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New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus

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Abstract

Apocarotenoid compounds play diverse communication functions in plants, some of them being as hormones, pigments and volatiles. Apocarotenoids are the result of enzymatic cleavage of carotenoids catalyzed by carotenoid cleavage dioxygenase (CCD). The CCD4 family is the largest family of plant CCDs, only present in flowering plants, suggesting a functional diversification associated to the adaptation for specific physiological capacities unique to them. In saffron, two CCD4 genes have been previously isolated from the stigma tissue and related with the generation of specific volatiles involved in the attraction of pollinators. The aim of this study was to identify additional CCD4 members associated with the generation of other carotenoid-derived volatiles during the development of the stigma. The expression of CsCCD4c appears to be restricted to the stigma tissue in saffron and other Crocus species and was correlated with the generation of megastigma-4,6,8-triene. Further, CsCCD4c was up-regulated by wounding, heat, and osmotic stress, suggesting an involvement of its apocarotenoid products in the adaptation of saffron to environmental stresses. The enzymatic activity of CsCCD4c was determined in vivo in Escherichia coli and subsequently in Nicotiana benthamiana by analyzing carotenoids by HPLC–DAD and the volatile products by GC/MS. β-Carotene was shown to be the preferred substrate, being cleaved at the 9,10 (9′,10′) bonds and generating β-ionone, although β-cyclocitral resulting from a 7,8 (7′,8′) cleavage activity was also detected at lower levels. Lutein, neoxanthin and violaxanthin levels in Nicotiana leaves were markedly reduced when CsCCD4c is over expressed, suggesting that CsCCD4c recognizes these carotenoids as substrates.

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Acknowledgments

We thank J. Argandoña (Instituto Botánico, Universidad de Castilla-La Mancha, Albacete, Spain) for excellent technical support, and K. A. Walsh for language revision. The laboratory is supported by the Spanish Ministerio de Ciencia e Innovación (BIO2013-44239-R) and participates in the IBERCAROT network (112RT0445). Dr. Ahrazem was funded by FPCYTA through the INCRECYT Programme.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Departamento de Ciencia y Tecnología Agroforestal y Genética, Facultad de Farmacia, Instituto Botánico, Universidad de Castilla-La Mancha, Campus Universitario s/n, 02071, Albacete, Spain

    Angela Rubio-Moraga, Almudena Trapero-Mozos, Oussama Ahrazem & Lourdes Gómez-Gómez

  2. Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain

    José Luis Rambla, Asun Fernández-de-Carmen, Diego Orzáez & Antonio Granell

  3. Fundación Parque Científico y Tecnológico de Albacete, Albacete, Spain

    Oussama Ahrazem

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  1. Angela Rubio-Moraga
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  2. José Luis Rambla
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  8. Lourdes Gómez-Gómez
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Correspondence to Lourdes Gómez-Gómez.

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Rubio-Moraga, A., Rambla, J.L., Fernández-de-Carmen, A. et al. New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus . Plant Mol Biol 86, 555–569 (2014). https://doi.org/10.1007/s11103-014-0250-5

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