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Splice variants of the SIP1 transcripts play a role in nodule organogenesis in Lotus japonicus

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Abstract

SymRK-interacting protein 1 (SIP1) has previously been shown to interact with the symbiosis receptor kinase, SymRK, in Lotus japonicus. A longer variant of the SIP1 transcript, SIP1L, was isolated and characterized. SIP1L contains an additional 17 amino acids that make its C-terminus a complete heat shock protein 20 (Hsp20)-like domain. In contrast to SIP1S, the longer splicing variant SIP1L could not interact with SymRK. Both SIP1L and SIP1S transcripts could be detected in developing nodules and other plant tissues, although the former was always more abundant than the latter. SIP1L and SIP1S formed heteromeric protein complexes, which were co-localized in the plasma membrane, cytoplasm and nuclei. Expression of SIP1-RNAi in transgenic hairy roots resulted in impairment in the nodule and arbuscular mycorrhizal development, suggesting an important role of SIP1 in the common symbiosis pathway. Overexpression of either SIP1L or SIP1S increased the number of nodules formed on transgenic hairy roots, indicating a positive role of SIP1 in nodulation. The SIP1S-like transcript was not detected in other higher plants tested, and the SIP1L-like proteins of these plants were capable of interacting with the SymRK orthologs. It is proposed that the loss of the ability of SIP1L to interact with SymRK in Lotus is compensated by the expression of a shorter splicing variant, SIP1S, which binds SymRK and may play a role in relaying the symbiosis signals to downstream cellular events.

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Acknowledgments

This work was supported by the National Basic Research Program of China (973 Program grant no. 2010CB126502), the National Natural Science Foundation of China (grant no. 31170224, 30900096), the Ministry of Agriculture of China (grant no. 2009ZX08009-116B), the Fok Ying-Tong Education Foundation, China (grant no. 122038), the Fundamental Research Funds for the Central Universities (grant no. 2011PY136), the Huazhong Agricultural University Scientific & Technological Self-innovation Foundation (grant no.2011SC02) and the China National Fundamental Fund of Personnel Training (Grant no. J1103510).

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  1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China

    Chao Wang, Hui Zhu, Liping Jin, Tao Chen, Longxiang Wang, Heng Kang & Zhongming Zhang

  2. Department of Plant, Soil and Entomological Sciences and Program of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID, 84844, USA

    Zonglie Hong

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  1. Chao Wang
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Chao Wang and Hui Zhu contributed equally to this work.

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Wang, C., Zhu, H., Jin, L. et al. Splice variants of the SIP1 transcripts play a role in nodule organogenesis in Lotus japonicus . Plant Mol Biol 82, 97–111 (2013). https://doi.org/10.1007/s11103-013-0042-3

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