Characteristics of amino acid uptake in barley
- 497 Downloads
Plants have the ability to take up organic nitrogen (N) but this has not been thoroughly studied in agricultural plants. A critical question is whether agricultural plants can acquire amino acids in a soil ecosystem. The aim of this study was to characterize amino acid uptake capacity in barley (Hordeum vulgare L.) from a mixture of amino acids at concentrations relevant to field conditions. Amino acids in soil solution under barley were collected in microlysimeters. The recorded amino acid composition, 0–8.2 μM of l-Serine, l-Glutamic acid, Glycine, l-Arginine and l-Alanine, was then used as a template for uptake studies in hydroponically grown barley plants. Amino acid uptake during 2 h was studied at initial concentrations of 2–25 μM amino acids and recorded as amino acid disappearance from the incubation solution, analysed with HPLC. The uptake was verified in control experiments using several other techniques. Uptake of all five amino acids occurred at 2 μM and below. The concentration dependency of the uptake rate could be described by Michaelis–Menten kinetics. The affinity constant (K m) was in the range 19.6–33.2 μM. These K m values are comparable to reported values for soil micro-organisms.
KeywordsActive uptake Affinity constant Amino acids in soil solution Efflux Nitrogen acquisition Organic nitrogen
High-performance liquid chromatography
We would like to thank Margareta Zetherström for support and technical assistance in the laboratory and Bo Ranneby and Jun Yu for statistical advice. Svalöf-Weibull AB kindly provided the barley seeds. This study was financially supported by the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning.
- Hutchinson HB, Miller NHJ (1911) The direct assimilation of inorganic and organic forms of nitrogen by higher plants. Centbl Bakt II 30:513–547Google Scholar
- Jones DL, Darrah PR (1994) Amino-acid influx at the soil–root interface of Zea mays L and its implications in the rhizosphere. Plant Soil 163:1–12Google Scholar
- Näsholm T, Huss-Danell K, Högberg P (2000) Uptake of organic nitrogen in the field by four agriculturally important plant species. Ecology 81:1155–1161Google Scholar
- Ohlsson KEA, Wallmark PH (1999) Novel calibration with correction for drift and non-linear response for continuous flow isotope ratio mass spectrometry applied to the determination of delta N-15, total nitrogen, delta C-13 and total carbon in biological material. Analyst 124:571–577CrossRefGoogle Scholar
- Öhlund J (2004) Organic and inorganic nitrogen sources for conifer seedlings: abundance, uptake and growth. Doctoral thesis. Swedish University of Agricultural Sciences. Acta Universitatis Agriculturae Sueciae Silvestria 312Google Scholar
- Virtanen AI, Linkola H (1946) Organic nitrogen compounds as nitrogen nutrition for higher plants. Nature 157:515Google Scholar