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Identification and characterization of plant-specific NAC gene family in canola (Brassica napus L.) reveal novel members involved in cell death

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Abstract

NAC transcription factors are plant-specific and play important roles in plant development processes, response to biotic and abiotic cues and hormone signaling. However, to date, little is known about the NAC genes in canola (or oilseed rape, Brassica napus L.). In this study, a total of 60 NAC genes were identified from canola through a systematical analysis and mining of expressed sequence tags. Among these, the cDNA sequences of 41 NAC genes were successfully cloned. The translated protein sequences of canola NAC genes with the NAC genes from representative species were phylogenetically clustered into three major groups and multiple subgroups. The transcriptional activities of these BnaNAC proteins were assayed in yeast. In addition, by quantitative real-time RT-PCR, we further observed that some of these BnaNACs were regulated by different hormone stimuli or abiotic stresses. Interestingly, we successfully identified two novel BnaNACs, BnaNAC19 and BnaNAC82, which could elicit hypersensitive response-like cell death when expressed in Nicotiana benthamiana leaves, which was mediated by accumulation of reactive oxygen species. Overall, our work has laid a solid foundation for further characterization of this important NAC gene family in canola.

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Acknowledgments

This work was supported by a startup funding and in part by the National Natural Science Foundation of China (No. 31471153 to Y-Q. J and 31301648 to B.Y.). We would like to acknowledge assoc. prof. Liu X–Y (NWAFU) for providing the pBS-mCherry vector and, prof. Michael K. Deyholos (University of Alberta, Canada) for providing the canola seeds and critical reading of this manuscript.

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Correspondence to Yuan-Qing Jiang.

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Boya Wang, Xiaohua Guo, Chen Wang and Jieyu Ma have contributed equally to this work.

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Wang, B., Guo, X., Wang, C. et al. Identification and characterization of plant-specific NAC gene family in canola (Brassica napus L.) reveal novel members involved in cell death. Plant Mol Biol 87, 395–411 (2015). https://doi.org/10.1007/s11103-015-0286-1

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