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Supportive role of the Na+ transporter CmHKT1;1 from Cucumis melo in transgenic Arabidopsis salt tolerance through improved K+/Na+ balance

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Abstract

Key message

CmHKT1;1 selectively exports Na+ from plant cells. Upon NaCl stress, its expression increased in a salt-tolerant melon cultivar. Overexpression of CmHKT1;1 increased transgenic Arabidopsis salt tolerance through improved K+/Na+ balance.

Abstract

High-affinity K+ transporters (HKTs) are thought to be involved in reducing Na+ in plant shoots under salt stress and modulating salt tolerance, but their function in a moderately salt-tolerant species of melon (Cucumis melo L.) remains unclear. In this study, a Na+ transporter gene, CmHKT1;1 (GenBank accession number: MK986658), was isolated from melons based on genome data. The transcript of CmHKT1;1 was relatively more abundant in roots than in stems or leaves from melon seedlings. The tobacco transient expression system showed that CmHKT1;1 was plasma-membrane localized. Upon salt stress, CmHKT1;1 expression was more strongly upregulated in a salt-tolerant melon cultivar, ‘Bingxuecui’ (BXC) compared with a salt-sensitive cultivar, ‘Yulu’ (YL). Electrophysiological evidence demonstrated that CmHKT1;1 only transported Na+, rather than K+, when expressed in Xenopus laevis oocytes. Overexpression of CmHKT1;1 increased salt sensitivity in Saccharomyces cerevisiae and salt tolerance in Arabidopsis thaliana. Under NaCl treatments, transgenic Arabidopsis plants accumulated significantly lower concentrations of Na+ in shoots than wild type plants and showed a better K+/Na+ balance, leading to better Fv/Fm, root length, biomass, and enhanced plant growth. The CmHKT1;1 gene may serve as a useful candidate for improving crop salt tolerance.

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References

  • Adams E, Shin R (2014) Transport, signaling, and homeostasis of potassium and sodium in plants. J Integr Plant Biol 56:231–249. https://doi.org/10.1111/jipb.12159

    Article  CAS  PubMed  Google Scholar 

  • Ali A, Maggio A, Bressan RA, Yun DJ (2019) Role and functional differences of HKT1-type transporters in plants under salt stress. Int J Mol Sci 20(5):1059

    Article  CAS  Google Scholar 

  • Ali A, Khan IU, Jan M, Khan HA, Hussain S, Nisar M, Chung WS, Yun DJ (2018) The high-affinity potassium transporter EpHKT1;2 from the extremophile Eutrema parvula mediates salt tolerance. Front Plant Sci 9:1108

    Article  Google Scholar 

  • Ali Z, Park HC, Ali A, Oh DH, Aman R, Kropornicka A et al (2012) TsHKT1;2, a HKT1 homolog from the extremophile Arabidopsis relative Thellungiella salsuginea, shows K+ specificity in the presence of NaCl. Plant Physiol 158:1463–1474

    Article  CAS  Google Scholar 

  • Almeida DM, Oliveira MM, Saibo NJ (2017) Regulation of Na+ and K+ homeostasis in plants: towards improved salt stress tolerance in crop plants. Genet Mol Biol 40(1):326–345

    Article  CAS  Google Scholar 

  • Ben Amar S, Brini F, Sentenac H, Masmoudi K, Very AA (2014) Functional characterization in Xenopus oocytes of Na+ transport systems from durum wheat reveals diversity among two HKT1;4 transporters. J Exp Bot 65:213–222. https://doi.org/10.1093/jxb/ert361

    Article  CAS  PubMed  Google Scholar 

  • Berthomieu P, Conejero G, Nublat A, Brackenbury WJ, Lambert C, Savio C, Uozumi N, Oiki S, Yamada K, Cellier F, Gosti F, Simonneau T, Essah PA, Tester M, Very AA, Sentenac H, Casse F (2003) Functional analysis of AtHKT1 in Arabidopsis shows that Na+ recirculation by the phloem is crucial for salt tolerance. EMBO J 22:2004–2014. https://doi.org/10.1093/emboj/cdg207

