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Heat shock-mediated H2O2 accumulation and protection against Cd toxicity in rice seedlings

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Abstract

Rice (Oryza sativa L.) seedlings stressed with CdCl2 (0.5 mM or 50 μM) showed typical Cd toxicity (leaf chlorosis, decrease in chlorophyll content, or increase in H2O2 and malondialdehyde contents). Rice seedlings pretreated with heat shock at 45°C (HS) for 2 or 3 h were protected against subsequent Cd stress. Rice seedlings pretreated with HS had similar Cd concentration in leaves caused by CdCl2 as those non-HS. The content of H2O2 increased in leaves 1 h after HS exposure. However, APX and GR activities were higher in HS-treated leaves than their respective control, and it occurred after 2 h of HS treatment. Pretreatment of rice seedlings with H2O2 under non-HS conditions resulted in an increase in APX, GR, and CAT activities and protected rice seedlings from subsequent Cd stress. HS-induced H2O2 production and protection against subsequent Cd stress can be counteracted by imidazole, an inhibitor of NADPH oxidase complex. Results of the present study suggest that early accumulation of H2O2 during HS signals the increase in APX and GR activities, which in turn prevents rice seedlings from Cd-caused oxidative damage.

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Abbreviations

APX:

Ascorbate peroxidase

ASC:

Ascorbate

CAT:

Catalase

DAB:

3, 3-Diaminobenzidine

DW:

Dry weight

GR:

Glutathione reductase

HS:

Heat shock

IMD:

Imidazole

MDA:

Malondialdehyde

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgements

This work was supported by a research grant the National Science Council of the Republic of China (NSC 95-2313-B-002-046).

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  1. Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

    Yi Ting Hsu & Ching Huei Kao

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  1. Yi Ting Hsu
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  2. Ching Huei Kao
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Correspondence to Ching Huei Kao.

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Responsible Editor: Juan Barcelo.

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Hsu, Y.T., Kao, C.H. Heat shock-mediated H2O2 accumulation and protection against Cd toxicity in rice seedlings. Plant Soil 300, 137–147 (2007). https://doi.org/10.1007/s11104-007-9396-0

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