Abstract
Seedlings of Eucalyptus regnans (mountain ash) grow poorly in undried forest soil, where they develop purple coloration in the foliage, but their growth is markedly improved when forest soil has been air dried. Whether this growth promotion is purely due to improved nutrient status of the soil, as a result of air drying, was investigated. In several pot experiments, E. regnans seedlings were grown (i) in air-dried and undried forest soil with addition of different levels of complete fertiliser, (ii) in air-dried or undried soil diluted to different extents with sand, or (iii) in undried soil mixed with different amounts of air-dried soil. Seedling dry weight, P content and incidence of ectomycorrhizal root tips were determined.
In all experiments, the dry weights of seedlings were 3–6 times greater in 100% air-dried soil than in 100% undried soil. Fertiliser application resulted in a significant increase in dry weight of seedlings in both air-dried and undried soil, but the dry weights in air-dried soil were always significantly greater than those in undried soil at the same level of fertiliser application. Even at the highest level of fertiliser application, the growth difference between seedlings in air-dried and undried soil remained. When air-dried soil was diluted with sand, there was a significant reduction in seedling dry weight only when soil was diluted to 20% or less (air-dried soil:total mix). Conversly, when air-dried soil was mixed with undried soil, there was a proportional decrease in seedling dry weight with increasing amounts of undried soil. In all experiments, the dominant ectomycorrhizal morphotypes in 100% air-dried soil were different from those in undried soil. Fertilisation and dilution of air-dried and undried soil did not result in a reduction in the overall incidence of ectomycorrhizal root tips, although the frequency of occurrence of different ectomycorrhizal morphotypes was affected.
It is concluded that the growth difference between seedlings in air-dried and undried forest soils is not due solely to differences in the direct availability of nutrients in the soils, and different ectomycorrhizae may indirectly affect nutrient availability to the plant.
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References
D H Ashton (1976) ArticleTitleStudies on the Mycorrhizae of Eucalyptus regnans F. Muell. Aust. J. Bot 24 723–741
D H Ashton P M Attiwill (1994) Tall open forests R H Groves (Eds) Australian Vegetation EditionNumber2 Cambridge University Press Cambridge 157–196
D H Ashton K J Kelliher (1996a) ArticleTitleEffects of forest soil desiccation on the growth of Eucalyptus regnans F. Muell. Seedlings. Veg. Sci 7 487–496
D H Ashton K J Kelliher (1996) ArticleTitleThe effect of soil desiccation on the nutrient status of Eucalyptus regnans F. Muell. seedlings. Plant Soil 179 45–56 Occurrence Handle1:CAS:528:DyaK28XisFelsb4%3D
D H Ashton J S Turner (1979) ArticleTitleStudies on the compensation point of Eucalyptus regnans F. Muell. Aust. J. Bot 27 589–607
D H Ashton E J Willis (1982) Antagonism in the regeneration of Eucalyptus regnans in the mature forest E I Newman (Eds) he Plant Community as a Working Mechanism Blackwell Scientific Publications Oxford 113–128
P R Beckjord J H Melhuish SuffixJr M S McIntosh (1985) ArticleTitleEffects of nitrogen and phosphorus fertilisation on growth and formation of ectomycorrhizae of Quercus alba and Q. rubra seedlings by Pisolithus tinctorus and Scleroderma auranteum Can. J. Bot 63 1677–1680
C Brule P Frey-Klett J C Pierrat S Courrier F Gerard M C Lemoine J L Rousselet G Sommer J Garbaye (2001) ArticleTitleSurvival in the soil of the ectomycorrhizal fungus Laccaria bicolor and the effects of a mycorrhiza helper Pseudomonas fluorescens Soil Biol. Biochem 33 1683–1694 Occurrence Handle1:CAS:528:DC%2BD3MXotFOlurw%3D
I Brunner C Scheidegger (1994) ArticleTitleEffects of high nitrogen concentration on ectomycorrhizal structure and growth of seedlings of Picea abies (L.) Karst New Phytol 129 83–95
T I Burgess N Malajczuk T S Grove (1993) ArticleTitleThe ability of 16 ectomycorrhizal fungi to increase growth and phosphorus uptake of Eucalyptus globulus Labill. and E. diversicolor F. Muell. Plant Soil 153 155–164 Occurrence Handle1:CAS:528:DyaK2cXltlOrsA%3D%3D
D P Chambers P M Attiwill (1994) ArticleTitleThe ash-bed effect in Eucalyptus regnans forest: Chemical, physical and microbiological changes in soil after heating or partial sterilisation Aust. J. Bot 42 739–749 Occurrence Handle1:CAS:528:DyaK2MXktFKntbc%3D
T Domisch L Finer T Lehto A Smolander (2002) ArticleTitleEffect of soil temperature on nutrient allocation and mycorrhizas in Scots pine seedlings Plant and Soil 239 173–185 Occurrence Handle1:CAS:528:DC%2BD38XktFWisb0%3D
R G Florence R L Crocker (1963) ArticleTitleAnalysis of blackbutt (Eucalyptus pilularis Sm.) seedling growth in a blackbutt forest soil Ecology 43 670–679
Grove T S, Malajczuk N, Burgess T, Thomson B D and Hardy G 1991. Growth responses of plantations eucalypts to inoculation with selected ectomycorrhizal fungi. In Proceedings IUFRO Symposium ‘Intensive Forestry: the Role of Eucalypts’, pp. 86–93.
