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Plant Molecular Biology

, Volume 96, Issue 4–5, pp 375–392 | Cite as

The calmodulin-like protein, CML39, is involved in regulating seed development, germination, and fruit development in Arabidopsis

  • Ubaid Midhat
  • Michael K. Y. Ting
  • Howard J. Teresinski
  • Wayne A. Snedden
Article

Abstract

Key message

We show that the calcium sensor, CML39, is important in various developmental processes from seeds to mature plants. This study bridges previous work on CML39 as a stress-induced gene and highlights the importance of calcium signalling in plant development.

Abstract

In addition to the evolutionarily-conserved Ca2+ sensor, calmodulin (CaM), plants possess a large family of CaM-related proteins (CMLs). Using a cml39 loss-of-function mutant, we investigated the roles of CML39 in Arabidopsis and discovered a range of phenotypes across developmental stages and in different tissues. In mature plants, loss of CML39 results in shorter siliques, reduced seed number per silique, and reduced number of ovules per pistil. We also observed changes in seed development, germination, and seed coat properties in cml39 mutants in comparison to wild-type plants. Using radicle emergence as a measure of germination, cml39 mutants showed more rapid germination than wild-type plants. In marked contrast to wild-type seeds, the germination of developing, immature cml39 seeds was not sensitive to cold-stratification. In addition, germination of cml39 seeds was less sensitive than wild-type to inhibition by ABA or by treatments that impaired gibberellic acid biosynthesis. Tetrazolium red staining indicated that the seed-coat permeability of cml39 seeds is greater than that of wild-type seeds. RNA sequencing analysis of cml39 seedlings suggests that changes in chromatin modification may underlie some of the phenotypes associated with cml39 mutants, consistent with previous reports that orthologs of CML39 participate in gene silencing. Aberrant ectopic expression of transcripts for seed storage proteins in 7-day old cml39 seedlings was observed, suggesting mis-regulation of early developmental programs. Collectively, our data support a model where CML39 serves as an important Ca2+ sensor during ovule and seed development, as well as during germination and seedling establishment.

Keywords

Calcium Signal transduction Calmodulin CML Arabidopsis Development 

Notes

Acknowledgements

We are grateful to Profs. Sharon Regan and Jacqueline Monaghan (Queen’s Univ) for helpful discussions. This research was financially supported by Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery, and Research Tool and Infrastructure grants to WAS.

Author contributions

WAS designed and supervised this study. UM and HJT designed and performed the experiments. MKYT contributed to bioinformatic data analysis. All authors contributed to writing, editing, and approval of the article.

Supplementary material

11103_2018_703_MOESM1_ESM.pdf (22.6 mb)
Supplementary material 1 (PDF 23152 KB)
11103_2018_703_MOESM2_ESM.xlsx (225 kb)
Supplementary material 2 (XLSX 225 KB)
11103_2018_703_MOESM3_ESM.pdf (61 kb)
Supplementary material 3 (PDF 60 KB)

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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiologyQueen’s UniversityKingstonCanada

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