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Mechanical Impedance and Nutrient Acquisition in Rice

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Abstract

The aim of this work was to examine the effect of mechanical impedance on nutrient acquisition and gene expression in rice (Oryza sativa L.). Roots were mechanically impeded in a sand-core apparatus to vary impedance independently of aeration and water status. The effect of impedance on plant growth, anion concentration and expression of genes for anion transporters was compared for six varieties with differences in root penetration ability. Impedance decreased shoot growth more than root growth in all varieties, resulting in increased root/shoot ratios. Impedance substantially increased shoot tissue nitrate concentration in all varieties but only caused a small increase in shoot sulphate and phosphate concentrations. High impedance increased expression of the sulphate transporter OsST1 in five varieties, which was associated with decreased sulphate concentration in root tissues. In contrast, impedance decreased expression of the phosphate transporter OsPT2 expression in all varieties, which was associated with decreased phosphate concentration in root tissues. Localisation of expression of the sulphate transporter by in situ hybridisation indicated high levels of expression in lateral bud primordia. It was suggested that the decreased root phosphate concentrations of impeded roots were caused by low phosphate transporter gene expression, while the increase in sulphate transporter gene expression was due to a derepression mechanism of control.

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References

  • B J Atwell (1988) ArticleTitlePhysiological responses of lupin roots to soil compaction Plant Soil 111 277–281 Occurrence Handle10.1007/BF02139953

    Article  Google Scholar 

  • B J Atwell (1990) ArticleTitleThe effect of soil compaction on wheat during early tillering. II. Concentrations of cell constituents New Phytol. 115 37–41

    Google Scholar 

  • P B Barraclough A H Weir (1988) ArticleTitleEffects of a compacted subsoil layer on root and shoot growth, water use and nutrient uptake of winter wheat J. Agr. Sci., Cambridge 110 207–216

    Google Scholar 

  • A G Bengough C E Mullins (1990) ArticleTitleMechanical impedance to root growth: A review of experimental techniques and root growth responses J. Soil Sci. 41 341–358

    Google Scholar 

  • L J Clark S L Aphalé P B Barraclough (2000) ArticleTitleScreening the ability of rice roots to overcome the mechanical impedance of wax layers: Importance of test conditions and measurement criteria Plant Soil 219 187–196 Occurrence Handle10.1023/A:1004753900945 Occurrence Handle1:CAS:528:DC%2BD3cXjsVWltrk%3D

    Article  CAS  Google Scholar 

  • L J Clark R E Cope W R Whalley P B Barraclough L J Wade (2002) ArticleTitleRoot penetration of strong soil in rainfed lowland rice: Comparison of laboratory screens with field performance Field Crops Res. 76 189–198 Occurrence Handle10.1016/S0378-4290(02)00039-4

    Article  Google Scholar 

  • C Croser A G Bengough J Pritchard (1999) ArticleTitleThe effect of mechanical impedance on root growth in pea (Pisum sativum). I. Rates of cell flux, mitosis, and strain during recovery Physiol. Plant. 107 277–286 Occurrence Handle10.1034/j.1399-3054.1999.100304.x Occurrence Handle1:CAS:528:DC%2BD3cXlt1ensQ%3D%3D

    Article  CAS  Google Scholar 

  • C Gotor F J Cejudo C Barroso J M Vega (1997) ArticleTitleTissue-specific expression of ATCYS-3A, a gene encoding the cytosolic isoform of O-acetylserine(thiol)lyase in Arabidopsis Plant J. 11 347–352 Occurrence Handle10.1046/j.1365-313X.1997.11020347.x Occurrence Handle1:CAS:528:DyaK2sXitFCkur4%3D Occurrence Handle9076998

    Article  CAS  PubMed  Google Scholar 

  • M J Hawkesford (2003) ArticleTitleTransporter gene families in plants: The sulphate transporter gene family – redundancy or speciali- zation? Physiol. Plant. 117 155–165 Occurrence Handle10.1034/j.1399-3054.2003.00034.x Occurrence Handle1:CAS:528:DC%2BD3sXitlKrsLk%3D

    Article  CAS  Google Scholar 

  • M J Hawkesford J L Wray (2000) ArticleTitleMolecular genetics of sulphate assimilation Adv. Bot. Res. 33 159–223 Occurrence Handle1:CAS:528:DC%2BD3cXmvFyju7w%3D

    CAS  Google Scholar 

  • Y Huang W R Jordan R A Wing P W Morgan (1998) ArticleTitleGene expression induced by physical impedance in maize roots Plant Mol. Biol. 37 921–930 Occurrence Handle10.1023/A:1006034411529 Occurrence Handle1:CAS:528:DyaK1cXkvF2ltLc%3D Occurrence Handle9700065

    Article  CAS  PubMed  Google Scholar 

  • M Iijima Y Kono (1991) ArticleTitleInterspecific differences of the root system structures of four cereal species as affected by soil compaction Jpn. J. Crop Sci. 60 130–138