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Botía P, Navarro JM, Cerdá A, Martínez V (2005) Yield and fruit quality of two melon cultivars irrigated with saline water at different stages of development. Eur J Agron 23:243–253. https://doi.org/10.1016/j.eja.2004.11.003

    Article  Google Scholar 

  • Byrt CS, Platten JD, Spielmeyer W, James RA, Lagudah ES, Dennis ES, Tester M, Munns R (2007) HKT1;5-like cation transporters linked to Na+ exclusion loci in wheat, Nax2 and Kna1. Plant Physiol 143:1918–1928. https://doi.org/10.1104/pp.106.093476

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Byrt CS, Xu B, Krishnan M, Lightfoot DJ, Athman A, Jacobs AK, Watson-Haigh NS, Plett D, Munns R, Tester M et al (2014) The Na+ transporter, TaHKT1;5-D, limits shoot Na+ accumulation in bread wheat. Plant J 80:516–526

    Article  CAS  Google Scholar 

  • Chen H, Chen X, Gu H, Wu B, Zhang H, Yuan X, Cui X (2014) GmHKT1;4, a novel soybean gene regulating Na+/K+ ratio in roots enhances salt tolerance in transgenic plants. Plant Growth Regul 73:299–308

    Article  CAS  Google Scholar 

  • Corratge-Faillie C, Jabnoune M, Zimmermann S, Very AA, Fizames C, Sentenac H (2010) Potassium and sodium transport in non-animal cells: the Trk/Ktr/HKT transporter family. Cell Mol Life Sci 67:2511–2532. https://doi.org/10.1007/s00018-010-0317-7

    Article  CAS  PubMed  Google Scholar 

  • Cotsaftis O, Plett D, Shirley N, Tester M, Hrmova M (2012) A two-staged model of Na+ exclusion in rice explained by 3D modeling of HKT transporters and alternative splicing. PLOS ONE 7:e39865

    Article  CAS  Google Scholar 

  • Davenport RJ, Munoz-Mayor A, Jha D, Essah PA, Rus A, Tester M (2007) The Na+ transporter AtHKT1;1 controls retrieval of Na+ from the xylem in Arabidopsis. Plant Cell Environ 30:497–507. https://doi.org/10.1111/j.1365-3040.2007.01637.x

    Article  CAS  PubMed  Google Scholar 

  • Deinlein U, Stephan AB, Horie T, Luo W, Xu G, Schroeder JI (2014) Plant salt-tolerance mechanisms. Trends Plant Sci 19:371–379. https://doi.org/10.1016/j.tplants.2014.02.001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gao JP, Chao DY, Lin HX (2007) Understanding abiotic stress tolerance mechanisms: recent studies on stress response in rice. J Integr Plant Biol 49:742–750. https://doi.org/10.1111/j.1744-7909.2007.00495.x

    Article  CAS  Google Scholar 

  • Garciadeblas B, Senn ME, Banuelos MA, Rodriguez-Navarro A (2003) Sodium transport and HKT transporters: the rice model. Plant J 34:788–801

    Article  CAS  Google Scholar 

  • Hamamoto S, Horie T, Hauser F, Deinlein U, Schroeder JI, Uozumi N (2015) HKT transporters mediate salt stress resistance in plants: from structure and function to the field. Curr Opin Biotechnol 32:113–120. https://doi.org/10.1016/j.copbio.2014.11.025

    Article  CAS  PubMed  Google Scholar 

  • Han Y, Yin S, Huang L, Wu X, Zeng J, Liu X, Qiu L, Munns R, Chen Z, Zhang G (2018) A sodium transporter HvHKT1; 1 confers salt tolerance in barley via regulating tissue and cell ion homeostasis. Plant Cell Physiol 59(10):1976–1989

