Abstract
Self-incompatibility (SI) in Lolium perenne is controlled gametophytically by the S–Z two-locus system. S and Z loci mapped to L. perenne linkage groups 1 and 2, respectively, with their corresponding putative-syntenic regions on rice chromosome 5 (R5) and R4. None of the gene products of S and Z have yet been identified. SI cDNA libraries were developed to enrich for SI expressed genes in L. perenne. Transcripts were identified from the SI libraries that were orthologous to sequences on rice R4 and R5. These represent potential SI candidate genes. Altogether ten expressed SI candidate genes were identified. A rapid increase in gene expression within two minutes after pollen-stigma contact was revealed, reaching a maximum between 2 and 10 min. The potential involvement of these genes in the SI reactions is discussed.
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Abbreviations
- CBL:
-
Calcineurin B-like
- GO:
-
Gene ontology
- GSI:
-
Gametophytic self-incompatibility
- ILGI:
-
International lolium genome initiative
- LG:
-
Linkage group
- MAPK:
-
Mitogen-activated protein kinase
- PCD:
-
Programmed cell death
- PR:
-
Pathogenesis-related
- SCR:
-
S-Locus cysteine-rich protein
- SCP:
-
Sperm-coating glycoprotein
- SI:
-
Self-incompatibility
- SLF:
-
S-Locus F-box protein
- SRK:
-
S-Locus receptor protein kinase
- SSH:
-
Suppression subtractive hybridization
- SSI:
-
Sporophytic self-incompatibility
- STS:
-
Sequence tagged site
- TDF:
-
Transcribed derived fragment
- UBP:
-
Ubiquitin-specific protease
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Acknowledgments
BY was funded by a Teagasc Walsh Fellowship. We acknowledge core funding contributions of Teagasc and financial contributions of the National Development Plan.
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Accession Number Sequence data from this article can be found in the EMBL/GenBank data libraries under accession numbers AM991118, AM991119, AM991120, AM991121, AM991122, AM991123, AM991124, AM991125, AM991126 and AM991127.
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Yang, B., Thorogood, D., Armstead, I.P. et al. Identification of genes expressed during the self-incompatibility response in perennial ryegrass (Lolium perenne L.). Plant Mol Biol 70, 709–723 (2009). https://doi.org/10.1007/s11103-009-9501-2
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DOI: https://doi.org/10.1007/s11103-009-9501-2