar 

  • Öhlund J (2004) Organic and inorganic nitrogen sources for conifer seedlings: abundance, uptake and growth. Doctoral thesis. Swedish University of Agricultural Sciences. Acta Universitatis Agriculturae Sueciae Silvestria 312

  • Owen AG, Jones DL (2001) Competition for amino acids between wheat roots and rhizosphere micro-organisms and the role of amino acids in plant N acquisition. Soil Biol Biochem 33:651–657

    Article  CAS  Google Scholar 

  • Paynel F, Cliquet JB (2003) N transfer from white clover to perennial rye grass, via exudation of nitrogenous compounds. Agronomie 23:503–510

    Article  CAS  Google Scholar 

  • Paynel F, Murray PJ, Cliquet JB (2001) Root exudates: a pathway for short-term N transfer from clover and ryegrass. Plant Soil 229:235–243

    Article  CAS  Google Scholar 

  • Persson J, Näsholm T (2001) Amino acid uptake: a widespread ability among boreal forest plants. Ecol Lett 4:434–438

    Article  Google Scholar 

  • Phillips DA, Fox TC, King MD, Bhuvaneswari TV, Teuber LR (2004) Microbial products trigger amino acid exudation from plant roots. Plant Physiol 136:2887–2894

    Article  PubMed  CAS  Google Scholar 

  • Raab TK, Lipson DA, Monson RK (1996) Non-mycorrhizal uptake of amino acids by roots of the alpine sedge Kobresia myosuroides: implications for the alpine nitrogen cycle. Oecologia 108:488–494

    Article  Google Scholar 

  • Raab TK, Lipson DA, Monson RK (1999) Soil amino acid utilization among species of the Cyperaceae: plant soil processes. Ecology 80:2408–2419

    Article  Google Scholar 

  • Schimel JP, Bennett J (2004) Nitrogen mineralization: challenges of a changing paradigm. Ecology 85:591–602

    Article  Google Scholar 

  • Schimel JP, Chapin FS (1996) Tundra plant uptake of amino acid and NH4+ nitrogen in situ: plants compete well for amino acid N. Ecology 77:2142–2147

    Article  Google Scholar 

  • Schmidt S, Stewart GR (1999) Glycine metabolism by plant roots and its occurrence in Australian plant communities. Aust J Plant Physiol 26:253–264

    Article  CAS  Google Scholar 

  • Schobert C, Komor E (1987) Amino-acid-uptake by Ricinus communis roots – characterization and physiological significance. Plant Cell Environ 10:493–500

    Article  CAS  Google Scholar 

  • Soldal T, Nissen P (1978) Multiphasic uptake of amino acids by barley roots. Physiol Plant 43:181–188

    Article  CAS  Google Scholar 

  • Svennerstam H, Ganeteg U, Bellini C, Näsholm T (2007) Comprehensive screening of Arabidopsis mutants suggests the lysine histidine transporter 1 to be involved in plant uptake of amino acids. Plant Physiol 143:1853–1860

    Article  PubMed  CAS  Google Scholar 

  • Vinolas LC, Healey JR, Jones DL (2001) Kinetics of soil microbial uptake of free amino acids. Biol Fertil Soils 33:67–74

    Article  CAS  Google Scholar 

  • Virtanen AI, Linkola H (1946) Organic nitrogen compounds as nitrogen nutrition for higher plants. Nature 157:515

    Google Scholar 

  • Wright DE (1962) Amino acid uptake by plant roots. Arch Biochem Biophys 97:174–180

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

We would like to thank Margareta Zetherström for support and technical assistance in the laboratory and Bo Ranneby and Jun Yu for statistical advice. Svalöf-Weibull AB kindly provided the barley seeds. This study was financially supported by the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning.

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Correspondence to Sandra Jämtgård.

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Responsible Editor: A.C. Borstlap.

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Jämtgård, S., Näsholm, T. & Huss-Danell, K. Characteristics of amino acid uptake in barley. Plant Soil 302, 221–231 (2008). https://doi.org/10.1007/s11104-007-9473-4

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