    Article  CAS  Google Scholar 

  • Hassani D, Khalid M, Huang D, Zhang Y-D (2019) Morphophysiological and molecular evidence supporting the augmentative role of in mitigation of salinity in Cucumis melo L. Acta Biochimica et Biophysica Sinica 51(3):301–312

    Article  CAS  Google Scholar 

  • Hauser F, Horie TA (2010) conserved primary salt tolerance mechanism mediated by HKT transporters: a mechanism for sodium exclusion and maintenance of high K+/Na+ ratio in leaves during salinity stress. Plant Cell Environ. 33(4):552–565. https://doi.org/10.1111/j.1365-3040.2009.02056.x

    Article  CAS  PubMed  Google Scholar 

  • Horie T, Hauser F, Schroeder JI (2009) HKT transporter-mediated salinity resistance mechanisms in Arabidopsis and monocot crop plants. Trends Plant Sci 14:660–668. https://doi.org/10.1016/j.tplants.2009.08.009

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Huang LT, Zhao LN, Gao LW, Véry Anne-Aliénor, Sentenac Hervé, Zhang YD (2018) Constitutive expression of CmSKOR, an outward K+ channel gene from melon, in Arabidopsis thaliana involved in saline tolerance. Plant Sci 274:492–502

    Article  CAS  Google Scholar 

  • Huang S, Spielmeyer W, Lagudah ES, Munns R (2008) Comparative mapping of HKT genes in wheat, barley, and rice, key determinants of Na+ transport, and salt tolerance. J Exp Bot 59:927–937. https://doi.org/10.1093/jxb/ern033

    Article  CAS  PubMed  Google Scholar 

  • James RA, Davenport RJ, Munns R (2006) Physiological characterization of two genes for Na+ exclusion in durum wheat, Nax1 and Nax2. Plant Physiol 142:1537–1547. https://doi.org/10.1104/pp.106.086538

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Julkowska MM, Testerink C (2015) Tuning plant signaling and growth to survive salt. Trends Plant Sci 20:586–594. https://doi.org/10.1016/j.tplants.2015.06.008

    Article  CAS  PubMed  Google Scholar 

  • Kobayashi NI, Yamaji N, Yamamoto H, Okubo K, Ueno H, Costa A, Tanoi K, Matsumura H, Fujii-Kashino M, Horiuchi T, Nayef MA, Shabala S, An G, Ma JF, Horie T (2017) OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice. Plant J 91:657–670. https://doi.org/10.1111/tpj.13595

    Article  CAS  PubMed  Google Scholar 

  • Kronzucker HJ, Britto DT (2011) Sodium transport in plants: a critical review. New Phytol 189:54–81

    Article  CAS  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25:402–408

    Article  CAS  Google Scholar 

  • Liu W, Fairbairn DJ, Schachtman RDP (2001) Characterization of two hkt1 homologues from eucalyptus camaldulensis that display intrinsic osmosensing capability. Plant Physiol 127(1):283–294

    Article  CAS  Google Scholar 

  • Mäser P, Eckelman B, Vaidyanathan R, Horie T, Fairbairn DJ, Kubo M, Yamagami M, Yamaguchi K, Nishimura M, Uozumi N (2002a) Altered shoot/root Na+ distribution and bifurcating salt sensitivity in Arabidopsis by genetic disruption of the Na+ transporter AtHKT1. FEBS Lett 531:157–161

    Article  Google Scholar 

  • Mäser P, Hosoo Y, Goshima S, Horie T, Eckelman B, Yamada K, Yoshida K, Bakker EP, Shinmyo A, Oiki S (2002b) Glycine residues in potassium channel-like selectivity filters determine potassium selectivity in four-loop-per-subunit HKT transporters from plants. Proc Natl Acad Sci USA 99:6428–6433

    Article  Google Scholar 

  • Mendlinger S, Pasternak D (1992) Effect of time of salinization on flowering, yield and fruit quality factors in melon, Cucumis melo L. J Hortic Sci 67:529–534

