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PSBR1, encoding a mitochondrial protein, is regulated by brassinosteroid in moso bamboo (Phyllostachys edulis)

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Abstract

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PSBR1 is a moso bamboo gene negatively regulated by brassinosteroid, which encodes a mitochondrial localized protein. Overexpression of PSBR1 leads to growth inhibition in various growth progresses in Arabidopsis.

Abstract

The young shoot of moso bamboo (Phyllostachys edulis) is known as one of the fastest growing plant organs. The roles of phytohormones in the fast-growth of bamboo shoot are not fully understood. Brassinosteroids (BRs) are a group of growth-promoting steroid hormones that play important roles in cell elongation and division. While BR related genes are highly enriched in fast-growing internodes in moso bamboo, the functions of BR in the fast-growth process is not understood at the molecular level. Here, we identified a poaceae specific gene, PSBR1 (Poaceae specific and BR responsive gene 1) from the moso bamboo genome. PSBR1 was highly expressed in the stem and leaves of bamboo seedling, and the elongating nodes of fast-growing bamboo shoot. PSBR1′s expression is increased by BR biosynthesis inhibitor propiconazole but decreased by BR treatment. PSBR1 encodes a novel protein that is localized to the mitochondria in tobacco and bamboo protoplast. The Arabidopsis transgenic plants overexpressing PSBR1 show growth inhibition in both vegetative and reproductive stages. This study suggests that PSBR1 is a BR regulated mitochondrial protein in bamboo, which inhibits plant growth when overexpressed in Arabidopsis.

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Acknowledgements

We thank Dr. Jia-Ying Zhu for editing the manuscript. This work was supported by a grant from the Natural Science Foundation of Fujian province (NO. 2016J01089) and FAFU-International Collaborative Program (KXb16005A) to W.W., the National Natural Science Foundation of China grant (NO. 31500226) to X.Y., the China Postdoctoral Science Foundation (2018M642551) to ZZ.Z., and Program for scientific and technological innovation team for universities of Fujian province (No. 118/KLA18069A) to Q.Z.. The funding body had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.

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  1. Zejun Guo and Zhe Zhang have contributed equally to this work.

Authors and Affiliations

  1. Basic Forestry and Proteomics Research Center, College of Forestry, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Fuzhou, 350002, Fujian, China

    Zejun Guo, Zhe Zhang, Xuelian Yang, Kuixing Yin, Yitao Chen, Zhenzhen Zhang, Kihye Shin, Qiang Zhu & Wenfei Wang

  2. Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, 94305, USA

    Xuelian Yang & Zhi-Yong Wang

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  1. Zejun Guo
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Contributions

WW and ZYW designed the research program. ZG, ZZ, XY, KY and YC prepared materials. ZG performed research. ZG, ZZ, ZYW, KS, ZZZ, QZ and WW analyzed data. ZG, ZZ and WW wrote the drafting of manuscript. All authors have read, revised and approved the final manuscript.

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Correspondence to Wenfei Wang.

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11103_2020_975_MOESM1_ESM.tif

Supplemental Figure 1: Whole cell image of PSBR1 subcellular localization in tobacco, Arabidopsis and bamboo protoplast. a-l Subcellular localization of PSBR1 in Nicotiana benthamiana cells. a PSBR1-YFP signals. b DAPI stain signals. c Peroxisome maker mCherry-PTS1 signals. d Chlorophyll auto-fluorescence signals. e BF, bright-field images. f Merged of (a) and (b). g Merged of (a) and (c). h Merged of (a) and (d). i PSBR1-YFP signals. j Mitochondrial maker CoxIV-mCherry signals. k Merged of (i) and (j). l BF. Bar = 20 μm. m-p Subcellular localization of PSBR1 in Arabidopsis. m PSBR1-YFP signals. n Mito tracker orange signals. o Merged of (m) and (n). p BF. Bar = 20 μm. q-t Subcellular localization of PSBR1 in a protoplast of Moso bamboo. q PSBR1-YFP signals. r Mito tracker orange signals. s Merged of (q) and (r). t BF. Bar = 5 μm (TIF 10530 kb)

11103_2020_975_MOESM2_ESM.tif

Supplemental Figure 2: Whole cell image of subcellular localization of dissected PSBR1 fragments. a Diagram of N- and C- terminal fragments of PSBR1 for YFP fusion. b, f, j and n YFP signals. c, g, k and o Mitochondrial maker CoxIV-mCherry signals. d, h, l and p Merged images of mCherry and YFP signals. e, i, m and q BF. Bar = 20 μm (TIF 13792 kb)

Supplemental Table 1: Primers used in this article (XLSX 10 kb)

Supplemental Table 2: Amino acid sequences of PSBR1 orthologous (TXT 0 kb)

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Guo, Z., Zhang, Z., Yang, X. et al. PSBR1, encoding a mitochondrial protein, is regulated by brassinosteroid in moso bamboo (Phyllostachys edulis). Plant Mol Biol 103, 63–74 (2020). https://doi.org/10.1007/s11103-020-00975-3

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