Abstract
Majerová et al. (Plant Mol Biol, 2011) have recently reported that a considerable fraction of cytosines at tobacco telomeres is methylated. Although the data presented in this report indicate that tobacco telomeric sequences undergo certain levels of DNA methylation, it is not clear whether the methylated sequences are at telomeres, at internal chromosomal loci or at both.
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Cokus S, Feng S, Zhang X, Chen Z, Merriman B, Haudenschild C, Pradhan S, Nelson S, Pellegrini M, Jacobsen S (2008) Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning. Nature 452:215–219
Fajkus J, Kovarík A, Královics R, Bezdek M (1995a) Organization of telomeric and subtelomeric chromatin in the higher plant Nicotiana tabacum. Mol Gen Genet 247:633–638
Fajkus J, Královics R, Kovaríc A, Fajkusová L (1995b) The telomeric sequence is directly attached to the HRS60 subtelomeric tandem repeat in tobacco chromosomes. FEBS Lett 364:33–35
Gámez-Arjona F, López-López C, Vaquero-Sedas M, Vega-Palas M (2010) On the organization of the nucleosomes associated with telomeric sequences. Biochim Biophys Acta 1803:1058–1061
Horáková M, Fajkus J (2000) TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes. Genome 43:273–284
Kenton A, Parokonny A, Gleba Y, Bennett M (1993) Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics. Mol Gen Genet 240:159–169
Majerová E, Fojtová M, Mozgová I, Bittová M, Fajkus J (2011) Hypomethylating drugs efficiently decrease cytosine methylation in telomeric DNA and activate telomerase without affecting telomere lengths in tobacco cells. Plant Mol Biol. doi:10.1007/s11103-011-9816-7
Meyne J et al (1990) Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes. Chromosoma 99:3–10
Regad F, Lebas M, Lescure B (1994) ITSs within the Arabidopsis thaliana genome. J Mol Biol 239:163–169
Richards E, Ausubel F (1988) Isolation of a higher eukaryotic telomere from Arabidopsis thaliana. Cell 53:127–136
Richards E, Goodman H, Ausubel F (1991) The centromere region of Arabidopsis thaliana chromosome 1 contains telomere-similar sequences. Nucleic Acids Res 19:3351–3357
Suzuki K, Yamagiwa Y, Matsui T, Yoshida K (1994) Restriction enzyme-resistant high molecular weight telomeric DNA fragments in tobacco. DNA Res 1:129–138
Uchida W, Matsunaga S, Sugiyama R, Kawano S (2002) Interstitial telomere-like repeats in the Arabidopsis thaliana genome. Genes Genet Syst 77:63–67
Vaquero-Sedas M, Vega-Palas M (2011) On the chromatin structure of eukaryotic telomeres. Epigenetics 6:1055–1058
Vaquero-Sedas M, Gámez-Arjona F, Vega-Palas M (2010) Arabidopsis telomeres exhibit euchromatic features. Nucleic Acids Res. doi:10.1093/nar/gkq1119
Vershinin A, Heslop-Harrison J (1998) Comparative analysis of the nucleosomal structure of rye, wheat and their relatives. Plant Mol Biol 36:149–161
Vrbsky J, Akimcheva S, Watson J, Turner T, Daxinger L, Vyskot B, Aufsatz W, Riha K (2010) siRNA-mediated methylation of Arabidopsis telomeres. PLoS Genet 6:e1000986
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Vaquero-Sedas, M.I., Vega-Palas, M.A. DNA methylation at tobacco telomeric sequences. Plant Mol Biol 77, 529–531 (2011). https://doi.org/10.1007/s11103-011-9833-6
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DOI: https://doi.org/10.1007/s11103-011-9833-6