    Article  Google Scholar 

  • Moller IS, Gilliham M, Jha D, Mayo GM, Roy SJ, Coates JC, Haseloff J, Tester M (2009) Shoot Na+ exclusion and increased salinity tolerance engineered by cell type-specific alteration of Na+ transport in Arabidopsis. Plant Cell 7:2163–2178

    Article  Google Scholar 

  • Munns R, James RA, Xu B, Athman A, Conn SJ, Jordans C, Byrt CS, Hare RA, Tyerman SD, Tester M, Plett D, Gilliham M (2012) Wheat grain yield on saline soils is improved by an ancestral Na+ transporter gene. Nat Biotechnol 30:360–364. https://doi.org/10.1038/nbt.2120

    Article  CAS  PubMed  Google Scholar 

  • Nishijima T, Furuhashi M, Sakaoka S, Morikami A, Tsukagoshi H (2017) Ectopic expression of Mesembryanthemum crystallinum sodium transporter McHKT2 provides salt stress tolerance in Arabidopsis thaliana. Biosci Biotechnol Biochem 81:2139–2144

    Article  CAS  Google Scholar 

  • Platten JD, Cotsaftis O, Berthomieu P, Bohnert H, Davenport RJ, Fairbairn DJ, Horie T, Leigh RA, Lin HX, Luan S, Maser P, Pantoja O, Rodriguez-Navarro A, Schachtman DP, Schroeder JI, Sentenac H, Uozumi N, Very AA, Zhu JK, Dennis ES, Tester M (2006) Nomenclature for HKT transporters, key determinants of plant salinity tolerance. Trends Plant Sci 11:372–374. https://doi.org/10.1016/j.tplants.2006.06.001

    Article  CAS  PubMed  Google Scholar 

  • Ren ZH, Gao JP, Li LG, Cai XL, Huang W, Chao DY, Zhu MZ, Wang ZY, Luan S, Lin HX (2005) A rice quantitative trait locus for salt tolerance encodes a sodium transporter. Nat Genet 37:1141–1146. https://doi.org/10.1038/ng1643

    Article  CAS  PubMed  Google Scholar 

  • Ren ZJ, Liu Y, Kang D, Fan KJ, Wang CY, Wang GY, Liu YJ (2015) Two alternative splicing variants of maize HKT1;1 confer salt tolerance in transgenic tobacco plants. Plant Cell Tissue Organ Cult 123:569–578

    Article  CAS  Google Scholar 

  • Ruan CJ, da Silva JAT, Mopper S, Pei Q, Lutts S (2010) Halophyte improvement for a salinized world. Crit Rev Plant Sci 29:329–359. https://doi.org/10.1080/07352689.2010.524517

    Article  CAS  Google Scholar 

  • Rus A, Lee BH, Munoz-Mayor A, Sharkhuu A, Miura K, Zhu JK, Bressan RA, Hasegawa PM (2004) AtHKT1 facilitates Na+ homeostasis and K+ nutrition in planta. Plant Physiol 136:2500–2511. https://doi.org/10.1104/pp.104.042234

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Sarabi B, Bolandnazar S, Ghaderi N, Ghashghaie J (2017) Genotypic differences in physiological and biochemical responses to salinity stress in melon (Cucumis melo. L) plants: prospects for selection of salt tolerant landraces. Plant Physiol Biochem 119:294–311. https://doi.org/10.1016/j.plaphy.2017.09.006

    Article  CAS  PubMed  Google Scholar 

  • Schachtman DP, Schroeder JI (1994) Structure and transport mechanism of a high-affinity potassium uptake transporter from higher plants. Nature 370:655–658. https://doi.org/10.1038/370655a0

    Article  CAS  PubMed  Google Scholar 

  • Shabala S (2013) Learning from halophytes: physiological basis and strategies to improve abiotic stress tolerance in crops. Ann Bot 112:1209–1221. https://doi.org/10.1093/aob/mct205

