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miR397-LACs mediated cadmium stress tolerance in Arabidopsis thaliana

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Abstract

Cadmium (Cd) is a non-essential heavy metal, assimilated in plant tissue with other nutrients, disturbing the ions’ homeostasis in plants. The plant develops different mechanisms to tolerate the hazardous environmental effects of Cd. Recently studies found different miRNAs that are involved in Cd stress. In the current study, miR397 mutant lines were constructed to explore the molecular mechanisms of miR397 underlying Cd tolerance. Compared with the genetically modified line of overexpressed miR397 (artificial miR397, amiR397), the lines of downregulated miR397 (Short Tandem Target Mimic miR397, STTM miR397) showed more substantial Cd tolerance with higher chlorophyll a & b, carotenoid and lignin content. ICP-OES revealed higher cell wall Cd and low total Cd levels in STTM miR397 than in the wild-type and amiR397 plants.

Further, the STTM plants produced fewer reactive oxygen species (ROS) and lower activity of antioxidants enzymes (e.g., catalase [CAT], malondialdehyde [MDA]) compared with amiR397 and wild-type plants after stress, indicating that silencing the expression of miR397 can reduce oxidative damage. In addition, the different family transporters’ gene expression was much higher in the amiR397 plants than in the wild type and STTM miRNA397. Our results suggest that miR397 plays a role in Cd tolerance in Arabidopsis thaliana. Overexpression of miR397 could decrease Cd tolerance in plants by regulating the expression of LAC 2/4/17, changing the lignin content, which may play an important role in inducing different stress-tolerant mechanisms and protecting the cell from a hazardous condition. This study provides a basis to elucidate the functions of miR397 and the Cd stress tolerance mechanism in Arabidopsis thaliana.

Key message

The miR397 modified lines influence the lignin and Cd content in the plants. The amiR397 plants susceptible to Cd stress have less lignin and high Cd content than STTM miR397 plants, changing the underlying stress regulatory pathways.

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Funding

This work was supported by Shenzhen Natural Science Fund (the Stable Support Plan Program, 20220804115333001), Natural Science Foundation of Guangdong province (2020A1515010309), Chinese National Key R & D Project for Synthetic Biology (2018YFA0902500), National Natural Science Foundation of China (32273118), Guangdong Key R & D Project (2022B1111070005), Shenzhen Special Fund for Sustainable Development (KCXFZ20211020164013021) and Shenzhen University 2035 Initiative to Dr. Zhangli Hu.

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  1. Shahid Ali and Shili Huang have contributed equally to this work.

Authors and Affiliations

  1. Guangdong Provincial Key Laboratory for Plant Epigenetics; Shenzhen Key Laboratory of Marine Bioresource & Eco-Environmental Sciences, Longhua Institute of Innovative Biotechnology, College of Life Science and Oceanography, Shenzhen University, Shenzhen, 518060, Guangdong Province, China

    Shahid Ali, Shili Huang, Jiajie Zhou, Yongsheng Bai, Liyu Shi, Zhangli Hu & Yulin Tang

  2. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, Guangdong Province, China

    Shahid Ali

  3. Guangdong Academy of Forestry, Guangzhou, 510520, Guangdong Province, China

    Yang Liu

  4. Shaanxi Academy of Traditional Chinese Medicine, Xi’an, 710003, Shaanxi, China

    Shuai Liu

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SA, SH, and YT designed, analyzed, and prepared the manuscript. SA, SH, and JZ assisted with experiments. YB, YL, LS, SL, and ZH evaluated data and revised the manuscript. All authors contributed to the manuscript and approved the submitted version.

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Ali, S., Huang, S., Zhou, J. et al. miR397-LACs mediated cadmium stress tolerance in Arabidopsis thaliana. Plant Mol Biol 113, 415–430 (2023). https://doi.org/10.1007/s11103-023-01369-x

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