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Plant Molecular Biology

, Volume 84, Issue 3, pp 359–369 | Cite as

Novel role of ZmaNAC36 in co-expression of starch synthetic genes in maize endosperm

  • Junjie Zhang
  • Jiang Chen
  • Qiang Yi
  • Yufeng Hu
  • Hanmei Liu
  • Yinghong Liu
  • Yubi HuangEmail author
Article

Abstract

Starch is an essential commodity that is widely used as food, feed, fuel and in industry. However, its mechanism of synthesis is not fully understood, especially in terms of the expression and regulation of the starch synthetic genes. It was reported that the starch synthetic genes were co-expressed during maize endosperm development; however, the mechanism of the co-expression was not reported. In this paper, the ZmaNAC36 gene was amplified by homology-based cloning, and its expression vector was constructed for transient expression. The nuclear localization, transcriptional activation and target sites of the ZmaNAC36 protein were identified. The expression profile of ZmaNAC36 showed that it was strongly expressed in the maize endosperm and was co-expressed with most of the starch synthetic genes. Moreover, the expressions of many starch synthesis genes in the endosperm were upregulated when ZmaNAC36 was transiently overexpressed. All our results indicated that NAC36 might be a transcription factor and play a potential role in the co-expression of starch synthetic genes in the maize endosperm.

Keywords

Starch synthesis Gene regulation Transcription factor NAC Maize endosperm 

Abbreviations

AGPase

ADP-glucose pyrophosphorylase

ADPG

Adenosine diphosphate glucose

ATAF1/2

Arabidopsis thaliana transcription activation factor

CUC2

Cup-shaped cotyledon

DAP

Day after pollination

DBE

Debranching enzymes

GBSS

Granule-bound starch synthase

IPTG

Isopropyl β-d-1-thiogalactopyranoside

SBE

Starch-branching enzymes

SSS

Soluble granule-bound starch synthase

SP

Starch phosphorylase

Notes

Acknowledgments

This work was supported by the National Key Basic Research Program of China (No: 2014CB138205), cultivating fund of excellent master degree theses of Sichuan Agriculture University, and the Preferentially Financing projects of scientific and technological activities of overseas students in Sichuan province.

Supplementary material

11103_2013_153_MOESM1_ESM.doc (284 kb)
Supplementary material 1 (DOC 284 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Junjie Zhang
    • 1
    • 2
  • Jiang Chen
    • 1
  • Qiang Yi
    • 1
  • Yufeng Hu
    • 3
  • Hanmei Liu
    • 2
  • Yinghong Liu
    • 1
  • Yubi Huang
    • 1
    • 3
    Email author
  1. 1.Maize Research InstituteSichuan Agricultural UniversityChengduChina
  2. 2.College of Life ScienceSichuan Agricultural UniversityYa’anChina
  3. 3.College of AgricultureSichuan Agricultural UniversityChengduChina

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