    Article  PubMed  PubMed Central  Google Scholar 

  • Su Y, Luo W, Lin W, Ma L, Kabir M (2015) Model of cation transportation mediated by high-affinity potassium transporters (hkts) in higher plants. Biological Procedures Online 17(1):1–13

    Article  Google Scholar 

  • Sun J, Cao H, Cheng J, He X, Sohail H, Niu M, Huang Y, Bie Z (2018) Pumpkin CmHKT1;1 controls shoot Na+ accumulation via limiting Na+ transport from rootstock to scion in grafted cucumber. Int J Mol Sci 19(9):2648. https://doi.org/10.3390/ijms19092648

    Article  CAS  PubMed Central  Google Scholar 

  • Sunarpi Horie T, Motoda J, Kubo M, Yang H, Yoda K, Horie R, Chan WY, Leung HY, Hattori K, Konomi M, Osumi M, Yamagami M, Schroeder JI, Uozumi N (2005) Enhanced salt tolerance mediated by AtHKT1 transporter-induced Na unloading from xylem vessels to xylem parenchyma cells. Plant J 44:928–938. https://doi.org/10.1111/j.1365-313X.2005.02595.x

    Article  CAS  PubMed  Google Scholar 

  • Tedeschi A, Lavini A, Riccardi M, Pulvento C, d’Andria R (2011) Melon crops (Cucumis melo L., cv. Tendral) grown in a mediterranean environment under saline-sodic conditions: Part I. Yield and quality. Agric Water Manage 98(9):1329–1338

    Article  Google Scholar 

  • Tester M, Davenport R (2003) Na+ tolerance and Na+ transport in higher plants. Ann Bot 91:503–527

    Article  CAS  Google Scholar 

  • Thomson MJ, de Ocampo M, Egdane J, Rahman MA, Sajise AG, Adorada DL, Tumimbang-Raiz E, Blumwald E, Seraj ZI, Singh RK, Gregorio GB, Ismail AM (2010) Characterizing the saltol quantitative trait locus for salinity tolerance in rice. Rice 3:148–160. https://doi.org/10.1007/s12284-010-9053-8

    Article  Google Scholar 

  • Tilman D, Balzer C, Hill J, Befort BL (2011) Global food demand and the sustainable intensification of agriculture. Proc Natl Acad Sci USA 108:20260–20264. https://doi.org/10.1073/pnas.1116437108

    Article  PubMed  Google Scholar 

  • Uozumi N, Kim EJ, Rubio F, Yamaguchi T, Muto S, Tsuboi A, Bakker EP, Nakamura T, Schroeder JI (2000) The Arabidopsis HKT1 gene homolog mediates inward Na+ currents in Xenopus laevis oocytes and Na+ uptake in Saccharomyces cerevisiae. Plant Physiol 122(4):1249–1260

    Article  CAS  Google Scholar 

  • Véry AA, Gaymard F, Bosseux C, Sentenac H, Thibaud JB (1995) Expression of a cloned plant K+ channel in Xenopus oocytes: analysis of macroscopic currents. Plant J 7:321–332

    Article  Google Scholar 

  • Wang LM, Zhang LD, Chen JB, Huang DF, Zhang YD (2016) Physiological analysis and transcriptome comparison of two muskmelon (Cucumis melo L.) cultivars in response to salt stress. Genet Mol Res 15(3):1–18. https://doi.org/10.4238/gmr.15038738

    Article  CAS  Google Scholar 

  • Wang L, Wei S, Chen J, Zhang Y, Huang D (2013) Regulation of the inward rectifying K+ channel MIRK and ion distribution in two melon cultivars (Cucumis melo L.) under NaCl salinity stress. Acta Physiol Plant 35:2789–2800. https://doi.org/10.1007/s11738-013-1311-0

    Article  CAS  Google Scholar 

  • Wang R, Jing W, Xiao L (2015) The rice high-affinity potassium transporter1;1 is involved in salt tolerance and regulated by an MYB-type transcription factor. Plant Physiol 168(3):1076–1090. https://doi.org/10.1104/pp.15.00298

