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The participation of the cell wall hydrolytic enzymes in the initial colonization of Azospirillum brasilense on wheat roots

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Abstract

The effect of cellulase and pectinase on bacterial colonization of wheat was studied by three different experiments. In the first experiment, the root colonization of 3 wheat cultivars (Ghods, Roshan and Omid) by two A. brasilense strains (Sp7 and Dol) was compared using pre-treated roots with cellulase and pectinase, and non-treated with these enzymes (control). Although the root colonization varied greatly among strain-plant combinations in controls, the pre-treatment of roots with polysaccharide degrading enzymes significantly increased the bacterial count in roots, regardless of the strain-plant combination. This might be an indication that cell wall may act as an important factor in plant-Azospirillum interaction. In the second experiment, the root cellulase activity of the same wheat cultivars treated with and without the two Azospirillum brasilense, strains (Sp7 and Dol) was compared. The pre-treatment of wheat roots with Azospirillum enhanced the cellulase activity of wheat root extracts. Thus, the cellulase activity might participate in the initial colonization of wheat roots by Azospirillum. The comparison of the cellulase activity of root extracts within inoculated and non-inoculated seedlings showed that the inoculation had enhanced the cellulase activity in root extracts, but this effect was directly dependent on the strain-plant combination. Strain Sp7 stimulated the highest cellulase activity in cv. Roshan, but strain Dol induced the highest enzyme activity in cv. Ghods. In the third experiment, several growth parameters of those 3 wheat cultivars treated with and without those two bacterial strains (Sp7 and Dol) were compared. The highest magnitude of growth responses caused by Sp7 strain was in the cv Roshan, but Dol strain stimulated the highest growth in cv Ghods. Therefore, effective colonization may contribute to more growth responses.

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References

  • Al-Mallah MK, Davey MR, Cocking EC (1987) Enzymatic treatment of clover root hairs removes a barrier to Rhizobium-host specificity. Nat Biotechnol 5:1319–1322

    Article  CAS  Google Scholar 

  • Al-Mallah MK, Davey MR, Cocking EC (1989) Formation of nodular structures on rice seedlings by rhizobia. J Exp Bot 40:473–478

    Article  Google Scholar 

  • Amooaghaie R (2001) Investigation of interaction between A. brasilense and root cells of wheat in initial stages of root inoculation. PhD Thesis, University of Isfahan, Isfahan, Iran

  • Amooaghaie R, Mosatjeran A, Emtiazi G (2002b) The effect of compatible and incompatible Azospirillum brasilense strains on proton efflux of intact wheat roots. Plant Soil 243:155–160

    Article  CAS  Google Scholar 

  • Amooaghaie R, Mosatjeran A, Emtiazi G (2002a) The effect of strain and concentration of Azospirillum brasilense bacterium on growth and development of root in wheat cultivars. Iranian J Agricultural Sci 33:213–222

    Google Scholar 

  • Assmus B, Hutzler P, Kirchhof G, Amann R, lawrence JR, Hartmann A (1995) In situ localization of Azospirillum brasilense in the rhizosphere of wheat with fluorescently labelled, rRNA-targeted oligonucleotide probes and scanning confocal laser microscopy. Appl Environ Microbiol 61:1013–1019

    PubMed  CAS  Google Scholar 

  • Baldani VLD, Baldani JI, Doebereiner J (1983) Effects of Azospirillum inoculation on root infection and nitrogen incorporation in wheat. Can J Microbiol 29:924–929

    Google Scholar 

  • Bashan Y (1986) Enhancement of wheat root colonization and plant development by Azospirillum brasilense Cd. following temporary depression of the rhizosphere microflora. Appl Environ Microbiol 51:1067–1071

    PubMed  CAS  Google Scholar 

  • Bashan Y, Holguin G (1993) Anchoring of Azospirillum brasilense to hydrophobic polystyrene and wheat roots. J Gen Microbiol 139:379–385

    CAS  Google Scholar 

  • Bashan Y, Holguin G (1997) Azospirillum plant relationships: environmental and physiological advances (1990–1996). Can J Microbiol 43:103–121

    Article  CAS  Google Scholar 

  • Bashan Y, Levanony H (1989) Factors affecting adsorption of Azospirillum brasilense Cd to root hairs as compared with root surface of wheat. Can J Microbiol 35:936–944

    Article  CAS  Google Scholar 

  • Bashan Y, Levanony H (1985) An improved selection technique and medium for the isolation and enumeration of Azospirillum brasilense. Can J Microbiol 31:947–952

    CAS  Google Scholar 

  • Bhattarai T, Hess D (1993) Yield responses of Nepalese spring wheat (T. aestivum) cultivars to inoculation with Azospirillum spp. of Nepalese origin. Plant Soil 151:67–76

    Article  CAS  Google Scholar 

  • Day JM, Dobereiner J (1976) Physiological aspects of N2-fixation by a spirillum from digitaria roots. Soil Biol Biochem 12:433–439

    Google Scholar 

  • Dazzo FB, Napoli CA, Hubbell DH (1976) Adsorption of bacteria to roots as related to host specificity in the Rhizobium–clover symbiosis. Appl Environ Microbiol 32:166–171

    PubMed  CAS  Google Scholar 

  • Del Gallo M, Negi M, Neyra CA (1989) Calcofluor and lectin-binding exocellular polysaccharides of A. brasilense and A. lipoferum. J Bacteriol 171:3504–3510

    PubMed  Google Scholar 

  • Gafny R, Okon Y, Kapulnik Y, Fischer M (1986) Adsorption of Azospirillum brasilense to corn roots. Soil Biol Biochem 18:69–75

    Article  CAS  Google Scholar 

  • Garcia de Salomone I, Dobereiner J (1996) Maize genotype effects on the response to Azospirillum inoculation. Biol Fertil Soil 21:193–196

