Abstract
Key message
Two homologous, chloroplast located CAAX proteases were identified to be functional redundancy in determining soybean leaf color, and they probably play essential roles in regulating light harvesting and absorption during photosynthesis process.
Abstract
Leaf color mutants are ideal materials for studying photosynthesis and chlorophyll metabolism. The soybean [Glycine max (L.) Merr.] yellowing leaf (yl) variation is a recombinant mutant characterized by yellow foliage, which derived from the specific cross between green seed-coated and yellow seed-coated soybean varieties. Molecular cloning and subsequent gene silencing revealed that the yellow leaf trait of yl was controlled by two recessive nuclear genes, glyma11g04660 and glyma01g40650, named as YL1 and YL2 respectively, and the latter was confirmed to be same as the earlier reported green seed-coat gene G. Both YL1 and YL2 belonged to chloroplast-located proteases possessing Abi domain, and these genes were expressed in various tissues, especially in young leaves. In yl, the expression of YL1 and YL2 were suppressed in most tissues, and the young leaf of yl presented an increased maximal photochemical efficiency (Fv/Fm) as well as enhanced net photosynthesis activity (Pn), indicating that YL1 and YL2 are involved in light absorption regulation during photosynthesis process. Collectively, the identification and description of YL1 and YL2 in our study provides insights for the regulatory mechanism of photosynthesis process, and these findings will further assist to clarify the close relationship between photosynthesis and chlorophyll metabolism.
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Acknowledgements
We are grateful to Prof. Kang Liu (College of Agriculture, Nanjing Agricultural University, NJAU) for providing the TRV-VIGS system, Prof. Xiaoming Xu (College of Plant Biology, NJAU) for providing advice on photosynthesis experiment design, and Prof. Wayne Parrott and Dr. Peter Lafayette (Center for applied and genetics technology, UGA) for their valuable suggestions and language improvement.
Funding
This work was supported by the National Key R & D Program of China (2016YFD0100201), the National Transgenic Major Program of China (2016ZX08004001), the National Natural Science Foundation of China (Grant Nos. 31271750, 31571691, 31871646), the MOE Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT_17R55), the Fundamental Research Funds for the Central Universities (KYT201801), the Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP) Program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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ML and TZ organized the entire of this research. ML, TZ and JG designed the research. YW, ZN, and JK performed the experiments and analyzed data. ML wrote the manuscript. TZ and JB revised the paper. All authors read and approved the manuscript.
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Liu, M., Wang, Y., Nie, Z. et al. Double mutation of two homologous genes YL1 and YL2 results in a leaf yellowing phenotype in soybean [Glycine max (L.) Merr]. Plant Mol Biol 103, 527–543 (2020). https://doi.org/10.1007/s11103-020-01008-9
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DOI: https://doi.org/10.1007/s11103-020-01008-9