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Plant Molecular Biology

, Volume 86, Issue 1–2, pp 125–137 | Cite as

Expression of a gene encoding a rice RING zinc-finger protein, OsRZFP34, enhances stomata opening

  • Kuo-Hsuan Hsu
  • Chia-Chin Liu
  • Shaw-Jye Wu
  • Ying-Yu Kuo
  • Chung-An Lu
  • Ching-Rong Wu
  • Pei-Jyun Lian
  • Chwan-Yang Hong
  • Yi-Ting Ke
  • Juin-Hua Huang
  • Ching-Hui YehEmail author
Article

Abstract

By oligo microarray expression profiling, we identified a rice RING zinc-finger protein (RZFP), OsRZFP34, whose gene expression increased with high temperature or abscisic acid (ABA) treatment. As compared with the wild type, rice and Arabidopsis with OsRZFP34 overexpression showed increased relative stomata opening even with ABA treatment. Furthermore, loss-of-function mutation of OsRZFP34 and AtRZFP34 (At5g22920), an OsRZFP34 homolog in Arabidopsis, decreased relative stomata aperture under nonstress control conditions. Expressing OsRZFP34 in atrzfp34 reverted the mutant phenotype to normal, which indicates a conserved molecular function between OsRZFP34 and AtRZFP34. Analysis of water loss and leaf temperature under stress conditions revealed a higher evaporation rate and cooling effect in OsRZFP34-overexpressing Arabidopsis and rice than the wild type, atrzfp34 and osrzfp34. Thus, stomata opening, enhanced leaf cooling, and ABA insensitivity was conserved with OsRZFP34 expression. Transcription profiling of transgenic rice overexpressing OsRZFP34 revealed many genes involved in OsRZFP34-mediated stomatal movement. Several genes upregulated or downregulated in OsRZFP34-overexpressing plants were previously implicated in Ca2+ sensing, K+ regulator, and ABA response. We suggest that OsRZFP34 may modulate these genes to control stomata opening.

Keywords

ABA Heat stress Rice RING zinc finger protein Stomata Transpiration 

Abbreviations

ABA

Abscisic acid

Col

Arabidopsis thaliana ecotype Columbia

HS

Heat shock

HSPs

Heat shock proteins

OS

Opening solution

ROS

Reactive oxygen species

RZFP

RING zinc-finger protein

sHSPs

Small heat shock proteins

TRIM

Taiwan rice insertion mutant

WT

Wild type

Notes

Acknowledgments

We are grateful to Drs. Tong-Seung Tseng, Tzung-Meng Wu, and Kuo-Chen Yeh for comments and suggestions on the manuscript; and Dr. Yee-Yung Charng for technical assistance with thermal imaging. We thank the electron microscopy laboratory of Tzu Chi University. We also thank ABRC and TRIM for providing seeds. This work was supported by the National Science Council, Taiwan, ROC (NSC96-2317-B-008-003 and NSC98-2324-B-008-002 to C.H. Yeh).

Supplementary material

11103_2014_217_MOESM1_ESM.pdf (46 kb)
Supplementary material 1 (PDF 45 kb)
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Supplementary material 2 (PDF 63 kb)
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Supplementary material 3 (PDF 53 kb)
11103_2014_217_MOESM4_ESM.pdf (236 kb)
Supplementary material 4 (PDF 235 kb)
11103_2014_217_MOESM5_ESM.docx (11 kb)
Supplementary material 5 (DOCX 11 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Kuo-Hsuan Hsu
    • 1
  • Chia-Chin Liu
    • 2
  • Shaw-Jye Wu
    • 1
  • Ying-Yu Kuo
    • 1
  • Chung-An Lu
    • 1
  • Ching-Rong Wu
    • 1
  • Pei-Jyun Lian
    • 1
  • Chwan-Yang Hong
    • 3
  • Yi-Ting Ke
    • 1
  • Juin-Hua Huang
    • 1
  • Ching-Hui Yeh
    • 1
    • 4
    Email author
  1. 1.Department of Life SciencesNational Central UniversityTaoyuanTaiwan
  2. 2.Department of Life SciencesTzu Chi UniversityHualienTaiwan
  3. 3.Department of Agricultural ChemistryNational Taiwan UniversityTaipeiTaiwan
  4. 4.Institute of Systems Biology and BioinformaticsNational Central UniversityTaoyuanTaiwan

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