TaEDS1 genes positively regulate resistance to powdery mildew in wheat
- 895 Downloads
Three EDS1 genes were cloned from common wheat and were demonstrated to positively regulate resistance to powdery mildew in wheat.
The EDS1 proteins play important roles in plant basal resistance and TIR-NB-LRR protein-triggered resistance in dicots. Until now, there have been very few studies on EDS1 in monocots, and none in wheat. Here, we report on three common wheat orthologous genes of EDS1 family (TaEDS1-5A, 5B and 5D) and their function in powdery mildew resistance. Comparisons of these genes with their orthologs in diploid ancestors revealed that EDS1 is a conserved gene family in Triticeae. The cDNA sequence similarity among the three TaEDS1 genes was greater than 96.5%, and they shared sequence similarities of more than 99.6% with the respective orthologs from diploid ancestors. The phylogenetic analysis revealed that the EDS1 family originated prior to the differentiation of monocots and dicots, and EDS1 members have since undergone clear structural differentiation. The transcriptional levels of TaEDS1 genes in the leaves were obviously higher than those of the other organs, and they were induced by Blumeria graminis f. sp. tritici (Bgt) infection and salicylic acid (SA) treatment. The BSMV-VIGS experiments indicated that knock-down the transcriptional levels of the TaEDS1 genes in a powdery mildew-resistant variety of common wheat compromised resistance. Contrarily, transient overexpression of TaEDS1 genes in a susceptible common wheat variety significantly reduced the haustorium index and attenuated the growth of Bgt. Furthermore, the expression of TaEDS1 genes in the Arabidopsis mutant eds1-1 complemented its susceptible phenotype to powdery mildew. The above evidences strongly suggest that TaEDS1 acts as a positive regulator and confers resistance against powdery mildew in common wheat.
KeywordsWheat TaEDS1 gene Powdery mildew resistance BSMV-VIGS Transient expression Functional complementation
We thank Professor Xiayu Duan and Professr Yilin Zhou (Institute of Plant Protection, CAAS) for providing seeds of common wheat varieties Shangeda and Chancellor, and Blumeria graminis f. sp. tritici isolate E18; Professor Dingzhong Tang (State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, CAS) for providing seeds of common wheat varieties Xiaobaidongmai and Arabidopsis mutant pad4-1; Dr. Yiwen Li (Center for Molecular Agrobiology, Institute of Genetics and Developmental Biology, CAS) for providing seeds of Chinese Spring Nulli-tetrasomic lines and diploid ancestor species of common wheat, and Professor Zhiying Ma (Agriculture University of Hebei, China) for providing the seeds of Arabidopsis thaliana ecotype Ws and mutant eds1-1. We are grateful to Professor Andy Jackson (University of California-Berkley, USA) for providing full-length infectious cDNA clones of BSMV. We would also like to thank Professor Hongjie Li (Institute of Crop Science, CAAS) for help with the inoculation of powdery mildew fungus; Professor Qianhua Shen (State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, CAS) for guidance in transient expression experiment of TaEDS1 genes in wheat cell; and Professor Daowen Wang (State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, CAS) for help with the manuscript revision. This work was supported by Grants from the Ministry of Science and Technology of the People’s Republic of China (2014BA525B03), the National Natural Science Foundation of China (31471484) and the State Key Laboratory of Plant Cell and Chromosome Engineering, (PCCE-TD-2012-01).
XZ and RF conceived the project and designed experiments; GC, RF, BW, GL and CG performed experiments; GC, RF and XZ analysed experiment data and wrote the manuscript.
- Hein I, Barciszewska-Pacak M, Hrubikova K, Williamson S, Dinesen M, Soenderby IE, Sundar S, Jarmolowski A, Shirasu K, Lacomme C (2005) Virus-induced gene silencing-based functional characterization of genes associated with powdery mildew resistance in barley. Plant Physiol 138:2155–2164CrossRefPubMedPubMedCentralGoogle Scholar
- Morgounov A, Tufan HA, Sharma R, Akin B, Bagci A, Braun H-J, Kaya Y, Keser M, Payne TS, Sonder K, McIntosh R (2012) Global incidence of wheat rusts and powdery mildew during 1969–2010 and durability of resistance of winter wheat variety Bezostaya1. Eur J Plant Pathol 132:323–340CrossRefGoogle Scholar
- Ohyanagi H, Tanaka T, Sakai H, Shigemoto Y, Yamaguchi K, Habara T, Fujii Y, Antonio BA, Nagamura Y, Imanishi T, Ikeo K, Itoh T, Gojobori T, Sasaki T (2006) The rice annotation project database (RAP-DB): hub for Oryza sativa ssp. japonica genome information. Nucleic Acids Res 34:D741–D744CrossRefPubMedGoogle Scholar
- Rietz S, Stamm A, Malonek S, Wagner S, Becker D, Medina-Escobar N, Vlot AC, Feys BJ, Niefind K, Parker JE (2011) Different roles of Enhanced Disease Susceptibility1 (EDS1) bound to and dissociated from Phytoalexin Deficient 4 (PAD4) in Arabidopsis immunity. New Phytol 191:107–119CrossRefPubMedGoogle Scholar
- Schornack S, Ballvora A, Gurlebeck D, Peart J, Baulcombe D, Ganal M, Baker B, Bonas U, Lahaye T (2004) The tomato resistance protein Bs4 is a predicted non-nuclear TIR-NB-LRR protein that mediates defense responses to severely truncated derivatives of AvrBs4 and overexpressed AvrBs3. Plant J 37:46–60CrossRefPubMedGoogle Scholar
- Wang GF, Wei XN, Fan RC, Zhou HB, Wang XP, Yu CM, Dong LL, Dong ZY, Wang XJ, Kang ZS, Shen QH, Wang DW, Zhang XQ (2011) Molecular analysis of common wheat genes encoding three types of cytosolic heat shock protein 90 (Hsp90): functional involvement of cytosolic Hsp90s in the control of wheat seedling growth and disease resistance. New Phytol 191:418–431CrossRefPubMedGoogle Scholar
- Xu XD (2017) Identification and mapping of powdery mildew resistance genes from five wheat landraces. Doctoral dissertation, China Agricultural UniversityGoogle Scholar
- Xue F, Zhai WW, Duan XY, Zhou YL, Ji WQ (2009) Microsatellite mapping of powdery mildew resistance gene in wheat landrace Xiaobaidong. Acta Agron Sin 35:1806–1811Google Scholar
- Zhou HB, Li SF, Deng ZY, Wang XP, Chen T, Zhang JS, Chen SY, Ling HQ, Zhang AM, Wang DW, Zhang XQ (2007) Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection. Plant J 52:420–434CrossRefPubMedGoogle Scholar