    Google Scholar 

  • D K Kundu J K Ladha E Lapitan de Guzman (1996) ArticleTitleTillage depth influence on soil nitrogen distribution and availability in a rice lowland Soil Sci. Soc. Am. J. 60 1153–1159 Occurrence Handle1:CAS:528:DyaK28Xktl2qsbw%3D

    CAS  Google Scholar 

  • J Masle J B Passioura (1987) ArticleTitleThe effect of soil strength on the growth of young wheat plants Aust. J. Plant Physiol. 14 643–656

    Google Scholar 

  • S A Materechera A R Dexter A M Alston (1991) ArticleTitlePenetration of very strong soils by seedling roots of different plant species Plant Soil 135 31–41 Occurrence Handle10.1007/BF00014776

    Article  Google Scholar 

  • S R Mudge A L Rae E Diatloff F W Smith (2002) ArticleTitleExpression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis Plant J. 31 341–353 Occurrence Handle10.1046/j.1365-313X.2002.01356.x Occurrence Handle1:CAS:528:DC%2BD38XntFelsb8%3D Occurrence Handle12164813

    Article  CAS  PubMed  Google Scholar 

  • R W Payne (2000) A Guide to GenStat Release 8. Part 2 – Statistics VSN International Oxford 997

    Google Scholar 

  • A H Price K A Steele B J Moore P B Barraclough L J Clark (2000) ArticleTitleA combined RFLP and AFLP linkage map of upland rice (Oryza sativa L.) used to identify QTLs for root-penetration ability Theor. Appl. Genet. 100 49–56 Occurrence Handle10.1007/s001220050007 Occurrence Handle1:CAS:528:DC%2BD3cXht1aqtrw%3D

    Article  CAS  Google Scholar 

  • J Sambrook E F Fritsch T Maniatis (1989) Molecular Cloning: A Laboratory Manual EditionNumber2nd edition Cold Spring Harbor Laboratory Press Cold Spring Harbor

    Google Scholar 

  • B K Samson M Hasan L J Wade (2002) ArticleTitlePenetration of hardpans by rice lines in the rainfed lowlands Field Crops Res. 76 175–188 Occurrence Handle10.1016/S0378-4290(02)00038-2

    Article  Google Scholar 

  • F W Smith M J Hawkesford P M Ealing D T Clarkson P J Vanden Berg A R Belcher A G S Warrilow (1997) ArticleTitleRegulation of expression of a cDNA from barley roots encoding a high affinity sulphate transporter Plant J. 12 875–884 Occurrence Handle1:CAS:528:DyaK2sXns1yitrY%3D Occurrence Handle9375399

    CAS  PubMed  Google Scholar 

  • M Ternesi A P Andrade J Jorrin M Benlloch (1994) ArticleTitleRoot–shoot signalling in sunflower plants with confined root systems Plant Soil 166 31–36 Occurrence Handle10.1007/BF02185478 Occurrence Handle1:CAS:528:DyaK2MXjsFKmurY%3D

    Article  CAS  Google Scholar 

  • T C Verwoerd B M M Dekker A Hoekema (1989) ArticleTitleA small-scale procedure for the rapid isolation of plant RNAs Nucleic Acids Res. 17 2362 Occurrence Handle1:CAS:528:DyaL1MXitVyrurs%3D Occurrence Handle2468132

    CAS  PubMed  Google Scholar 

  • L J Wade S Fukai B K Samson A Ali M A Mazid (1999) ArticleTitleRainfed lowland rice: Physical environment and cultivar requirements Field Crops Res. 64 3–12

    Google Scholar 

  • Yoshida S and Hasegawa S 1982 The rice root system: Its development and function. In Drought Resistance in Crops with Emphasis on Rice. pp. 97–114. IRRI, Los Baños, Philippines

  • L Yu J D Ray J C O’Toole H T Nguyen (1995) ArticleTitleUse of wax–petrolatum layers for screening rice root penetration Crop Sci. 35 684–687

    Google Scholar 

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Author notes

  1. Donna A. Brown

    Present address: The Physiological Society, P.O. Box 11319, London, WC1X 8WQ, UK

Authors and Affiliations

  1. Crop Performance and Improvement Division, Rothamsted Research, AL5 2JQ, Harpenden, Hertfordshire, UK

    Donna A. Brown, Jonathan R. Howarth, Saroj Parmar & Malcolm J. Hawkesford

  2. Agriculture and Environment Division, Rothamsted Research, AL5 2JQ, Harpenden, Hertfordshire, UK

    Lawrence J. Clark

Authors
  1. Donna A. Brown
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  2. Lawrence J. Clark
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  4. Saroj Parmar
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  5. Malcolm J. Hawkesford
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Correspondence to Lawrence J. Clark.

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Brown, D.A., Clark, L.J., Howarth, J.R. et al. Mechanical Impedance and Nutrient Acquisition in Rice. Plant Soil 280, 65–76 (2006). https://doi.org/10.1007/s11104-005-2508-9

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  • DOI: https://doi.org/10.1007/s11104-005-2508-9

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