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RETRACTED ARTICLE: Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton

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This article was retracted on 04 September 2018

Abstract

Plant annexins are members of a diverse, multigene protein family that has been associated with a variety of cellular processes and responses to abiotic stresses. GhAnn1, which encodes a putative annexin protein, was isolated from a cotton (Gossypium hirsutum L. acc 7235) cDNA library. Tissue-specific expression showed that GhAnn1 is expressed at differential levels in all tissues examined and strongly induced by various phytohormones and abiotic stress. In vivo and in vitro subcellular localization suggested that GhAnn1 is located in the plasma membrane. In response to drought and salt stress, transgenic cotton plants overexpressing GhAnn1 showed significantly higher germination rates, longer roots, and more vigorous growth than wild-type plants. In addition, plants overexpressing GhAnn1 had higher total chlorophyll content, lower lipid peroxidation levels, increased peroxidase activities, and higher levels of proline and soluble sugars, all of which contributed to increased salt and drought stress tolerance. However, transgenic cotton plants in which the expression of GhAnn1 was suppressed showed the opposite results compared to the overexpressing plants. These findings demonstrated that GhAnn1 plays an important role in the abiotic stress response, and that overexpression of GhAnn1 in transgenic cotton improves salt and drought tolerance.

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Acknowledgments

This program was financially supported in part by National Science Foundation in China (31171590), the National Transgenic Program (2011ZX08005-004), Jiangsu Agriculture Science and Technology Innovation Fund (CX(14)2065), and a project funded by PAPD-JHEI and JCIC-MCP.

Conflict of interest

The authors have declared that no competing interests exist.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, MOE, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People’s Republic of China

    Feng Zhang, Shufen Li, Shuming Yang, Like Wang & Wangzhen Guo

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Correspondence to Wangzhen Guo.

Additional information

The authors are retracting this article because irregularities were involved in the reuse of stomatal images in Figure 9. In details, 1) stomatal image in 0d:S-1 (A) is the same as that in 3As-1 (A); 2) 0d:As-1 (A) was reused in mock As-1 and in mock 3As-1(D); 3) 20d:S-1 (A) and 20d:S-2 (A) were reused in ABA:As-1(D) and ABA:As-2 (D), respectively.

The authors regret any inconvenience or trouble caused by the original publication and apologize sincerely to the readers, reviewers, and editors of Plant Molecular Biology. All authors agree to this retraction.

Electronic supplementary material

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11103_2014_260_MOESM1_ESM.tif

Intron–exon structures of 14 annexin genes from G. raimondii. The scale bar represents 0.2 amino acid substitutions per site. Gene structures were drawn using the online tool Gene Structure Display Server (http://gsds.cbi.pku.edu.cn/). (TIFF 90 kb)

11103_2014_260_MOESM2_ESM.tif

Characterization of transgenic cotton plants. A: PCR analysis of GhAnn1 transgenic plants using NPTII-specific and promoter-gene primers, respectively. M: marker DL2000. P: positive control. WT, wild-type. a - GhAnn1 gene sense vector. b - GhAnn1 gene antisense vector. c - GhAnn1 3′ fragment antisense vector. B: PCR analysis of the NPTII gene. C: Southern blot analysis of T3 transgenic cotton plants for transgene copy number using the restriction enzyme EcoRI, which has a single recognition site in the T-DNA. 1 to 8 - GhAnn1 overexpressing transgenic lines 1 to 8. (TIFF 366 kb)

Supplementary material 3 (DOCX 26 kb)

Supplementary material 4 (DOCX 24 kb)

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Zhang, F., Li, S., Yang, S. et al. RETRACTED ARTICLE: Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton. Plant Mol Biol 87, 47–67 (2015). https://doi.org/10.1007/s11103-014-0260-3

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