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Plant Molecular Biology

, Volume 96, Issue 6, pp 607–625 | Cite as

TaEDS1 genes positively regulate resistance to powdery mildew in wheat

  • Guiping Chen
  • Bo Wei
  • Guoliang Li
  • Caiyan Gong
  • Renchun Fan
  • Xiangqi Zhang
Article

Abstract

Key message

Three EDS1 genes were cloned from common wheat and were demonstrated to positively regulate resistance to powdery mildew in wheat.

Abstract

The EDS1 proteins play important roles in plant basal resistance and TIR-NB-LRR protein-triggered resistance in dicots. Until now, there have been very few studies on EDS1 in monocots, and none in wheat. Here, we report on three common wheat orthologous genes of EDS1 family (TaEDS1-5A, 5B and 5D) and their function in powdery mildew resistance. Comparisons of these genes with their orthologs in diploid ancestors revealed that EDS1 is a conserved gene family in Triticeae. The cDNA sequence similarity among the three TaEDS1 genes was greater than 96.5%, and they shared sequence similarities of more than 99.6% with the respective orthologs from diploid ancestors. The phylogenetic analysis revealed that the EDS1 family originated prior to the differentiation of monocots and dicots, and EDS1 members have since undergone clear structural differentiation. The transcriptional levels of TaEDS1 genes in the leaves were obviously higher than those of the other organs, and they were induced by Blumeria graminis f. sp. tritici (Bgt) infection and salicylic acid (SA) treatment. The BSMV-VIGS experiments indicated that knock-down the transcriptional levels of the TaEDS1 genes in a powdery mildew-resistant variety of common wheat compromised resistance. Contrarily, transient overexpression of TaEDS1 genes in a susceptible common wheat variety significantly reduced the haustorium index and attenuated the growth of Bgt. Furthermore, the expression of TaEDS1 genes in the Arabidopsis mutant eds1-1 complemented its susceptible phenotype to powdery mildew. The above evidences strongly suggest that TaEDS1 acts as a positive regulator and confers resistance against powdery mildew in common wheat.

Keywords

Wheat TaEDS1 gene Powdery mildew resistance BSMV-VIGS Transient expression Functional complementation 

Notes

Acknowledgements

We thank Professor Xiayu Duan and Professr Yilin Zhou (Institute of Plant Protection, CAAS) for providing seeds of common wheat varieties Shangeda and Chancellor, and Blumeria graminis f. sp. tritici isolate E18; Professor Dingzhong Tang (State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, CAS) for providing seeds of common wheat varieties Xiaobaidongmai and Arabidopsis mutant pad4-1; Dr. Yiwen Li (Center for Molecular Agrobiology, Institute of Genetics and Developmental Biology, CAS) for providing seeds of Chinese Spring Nulli-tetrasomic lines and diploid ancestor species of common wheat, and Professor Zhiying Ma (Agriculture University of Hebei, China) for providing the seeds of Arabidopsis thaliana ecotype Ws and mutant eds1-1. We are grateful to Professor Andy Jackson (University of California-Berkley, USA) for providing full-length infectious cDNA clones of BSMV. We would also like to thank Professor Hongjie Li (Institute of Crop Science, CAAS) for help with the inoculation of powdery mildew fungus; Professor Qianhua Shen (State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, CAS) for guidance in transient expression experiment of TaEDS1 genes in wheat cell; and Professor Daowen Wang (State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, CAS) for help with the manuscript revision. This work was supported by Grants from the Ministry of Science and Technology of the People’s Republic of China (2014BA525B03), the National Natural Science Foundation of China (31471484) and the State Key Laboratory of Plant Cell and Chromosome Engineering, (PCCE-TD-2012-01).

Author Contributions

XZ and RF conceived the project and designed experiments; GC, RF, BW, GL and CG performed experiments; GC, RF and XZ analysed experiment data and wrote the manuscript.

Supplementary material

11103_2018_718_MOESM1_ESM.pdf (823 kb)
Supplementary material 1 (PDF 823 KB)

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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina
  2. 2.Department of Life ScienceTangshan Normal UniversityTangshanChina
  3. 3.Institute of Genetics and PhysiologyHebei Academy of Agriculture and Forestry SciencesShijiazhuangChina
  4. 4.University of Chinese Academy of SciencesBeijingChina

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