Novel role of ZmaNAC36 in co-expression of starch synthetic genes in maize endosperm
- 1k Downloads
Starch is an essential commodity that is widely used as food, feed, fuel and in industry. However, its mechanism of synthesis is not fully understood, especially in terms of the expression and regulation of the starch synthetic genes. It was reported that the starch synthetic genes were co-expressed during maize endosperm development; however, the mechanism of the co-expression was not reported. In this paper, the ZmaNAC36 gene was amplified by homology-based cloning, and its expression vector was constructed for transient expression. The nuclear localization, transcriptional activation and target sites of the ZmaNAC36 protein were identified. The expression profile of ZmaNAC36 showed that it was strongly expressed in the maize endosperm and was co-expressed with most of the starch synthetic genes. Moreover, the expressions of many starch synthesis genes in the endosperm were upregulated when ZmaNAC36 was transiently overexpressed. All our results indicated that NAC36 might be a transcription factor and play a potential role in the co-expression of starch synthetic genes in the maize endosperm.
KeywordsStarch synthesis Gene regulation Transcription factor NAC Maize endosperm
Adenosine diphosphate glucose
Arabidopsis thaliana transcription activation factor
Day after pollination
Granule-bound starch synthase
Soluble granule-bound starch synthase
This work was supported by the National Key Basic Research Program of China (No: 2014CB138205), cultivating fund of excellent master degree theses of Sichuan Agriculture University, and the Preferentially Financing projects of scientific and technological activities of overseas students in Sichuan province.
- Ferreira S, Senning M, Sonnewald S, Keßling PM, Goldstein R, Sonnewald U (2010) Comparative transcriptome analysis coupled to X-ray CT reveals sucrose supply and growth velocity as major determinants of potato tuber starch biosynthesis. BMC Genomics 11:93–110PubMedCentralPubMedCrossRefGoogle Scholar
- Hannah LC (2005) Starch synthesis in the maize endosperm. Maydica 50:497–506Google Scholar
- Hennen-Bierwagen TA, Lin Q, Grimaud F, Planchot V, Keeling PL, James MG, Myers AM (2009) Proteins from multiple metabolic pathways associate with starch biosynthetic enzymes in high molecular weight complexes: a model for regulation of carbon allocation in maize amyloplasts. Plant Physiol 149(3):1541–1559PubMedCentralPubMedCrossRefGoogle Scholar
- Keeling PL (1999) From enzyme activity to flux control: a quest to understand starch deposition in developing cereal grains. In: Bryant JA, Burrell MM, Kruger NJ (eds) Plant carbohydrate biochemistry. BIOS Scientific Publishers, Oxford, pp 91–103Google Scholar
- Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York, pp 1486–1493 (Chinese version)Google Scholar
- Zeeman SC, Thorneycroft D, Schupp N, Chapple A, Weck M, Dunstan H, Haldimann P, Bechtold N, Smith AM, Smith SM (2004) Plastidial α-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress. Plant Physiol 135(2):849–858PubMedCentralPubMedCrossRefGoogle Scholar