    Google Scholar 

  • Hoagland DR, Arnon DL (1950) The water-culture method of growing plants without soil. Calif Agric Exp Stn 374:1–32

    Google Scholar 

  • Hubbell DH (1981) Legume infection by Rhizobium: a conceptual approach. BioScience 31:832–837

    Article  Google Scholar 

  • Jain DK, Patriquin G (1984) Root hair deformation, bacterial attachment, and plant growth in wheat-Azospirillum associations. Appl Environ Microbiol 48:1208–1213

    PubMed  CAS  Google Scholar 

  • Kapulnik Y, Okon Y, Heins Y (1985) Changes in root morphology of wheat caused by Azospirillum inoculation. Can J Microbiol 31:881–887

    Google Scholar 

  • Khammas KM, Kaiser P (1991) Characterization of a pectinolytic activity in Azospirillum irakense. Plant Soil 137:75–79

    Article  CAS  Google Scholar 

  • Levanony H, Bashan Y, Romono B, Klein E (1989) Ultrastructural localization and identification of A. brasilense Cd on and within wheat root by immono-gold labelling. Plant Soil 117:207–218

    Article  Google Scholar 

  • Mandel M, Weber J (1969) Exoglucanase activities of microorganisms. Adv Chem 95:391–414

    Article  Google Scholar 

  • Mateos PF, Jimenez-Zurdo JI, Chen L, Squartini AS, Haack SK, Martinez-Molina E, Hubbell DH, Dazzo FB (1992) Cell-associated pectinolytic and cellulolytic enzymes in Rhizobium leguminosarum biovar trifolii. Appl Environ Microbiol 58:1816–1822

    PubMed  CAS  Google Scholar 

  • Morales VM, Monila EM, Hubbell DH (1984) Cellulase production by Rhizobium. Plant Soil 80:407–415

    Article  CAS  Google Scholar 

  • Mostajeran A, Amooaghaie R, Emtiazi G (2002) Root hair density and deformation of inoculated roots of wheat cultivars by A. brasilense and role of IAA in this phenomenon. Iranian Biol J 13:18–28

    Google Scholar 

  • Myers ML, Hubbell DH (1987) Plant cell wall carbohydrates as substrates for Azospirillum brasilense. Appl Environ Microbiol 53:2745–2748

    PubMed  CAS  Google Scholar 

  • Okon Y (1985) Azospirillum as a potential inoculant for Agriculture. Trends Biotechnol 3:223–228

    Article  Google Scholar 

  • Okon Y, Kapulnik Y (1986) Development and function of Azospirillum-inoculated roots. Plant Soil 90:3–16

    Article  CAS  Google Scholar 

  • Patriquin DG, Döbereiner J, Jain DX (1983) Sites and processes of association between diazotrophs and grasses. Can J Microbiol 29:900–915

    Article  Google Scholar 

  • Plazinski J, Rolfe BG (1985) Analysis of the pectolytic activity of Rhizobium and Azospirillum strains isolated from Trifolium repens. J Plant Physiol 120:181–187

    CAS  Google Scholar 

  • Puente ME, Bashan Y (1993) Effect of inoculation with Azospirillum brasilense strains on the germination and seedling growth of the giant columnar cardon cactus (Parchycereus pringlei). Symbiosis 15:49–60

    Google Scholar 

  • Rao PSK, Arnuachalam V, Tilak K (1990) Genotype dependent response to Azospirillum treatment in yield and nitrogenase activity in Brassica juncea. Curr Sci 59:605–609

    Google Scholar 

  • Reinhold-Hurek B, Hureck T, Claeyssens M, Van-Montasu M (1993) Cloning expression in Escherchia Coli and Characterization of cellulolytic enzyrnes of Azoarcus sp. J Bacteriol 175:7056–7065

    PubMed  CAS  Google Scholar 

  • Reinhold B, Hureck T (1988) Location of diazotrophs in the root interior with special attention to the kallar grass association. Plant Soil 110:259 268

    Article  Google Scholar 

  • Rothballer M, Schmid M, Hartmann A (2003) In situ localization and PGPR-effect of Azospirillum brasilense strains colonizing roots of different wheat varieties. Symbiosis 34:261–279

    Google Scholar 

  • Schloter M, Hartmann A (1998) Endophytic and surface colonization of wheat roots (Triticum aestivum) by different Azospirillum brasilense strains studied with strain-specific monoclonal antibodies. Symbiosis 25:159–179

    Google Scholar 

  • Tien TM, Diem HG, Gaskins MH, Hubbell DH (1981) Polygalacturonic acid transeliminase production by Azospirillum species. Can J Microbiol 27:426–431

    PubMed  CAS  Google Scholar 

  • Umali-Garcia M, Hubbell DH, Gashins MH, Dazzo FB (1980) Association of Azospirillum with grass roots. Appl Environ Microbiol 39:219–226

    PubMed  CAS  Google Scholar 

Download references

Acknowledgment

This work was supported by research grant of University of Esfahan in Iran.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran

    A. Mostajeran & G. Emtiazi

  2. Department of Biology, Faculty of Science, University of Shahrekord, Shaherkord, Iran

    R. Amooaghaie

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  1. A. Mostajeran
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  2. R. Amooaghaie
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  3. G. Emtiazi
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Correspondence to G. Emtiazi.

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Mostajeran, A., Amooaghaie, R. & Emtiazi, G. The participation of the cell wall hydrolytic enzymes in the initial colonization of Azospirillum brasilense on wheat roots. Plant Soil 291, 239–248 (2007). https://doi.org/10.1007/s11104-006-9189-x

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  • DOI: https://doi.org/10.1007/s11104-006-9189-x

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