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Plant Molecular Biology

, Volume 86, Issue 4–5, pp 395–407 | Cite as

The pineapple AcMADS1 promoter confers high level expression in tomato and Arabidopsis flowering and fruiting tissues, but AcMADS1 does not complement the tomato LeMADS-RIN (rin) mutant

  • Richard L. Moyle
  • Jonni H. Koia
  • Julia Vrebalov
  • James Giovannoni
  • Jose R. BotellaEmail author
Article

Abstract

A previous EST study identified a MADS box transcription factor coding sequence, AcMADS1, that is strongly induced during non-climacteric pineapple fruit ripening. Phylogenetic analyses place the AcMADS1 protein in the same superclade as LeMADS-RIN, a master regulator of fruit ripening upstream of ethylene in climacteric tomato. LeMADS-RIN has been proposed to be a global ripening regulator shared among climacteric and non-climacteric species, although few functional homologs of LeMADS-RIN have been identified in non-climacteric species. AcMADS1 shares 67 % protein sequence similarity and a similar expression pattern in ripening fruits as LeMADS-RIN. However, in this study AcMADS1 was not able to complement the tomato rin mutant phenotype, indicating AcMADS1 may not be a functionally conserved homolog of LeMADS-RIN or has sufficiently diverged to be unable to act in the context of the tomato network of interacting proteins. The AcMADS1 promoter directed strong expression of the GUS reporter gene to fruits and developing floral organs in tomato and Arabidopsis thaliana, suggesting AcMADS1 may play a role in flower development as well as fruitlet ripening. The AcMADS1 promoter provides a useful molecular tool for directing transgene expression, particularly where up-regulation in developing flowers and fruits is desirable.

Keywords

Ananas comosus Fruit ripening Promoter analysis Beta-glucuronidase MADS box transcription factor 

Notes

Acknowledgments

US-Israel BARD projects IS-4223-09C and IS-4371-10C provided support for this project.

Supplementary material

11103_2014_236_MOESM1_ESM.pptx (465 kb)
Supplementary material Fig. 1 Putative cis-acting elements identified in the AcMADS1 promoter sequence. A signal scan search of the Plant Cis-acting Element Database (PLACE) identified the following motifs: GAGA8HVBKN3 (homeobox domain), MNF1ZMPPC1 (C4 PEPC element), CARGCW8GAT (MADS SEPELLATA AGL15 element), ANAERO2CONSENSUS (anaerobic fermentation related motif), WBOXHVISO1 (sugar response element), ABREMOTIFAOSOSEM (abscisic acid response element), CMSRE1IBSPOA (sugar and sucrose induced motif), ASF1MOTIFCAMV stress-auxin related motif), B2GMAUX28 (auxin response), SP8BFIBSP8BIB (alpha amylase related motif), IBOXCORE (light regulated motif), BIHD1OS (disease resistance element), EECCRCAH1 (CO2-responsive element), SITEIIATCYTC (oxidative phosphorylation related element). The putative TATA box and ATG translation initiation site are boxed. Numbering is relative to the ATG translation initiation site (PPTX 465 kb)
11103_2014_236_MOESM2_ESM.xlsx (19 kb)
Supplementary material Fig. 2 Comparison of cis-acting elements identified in the AcMADS1 and LeMADS-RIN promoter sequences. A signal scan search of the Plant Cis-acting Element Database (PLACE) identified the copy number of known elements present within each promoter sequence (XLSX 18 kb)
11103_2014_236_MOESM3_ESM.docx (16 kb)
Supplementary material Fig. 3 Homology relations of AcMADS1 and other MADS-box proteins. Homology table includes full length sequences of pineapple AcMADS1 (CO7331330.1), tomato MADS-RIN (AF448522.1), pepper MADS-RIN (DQ999998.1), strawberry FvMADS-9 (AF484683.1), tomato TDR5 (X60480.1), tomato MADS1 (AY294329.1), grape API-like (XM_002263374.1), grape MADS2 (AF373601.1), strawberry MADS1-like (GQ398009.1), banana MADS1 (EU869307.1), banana MADS3 (EU869308.1), citrus CiSEP1 (AB329715.1), apple PI (AJ291490.1), Arabidopsis AGL6 (NM_130127.1), peach MADS2 (AAZ16241.1) (DOCX 16 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Richard L. Moyle
    • 1
  • Jonni H. Koia
    • 1
  • Julia Vrebalov
    • 2
  • James Giovannoni
    • 2
  • Jose R. Botella
    • 1
    Email author
  1. 1.Plant Genetic Engineering Laboratory, School of Agriculture and Food SciencesUniversity of QueenslandBrisbaneAustralia
  2. 2.US Department of AgricultureBoyce Thompson Institute for Plant ResearchIthacaUSA

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