Transcriptome analysis of sugar beet (Beta vulgaris L.) in response to alkaline stress

Zou, Chunlei; Liu, Dan; Wu, Peiran; Wang, Yubo; Gai, Zhijia; Liu, Lei; Yang, Fangfang; Li, Caifeng; Guo, Guanghao

doi:10.1007/s11103-020-00971-7

Published: 10 February 2020

Transcriptome analysis of sugar beet (Beta vulgaris L.) in response to alkaline stress

Chunlei Zou¹,
Dan Liu¹,
Peiran Wu¹,
Yubo Wang¹,
Zhijia Gai²,
Lei Liu¹,
Fangfang Yang¹,
Caifeng Li¹ &
Guanghao Guo¹

Plant Molecular Biology volume 102, pages 645–657 (2020)Cite this article

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Abstract

Key message

RNA-seq was used to analyze the transcriptional changes in sugar beet (Beta vulgaris L.) triggered by alkaline solution to elucidate the molecular mechanism underlying alkaline tolerance in sugar beet. Several differentially expressed genes related to stress tolerance were identified. Our results provide a valuable resource for the breeding of new germplasms with high alkaline tolerance.

Abstract

Alkalinity is a highly stressful environmental factor that limits plant growth and production. Sugar beet own the ability to acclimate to various abiotic stresses, especially salt and alkaline stress. Although substantial previous studies on response of sugar beet to saline stress has been conducted, the expressions of alkali-responsive genes in sugar beet have not been comprehensively investigated. In this study, we conducted transcriptome analysis of leaves in sugar beet seedlings treated with alkaline solutions for 0 day (control, C), 3 days (short-term alkaline treatment, ST) and 7 days (long-term alkaline treatment, LT). The clean reads were obtained and assembled into 25,507 unigenes. Among them, 975 and 383 differentially expressed genes (DEGs) were identified in the comparison groups ST_vs_C and LT_vs_C, respectively. Gene ontology (GO) analysis revealed that oxidation–reduction process and lipid metabolic process were the most enriched GO term among the DEGs in ST_vs_C and LT_vs_C, respectively. According to Kyoto Encyclopedia of Genes and Genomes pathway, carbon fixation in photosynthetic organisms pathway were significantly enriched under alkaline stress. Besides, expression level of genes encoding d-3-phosphoglycerate dehydrogenase 1, glutamyl-tRNA reductase 1, fatty acid hydroperoxide lyase, ethylene-insensitive protein 2, metal tolerance protein 11 and magnesium-chelatase subunit ChlI, etc., were significantly altered under alkaline stress. Additionally, among the DEGs, 136 were non-annotated genes and 24 occurred with differential alternative splicing. Our results provide a valuable resource on alkali-responsive genes and should benefit the improvement of alkaline stress tolerance in sugar beet.

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Acknowledgement

This research was supported by National Natural Science Foundation of China (31671622) and Technical System of Sugar Industry of China (CARS-170201).

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College of Agronomy, Northeast Agricultural University, Harbin, China
Chunlei Zou, Dan Liu, Peiran Wu, Yubo Wang, Lei Liu, Fangfang Yang, Caifeng Li & Guanghao Guo
Jiamusi Branch, Heilongjiang Academy of Agricultural Sciences, Jiamusi, China
Zhijia Gai

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Chunlei Zou
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Dan Liu
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Peiran Wu
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Yubo Wang
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Zhijia Gai
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Lei Liu
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Fangfang Yang
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Caifeng Li
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Guanghao Guo
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Contributions

CLZ, DL, PRW and CFL conceived the study. CLZ, YBW and CFL designed the sequence data sets. CLZ, and DL analyzed the data. CLZ, ZJG, LL, FFY and GHG drafted the manuscript. All the authors contributed to editing and revising the manuscript.

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Correspondence to Caifeng Li.

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Zou, C., Liu, D., Wu, P. et al. Transcriptome analysis of sugar beet (Beta vulgaris L.) in response to alkaline stress. Plant Mol Biol 102, 645–657 (2020). https://doi.org/10.1007/s11103-020-00971-7

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Received: 07 July 2018
Accepted: 18 January 2020
Published: 10 February 2020
Issue Date: April 2020
DOI: https://doi.org/10.1007/s11103-020-00971-7

Keywords

Transcriptome
Beta vulgaris L.
Alkaline stress
Oxidation–reduction
Non-annotated genes
Alternative splicing

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Electronic supplementary material

Supplementary file1 (DOCX 17 kb)

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