Plant Molecular Biology

, Volume 95, Issue 4–5, pp 345–357 | Cite as

OsPPR6, a pentatricopeptide repeat protein involved in editing and splicing chloroplast RNA, is required for chloroplast biogenesis in rice

  • Jianpeng Tang
  • Wenwei Zhang
  • Kai Wen
  • Gaoming Chen
  • Juan Sun
  • Yunlu Tian
  • Weijie Tang
  • Jun Yu
  • Hongzhou An
  • Tingting Wu
  • Fei Kong
  • William Terzaghi
  • Chunming Wang
  • Jianmin Wan
Article

Abstract

Key message

OsPPR6, a pentatricopeptide repeat protein involved in editing and splicing chloroplast RNA, is required for chloroplast biogenesis in rice.

Abstract

The chloroplast has its own genetic material and genetic system, but it is also regulated by nuclear-encoded genes. However, little is known about nuclear-plastid regulatory mechanisms underlying early chloroplast biogenesis in rice. In this study, we isolated and characterized a mutant, osppr6, that showed early chloroplast developmental defects leading to albino leaves and seedling death. We found that the osppr6 mutant failed to form thylakoid membranes. Using map-based cloning and complementation tests, we determined that OsPPR6 encoded a new Pentatricopeptide Repeat (PPR) protein localized in plastids. In the osppr6 mutants, mRNA levels of plastidic genes transcribed by the plastid-encoded RNA polymerase decreased, while those of genes transcribed by the nuclear-encoded RNA polymerase increased. Western blot analyses validated these expression results. We further investigated plastidic RNA editing and splicing in the osppr6 mutants and found that the ndhB transcript was mis-edited and the ycf3 transcript was mis-spliced. Therefore, we demonstrate that OsPPR6, a PPR protein, regulates early chloroplast biogenesis and participates in editing of ndhB and splicing of ycf3 transcripts in rice.

Keywords

Rice PPR Chloroplast biogenesis RNA editing RNA splicing 

Abbreviations

TEM

Transmission electron microscope

NEP

Nuclear-encoded plastid RNA polymerase

PEP

Plastid-encoded RNA polymerase

PPR

Pentatricopeptide repeat protein

MORF

Multiple organellar RNA editing factors

RIP

Receptor interacting protein

EMS

Ethyl methyl sulfonate

ORF

Open reading frame

SSR

Simple sequence repeats

Chl

Chlorophyll

GFP

Green fluorescent protein

CTP

Chloroplast transit peptide

ROS

Reactive oxygen species

DAB

Diaminobenzidine

NBT

Nitroblue tetrazolium

CBB

Stain coomassie brilliant blue stain

RNAi

RNA interference

HEMC

Porphobilinogen deaminase

Notes

Acknowledgements

The National Key Research and Development Project (2016YFD0101107, 2016YFD0100700), and National Key Technology Support Program project (2015BAD01B02-7), China supported this study. CW was supported by the “Shuangchuang”, Project (2012), and “Innovation Team Core Member” (2013) 140, Jiangsu Province, China. The funding agencies had no role in the study design, data collection and analysis, decision to publish, or manuscript preparation.

Author Contributions

CW conceived the experiments and wrote the manuscript; JW supervised this research; J.T. performed the experiments and wrote the manuscript; WZ and FK provided the plant materials; KW, GC, JS, YT, TW, JY, HA and TW participated in the experiments; WT revised manuscript; the all authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

11103_2017_654_MOESM1_ESM.docx (6.5 mb)
Supplementary material 1 (DOCX 6652 KB)

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Jianpeng Tang
    • 1
    • 2
  • Wenwei Zhang
    • 1
  • Kai Wen
    • 1
  • Gaoming Chen
    • 1
  • Juan Sun
    • 1
  • Yunlu Tian
    • 1
  • Weijie Tang
    • 1
  • Jun Yu
    • 1
  • Hongzhou An
    • 1
  • Tingting Wu
    • 1
  • Fei Kong
    • 1
  • William Terzaghi
    • 4
  • Chunming Wang
    • 1
    • 2
  • Jianmin Wan
    • 1
    • 3
  1. 1.State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina
  2. 2.Jiangsu Collaborative Innovation Center for Modern Crop ProductionNanjingChina
  3. 3.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijingChina
  4. 4.Department of BiologyWilkes UniversityWilkes-BarreUSA

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