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang T, Gassmann W, Rubio F, Schroeder JI, Glass ADM (1998) Rapid up-regulation of HKT1, a high-affinity potassium transporter gene, in roots of barley and wheat following withdrawal of potassium. Plant Physiol 118:651–659

    Article  Google Scholar 

  • Wang TT, Ren ZJ, Liu ZQ, Feng X, Guo RQ, Li BG, Li LG, Jing HC (2014) SbHKT1;4, a member of the high-affinity potassium transporter gene family from Sorghum bicolor, functions to maintain optimal Na+ /K+ balance under Na+ stress. J Integr Plant Biol 56:315–332. https://doi.org/10.1111/jipb.12144

    Article  CAS  PubMed  Google Scholar 

  • Yasar F, Kusvuran S, Ellialtioglu S (2006) Determination of anti-oxidant activities in some melon (Cucumis melo L.) varieties and cultivars under salt stress. J Hortic Sci Biotech 81:627–630. https://doi.org/10.1080/14620316.2006.11512115

    Article  CAS  Google Scholar 

  • Zhang YD, Very AA, Wang LM, Deng YW, Sentenac H, Huang DF (2011) A K+ channel from salt-tolerant melon inhibited by Na+. New Phytol 189:856–868. https://doi.org/10.1111/j.1469-8137.2010.03526.x

    Article  CAS  PubMed  Google Scholar 

  • Zhao LN, Zhang FR, Liu B, Yang SL, Xiong X, Hassani D, Zhang YD (2019) CmRAV1 shows differential expression in two melon (Cucumis melo L.) cultivars and enhanced salt tolerance in transgenic Arabidopsis plants. Acta Biochim Biophys Sin 51(11):1123–1133. https://doi.org/10.1093/abbs/gmz107

    Article  PubMed  Google Scholar 

  • Zhu M, Shabala L, Cuin TA, Huang X, Zhou M, Munns R, Shabala S (2016) Nax loci affect SOS1-like Na+/H+ exchanger expression and activity in wheat. J Exp Bot 67:835–844. https://doi.org/10.1093/jxb/erv493

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We thanked Dr. Limin Wang help doing the voltage-clamp experiments in B&PMP (Biochimie et Physiologie Moléculaires des Plantes) in France. This work was sponsored by the National Nature Science Foundation of China (NSFC, 31372079).

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  1. Li-Wei Gao, Sen-Lin Yang have contributed equally to this work.

Authors and Affiliations

  1. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Li-Wei Gao, Sen-Lin Yang, Dan-Feng Huang & Yi-Dong Zhang

  2. Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, China

    Dan-Feng Huang & Yi-Dong Zhang

  3. Shanghai Agrobiological Gene Center, Shanghai, 201106, People’s Republic of China

    Shi-Wei Wei

  4. Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People’s Republic of China

    Yi-Dong Zhang

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Contributions

L-W G conducted the experiments and wrote the draft manuscript. S-L Y participated in the experiment of subcellular localization and yeast test. S-W W participated in the preparation of the plant material and wrote the part of the discussion. Y-D Z designed the research plan together with D-F H, revised the paper, analyzed the electrophysiological experiments and part of the data. All of authors in this study read and approved the manuscript.

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Correspondence to Yi-Dong Zhang.

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11103_2020_1011_MOESM3_ESM.tif

Independent candidates from the 35 overexpression lines were choosed for detection. OE1 and OE6 were selected for the following experiments. Supplementary material 3 (TIFF 134 kb)

The CmHKT1;1 gene expression in the OE lines under NaCl treatments. Supplementary material 4 (TIFF 799 kb)

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Gao, LW., Yang, SL., Wei, SW. et al. Supportive role of the Na+ transporter CmHKT1;1 from Cucumis melo in transgenic Arabidopsis salt tolerance through improved K+/Na+ balance. Plant Mol Biol 103, 561–580 (2020). https://doi.org/10.1007/s11103-020-01011-0

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