Plant Molecular Biology

, 77:355

Identification of metabolites related to mechanisms of resistance in barley against Fusarium graminearum, based on mass spectrometry

  • Venkatesh Bollina
  • Ajjamada C. Kushalappa
  • Thin M. Choo
  • Yves Dion
  • Sylvie Rioux
Article

DOI: 10.1007/s11103-011-9815-8

Cite this article as:
Bollina, V., Kushalappa, A.C., Choo, T.M. et al. Plant Mol Biol (2011) 77: 355. doi:10.1007/s11103-011-9815-8

Abstract

Fusarium head blight (FHB) is an economically important disease of the family Triticeae, as, apart from yield reduction it also causes quality deterioration by producing mycotoxins. Host resistance is the most promising way to control the disease. Metabolic profiling was applied to identify resistance related (RR) metabolites against Fusarium graminearum in five FHB-resistant genotypes (‘Chevron’, ‘H5277-44’, ‘H5277-164’, ‘M92-513’ and ‘M122’) relative to one FHB-susceptible genotype (‘Stander’). The disease severity was assessed in greenhouse to group the genotypes based on FHB-resistance. The disease was quantified as the proportion of diseased spikelets (PSD) and the area under the disease progress curve (AUDPC). Spikelets were collected at 72 h post inoculation. Metabolites were extracted into an aqueous solution of methanol and analyzed using a LC-hybrid-MS system. Metabolite abundances were subjected to a resistant versus susceptible pair-wise analysis, using a t test. Resistance related (RR) metabolites, both constitutive (RRC) and induced (RRI), were identified amongst metabolites whose levels were significantly higher in resistant genotype than in susceptible. Among 1,430 RR metabolites, 115 were putatively identified. These RR metabolites belonged to different chemical groups: fatty acids: linolenic acid; phenylpropanoids: p-coumaric, sinapic acid; flavonoids: naringenin, kaempferol glucoside, catechol glucoside. In addition, resistance indicator metabolites, such as deoxynivalenol (DON) and DON-3-O-glucoside (D3G) were also detected. The amount of total DON synthesized converted to D3G (PDC) was the greatest in resistant genotype ‘Chevron’ (PDC = 0.76). The role of the resistance-related and resistance-indicator metabolites on plant defense, and their use as potential biomarkers to screen barley genotypes for FHB resistance is discussed.

Keywords

Fusarium graminearum Gibberella zeae Fusarium head blight Metabolomics Biomarker Disease resistance Hordeum vulgare 

Abbreviations

AME

Accurate mass error

AUDPC

Area under disease progress curve

CAN

Canonical vector

CDA

Canonical discriminant analysis

D3G

DON-3-O-glucoside

DON

Deoxynivalenol

FHB

Fusarium head blight

GC-MS

Gas chromatography mass spectrometry

JA

Jasmonic acid

LC-MS

Liquid chromatography mass spectrometry

PDC

Proportion of DON converted to D3G

PR

Pathogenesis related

PSD

Proportion of diseased spikelets

QTL

Quantitative trait loci

RM

Resistant mock-inoculated

RP

Resistant pathogen-inoculated

RR

Resistance-related

RRC

Resistance-related constitutive

RRI

Resistance-related induced

SM

Susceptible mock-inoculated

SP

Susceptible pathogen-inoculated

TDP

Total DON produced

Supplementary material

11103_2011_9815_MOESM1_ESM.doc (296 kb)
Supplementary material 1 (DOC 295 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Venkatesh Bollina
    • 1
  • Ajjamada C. Kushalappa
    • 1
  • Thin M. Choo
    • 2
  • Yves Dion
    • 3
  • Sylvie Rioux
    • 4
  1. 1.Plant Science DepartmentMcGill UniversitySainte-Anne-de-BellevueCanada
  2. 2.Eastern Cereal and Oilseed Research CentreAgriculture and Agri-Food CanadaOttawaCanada
  3. 3.Centre de Recherche Sur Les Grains Inc.Saint-Mathieu-de-BeloeilCanada
  4. 4.Centre de Recherche Sur Les Grains Inc.Ste. FoyCanada

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