Plant Molecular Biology

, Volume 96, Issue 4–5, pp 457–471 | Cite as

A banana NAC transcription factor (MusaSNAC1) impart drought tolerance by modulating stomatal closure and H2O2 content

Article

Abstract

Key message

MusaSNAC1 function in H2O2 mediated stomatal closure and promote drought tolerance by directly binding to CGT[A/G] motif in regulatory region of multiple stress-related genes.

Abstract

Drought is a abiotic stress-condition, causing reduced plant growth and diminished crop yield. Guard cells of the stomata control photosynthesis and transpiration by regulating CO2 exchange and water loss, thus affecting growth and crop yield. Roles of NAC (NAM, ATAF1/2 and CUC2) protein in regulation of stress-conditions has been well documented however, their control over stomatal aperture is largely unknown. In this study we report a banana NAC protein, MusaSNAC1 which induced stomatal closure by elevating H2O2 content in guard cells during drought stress. Overexpression of MusaSNAC1 in banana resulted in higher number of stomata closure causing reduced water loss and thus elevated drought-tolerance. During drought, expression of GUS (β-glucuronidase) under P MusaSNAC1 was remarkably elevated in guard cells of stomata which correlated with its function as a transcription factor regulating stomatal aperture closing. MusaSNAC1 is a transcriptional activator belonging to SNAC subgroup and its 5′-upstream region contain multiple Dof1 elements as well as stress-associated cis-elements. Moreover, MusaSNAC1 also regulate multiple stress-related genes by binding to core site of NAC-proteins CGT[A/G] in their 5′-upstream region. Results indicated an interesting mechanism of drought tolerance through stomatal closure by H2O2 generation in guard cells, regulated by a NAC-protein in banana.

Keywords

Musa Banana NAC Drought H2O2 Stomata 

Notes

Acknowledgements

Authors thank, Head, Nuclear Agriculture and Biotechnology Division, BARC for support and encouragement. The work was supported from funding of Department of Atomic Energy, Government of India.

Author contributions

SN, HT, and TG conceived and designed research. SN, and HT conducted the experiments and analyzed data. SN, HT, and TG wrote the manuscript. All authors read and approved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declares that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Sanjana Negi
    • 1
    • 2
  • Himanshu Tak
    • 1
    • 2
  • T. R. Ganapathi
    • 1
  1. 1.Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology DivisionBhabha Atomic Research CentreMumbaiIndia
  2. 2.Homi Bhabha National InstituteMumbaiIndia

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