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Seed-expressed casein kinase I acts as a positive regulator of the SeFAD2 promoter via phosphorylation of the SebHLH transcription factor

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Abstract

Microsomal oleic acid desaturase (FAD2) catalyzes the first committed step of the biosynthesis of polyunsaturated fatty acids via extra-plastidial desaturation of oleic acid to linoleic acid. In the regulatory mechanism controlling seed-specific SeFAD2 expression, trans-activation of the seed-specific SeFAD2 promoter is mediated by the SebHLH transcription factor (Kim et al. in Plant Mol Biol 64:453–466, 2007). In this study, a protein interacting with SebHLH was isolated from yeast two-hybrid analysis. The protein shares approximately 80% sequence identity with other putative casein kinases and was named SeCKI (Sesame Casein Kinase I). SeCKI transcripts were predominantly expressed in developing sesame seeds and were induced approximately threefold by exogenous application of ABA. eGFP:SeCKI fusion protein was localized to the nucleus. The SeCKI protein specifically bound to SebHLH. The SeCKI protein was autophosphorylated in a calcium-independent manner and transphosphorylated the SebHLH protein. Both the SebHLH and the SeCKI genes or both the SebHLH and mutated SemCKI (K182G) genes, under the control of CaMV 35S promoter, and the GUS reporter gene driven by SeFAD2 promoter containing E- and G-Box motifs were co-expressed in developing sesame seeds. This co-expression revealed that SeCKI enhanced the SebHLH-mediated transactivation of the SeFAD2 gene promoter via phosphorylation of the SebHLH transcription factor.

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Acknowledgments

This work was supported by grants from 15 Agenda Program (PJ00744108), the Rural Development Administration, Republic of Korea, and Basic Research Program (20090064298) and the World Class University Project (R31-2009-000-20025-0), National Research Foundation of Korea. We also acknowledge the Korea Basic Science Institute, Gwangju Center, for the confocal microscopy and image analysis.

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Authors and Affiliations

  1. School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea

    Mi Jung Kim, Youn Sung Kim & Jeong Sheop Shin

  2. Department of Bioenergy Science and Technology, Chonnam National University, Gwangju, 500-757, Republic of Korea

    Young Sam Go, Saet Buyl Lee & Mi Chung Suh

  3. Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, 790-784, Korea

    Myung Ki Min & Inhwan Hwang

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  1. Mi Jung Kim
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Correspondence to Mi Chung Suh.

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The nucleotide sequence of the SeCKI cDNA from Sesamum indicum has been registered in the GenBank™/EBI Data Bank under accession number GQ444335.

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Kim, M.J., Go, Y.S., Lee, S.B. et al. Seed-expressed casein kinase I acts as a positive regulator of the SeFAD2 promoter via phosphorylation of the SebHLH transcription factor. Plant Mol Biol 73, 425–437 (2010). https://doi.org/10.1007/s11103-010-9630-7

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