A Guerin-Laguette C Plassard D Mousain (2000) ArticleTitleEffects of experimental conditions on mycorrhizal relationships between Pinus sylvestris and Lactarius deliciosus and unprecedented fruit-body formation of the Saffron milk cap under controlled soilless conditions Can. J. Microbiol 46 790–799 Occurrence Handle1:CAS:528:DC%2BD3cXmvVGlu7o%3D Occurrence Handle11006839
J L Harley (1989) ArticleTitleThe significance of mycorrhiza Mycol. Res 92 129–139
P A Heinrich J W Patrick (1986) ArticleTitlePhosphorus acquisition in the soil-root system of Eucalyptus pilularis Sm. Seedlings. II The effect of ectomycorrhizas on seedling phosphorus and dry weight acquisition Aust. J. Bot 34 445–454
P A Heinrich D R Mulligan J W Patrick (1988) ArticleTitleThe effect of ectomycorrhizas on the phosphorus and dry weight acquisition of Eucalyptus seedlings Plant Soil 109 147–149
J Katan (1981) ArticleTitleSolar heating (solarization) of soil for control of soil borne pests Ann. Rev. Phytopath 19 211–236
R E Koske J N Gemma (1989) ArticleTitleA modified procedure for staining roots to detect VA mycorrhizas Mycol. Res 92 486–505
T L Launonen D H Ashton P J Keane (1999) ArticleTitleThe effect of regeneration burns on the growth, nutrient acquisition and mycorrhizae of Eucalyptus regnans F. Muell. (mountain ash) seedlings Plant Soil 210 273–283
Launonen T L, Ashton D H and Keane P J The growth and P acquisition of Eucalyptus regnans F. Muell. seedlings in air dried and undried forest soil in relation to seedling age and ectomycorrhizal infection. Plant Soil (in press).
N Malajczuk A J McComb J F Loneragan (1975) ArticleTitlePhosphorus uptake and growth of mycorrhizal and uninfected seedlings of Eucalyptus calophylla R.Br Aust. J. Bot 23 231–238
H Marschner B Dell (1994) ArticleTitleNutrient uptake in mycorrhizal symbiosis Plant Soil 159 89–102 Occurrence Handle1:CAS:528:DyaK2cXis1yqu78%3D
K J Mullette N J Hannon A G L Elliott (1974) ArticleTitleInsoluble phosphorus usage by Eucalyptus Plant Soil 41 194–205
Y Piche J A Fortin (1982) ArticleTitleDevelopment of mycorrhizae, extramatrical mycelium and sclerotia on Pinus strobus seedlings New Phytol 91 211–220
B D Thomson A D Robson L K Abbott (1991) ArticleTitleSoil mediated effects of phosphorus supply on the formation of mycorrhizas by Scutellispora calospora (Nicol. & Gerd.) Walker & Sanders on subterranean clover New Phytol 118 463–469 Occurrence Handle1:CAS:528:DyaK3MXmtlWhsL8%3D
J H Warcup (1981) Effect of fire on the soil microflora and other non-vascular plants A M Gill R H Groves I R Noble (Eds) Fire and the Australian Biota Australian Academy of Science Canberra 208–214
Warcup JH 1983. Effect of fire and sun-baking on the soil microflora and seedling growth in forest soils. In Soils: An Australian Viewpoint. pp.~735–740. Academic Press, London.
J H Warcup (1991) ArticleTitleThe fungi forming mycorrhizas on eucalypt seedlings in regeneration coupes in Tasmania Mycol. Res 95 329–332
Willis E J 1980. Allelopathy and its role in forests of Eucalyptus regnans F.Muell. Ph.D. Thesis, University of Melbourne.
J H Zar (1984) Biostatistical Analysis Prentice-Hall New Jersey USA 718
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Launonen, T.M., Ashton, D.H. & Keane, P.J. Growth, nutrient acquisition and ectomycorrhizae of Eucalyptus regnans F. Muell. seedlings in fertilized or diluted air-dried and undried forest soil. Plant Soil 268, 221–231 (2005). https://doi.org/10.1007/s11104-004-0279-3
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DOI: https://doi.org/10.1007/s11104-004-0279-3