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Expression of a gene encoding a rice RING zinc-finger protein, OsRZFP34, enhances stomata opening

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Abstract

By oligo microarray expression profiling, we identified a rice RING zinc-finger protein (RZFP), OsRZFP34, whose gene expression increased with high temperature or abscisic acid (ABA) treatment. As compared with the wild type, rice and Arabidopsis with OsRZFP34 overexpression showed increased relative stomata opening even with ABA treatment. Furthermore, loss-of-function mutation of OsRZFP34 and AtRZFP34 (At5g22920), an OsRZFP34 homolog in Arabidopsis, decreased relative stomata aperture under nonstress control conditions. Expressing OsRZFP34 in atrzfp34 reverted the mutant phenotype to normal, which indicates a conserved molecular function between OsRZFP34 and AtRZFP34. Analysis of water loss and leaf temperature under stress conditions revealed a higher evaporation rate and cooling effect in OsRZFP34-overexpressing Arabidopsis and rice than the wild type, atrzfp34 and osrzfp34. Thus, stomata opening, enhanced leaf cooling, and ABA insensitivity was conserved with OsRZFP34 expression. Transcription profiling of transgenic rice overexpressing OsRZFP34 revealed many genes involved in OsRZFP34-mediated stomatal movement. Several genes upregulated or downregulated in OsRZFP34-overexpressing plants were previously implicated in Ca2+ sensing, K+ regulator, and ABA response. We suggest that OsRZFP34 may modulate these genes to control stomata opening.

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Abbreviations

ABA:

Abscisic acid

Col:

Arabidopsis thaliana ecotype Columbia

HS:

Heat shock

HSPs:

Heat shock proteins

OS:

Opening solution

ROS:

Reactive oxygen species

RZFP:

RING zinc-finger protein

sHSPs:

Small heat shock proteins

TRIM:

Taiwan rice insertion mutant

WT:

Wild type

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Acknowledgments

We are grateful to Drs. Tong-Seung Tseng, Tzung-Meng Wu, and Kuo-Chen Yeh for comments and suggestions on the manuscript; and Dr. Yee-Yung Charng for technical assistance with thermal imaging. We thank the electron microscopy laboratory of Tzu Chi University. We also thank ABRC and TRIM for providing seeds. This work was supported by the National Science Council, Taiwan, ROC (NSC96-2317-B-008-003 and NSC98-2324-B-008-002 to C.H. Yeh).

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Authors and Affiliations

  1. Department of Life Sciences, National Central University, Taoyuan, Taiwan

    Kuo-Hsuan Hsu, Shaw-Jye Wu, Ying-Yu Kuo, Chung-An Lu, Ching-Rong Wu, Pei-Jyun Lian, Yi-Ting Ke, Juin-Hua Huang & Ching-Hui Yeh

  2. Department of Life Sciences, Tzu Chi University, Hualien, Taiwan

    Chia-Chin Liu

  3. Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan

    Chwan-Yang Hong

  4. Institute of Systems Biology and Bioinformatics, National Central University, Taoyuan, Taiwan

    Ching-Hui Yeh

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  1. Kuo-Hsuan Hsu
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Correspondence to Ching-Hui Yeh.

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Hsu, KH., Liu, CC., Wu, SJ. et al. Expression of a gene encoding a rice RING zinc-finger protein, OsRZFP34, enhances stomata opening. Plant Mol Biol 86, 125–137 (2014). https://doi.org/10.1007/s11103-014-0217-6

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