Abstract
Long noncoding RNAs (lncRNAs) participate in plant biological processes under biotic and abiotic stresses. However, little is known about the function and regulation mechanism of lncRNAs related to the pathogen at a molecular level. A banana lncRNA, Malnc2310, is a Fusarium oxysporum f. sp. cubense inducible lncRNA in roots. In this study, we demonstrate the nuclear localization of Malnc2310 by fluorescence in situ hybridization and it can bind to several proteins that are related to flavonoid pathway, pathogen response and programmed cell death. Overexpression of Malnc2310 increases susceptibility to Fusarium crude extract (Fu), salinity, and cold in transgenic Arabidopsis. In addition, Malnc2310 transgenic Arabidopsis accumulated more anthocyanins under Fusarium crude extract and cold treatments that are related to upregulation of these genes involved in anthocyanin biosynthesis. Based on our findings, we propose that Malnc2310 may participate in flavonoid metabolism in plants under stress. Furthermore, phenylalanine ammonia lyase (PAL) protein expression was enhanced in Malnc2310 overexpressed transgenic Arabidopsis, and Malnc2310 may participate in PAL regulation by binding to it. This study provides new insights into the role of Malnc2310 in mediating plant stress adaptation.
Key message
The lncRNA Malnc2310 is induced in banana roots by Fusarium. The 35S::Malnc2310 transgenic Arabidopsis accumulate more anthocyanin under stress, verifying that Malnc2310 involved in flavonoid pathway through regulating PAL proteins.
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This study was supported by the High-Level Talents Project of Hainan Provincial Natural Science Foundation of China (2019RC292) and the National Key Research and Development Program of China (2019YFD1000204).
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This study was supported by High-Level Talents Project of Hainan Provincial Natural Science Foundation (Grant No. 2019RC292) and the National Key Research and Development Program (Grant No. 2019YFD1000204).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by WL, PZ, JS, XY, LZ, and SL. The first draft of the manuscript was written by WL. The manuscript was revised by RD, MR and MP. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Li, W., Zhao, P., Sun, J. et al. Biological function research of Fusarium oxysporum f. sp. cubense inducible banana long noncoding RNA Malnc2310 in Arabidopsis. Plant Mol Biol 112, 293–307 (2023). https://doi.org/10.1007/s11103-023-01360-6
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DOI: https://doi.org/10.1007/s11103-023-01360-6