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Toxic effects of Pb2+ on the growth and mineral nutrition of signal grass (Brachiaria decumbens) and Rhodes grass (Chloris gayana)

Abstract

Although grasses are commonly used to revegetate sites contaminated with lead (Pb), little is known regarding the Pb-tolerance of many of these species. Using dilute solution culture to mimic the soil solution, the growth of signal grass (Brachiaria decumbens Stapf cv. Basilisk) and Rhodes grass (Chloris gayana Kunth cv. Pioneer) was related to the mean activity of Pb2+ {Pb2+} in solution. There was a 50% reduction in fresh mass of signal grass shoots at 5 μM {Pb2+} and at 3 μM {Pb2+} for the roots. Rhodes grass was considerably more sensitive to Pb in solution, with shoot and root fresh mass being reduced by 50% at 0.5 μM {Pb2+}. The higher tolerance of signal grass to Pb appeared to result from the internal detoxification of Pb, rather than from the exclusion of Pb from the root. At toxic {Pb2+}, an interveinal chlorosis developed in the shoots of signal grass (possibly a Pb-induced Mn deficiency), whilst in Rhodes grass, Pb2+ caused a bending of the root tips and the formation of a swelling immediately behind some of the root apices. Root hair growth did not appear to be reduced by Pb2+ in solution, being prolific at all {Pb2+} in both species.

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Abbreviations

AMF:

Arbuscular mycorrhizal fungi

DAP:

Days after planting

EC:

Electrical conductivity

FIA:

Flow injection analysis

ICP-MS/OES:

Inductively coupled plasma-mass spectrometry/optical emission spectrometry

XPS:

X-ray photoelectron spectroscopy

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Acknowledgments

The authors thank Rosemary Kopittke for statistical assistance and Associate Professor Stephen Adkins for the use of the dissecting microscope. This research was funded through the Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC-CARE) Project 3-3-01-05/6.

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Correspondence to P. M. Kopittke.

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Responsible Editor: Henk Schat.

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Kopittke, P.M., Asher, C.J., Blamey, F.P.C. et al. Toxic effects of Pb2+ on the growth and mineral nutrition of signal grass (Brachiaria decumbens) and Rhodes grass (Chloris gayana). Plant Soil 300, 127–136 (2007). https://doi.org/10.1007/s11104-007-9395-1

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  • DOI: https://doi.org/10.1007/s11104-007-9395-1

Keywords

  • Root and shoot growth
  • Root hairs
  • Chlorosis
  • Tolerance