Conserved and novel miRNAs in the legume Phaseolus vulgaris in response to stress

Arenas-Huertero, Catalina; Pérez, Beatriz; Rabanal, Fernando; Blanco-Melo, Daniel; De la Rosa, Carlos; Estrada-Navarrete, Georgina; Sanchez, Federico; Covarrubias, Alejandra Alicia; Reyes, José Luis

doi:10.1007/s11103-009-9480-3

Published: 08 April 2009

Conserved and novel miRNAs in the legume Phaseolus vulgaris in response to stress

Catalina Arenas-Huertero¹,
Beatriz Pérez¹,
Fernando Rabanal¹,
Daniel Blanco-Melo¹,
Carlos De la Rosa¹,
Georgina Estrada-Navarrete¹,
Federico Sanchez¹,
Alejandra Alicia Covarrubias¹ &
José Luis Reyes¹

Plant Molecular Biology volume 70, pages 385–401 (2009)Cite this article

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Abstract

MicroRNAs (miRNAs) are small RNA molecules recognized as important regulators of gene expression. Although plant miRNAs have been extensively studied in model systems, less is known in other plants with limited genome sequence data. We are interested in the identification of miRNAs in Phaseolus vulgaris (common bean) to uncover different plant strategies to cope with adverse conditions and because of its relevance as a crop in developing countries. Here we present the identification of conserved and candidate novel miRNAs in P. vulgaris present in different organs and growth conditions, including drought, abscisic acid treatment, and Rhizobium infection. We also identified cDNA sequences in public databases that represent the corresponding miRNA precursors. In addition, we predicted and validated target mRNAs amongst reported EST and cDNAs for P. vulgaris. We propose that the novel miRNAs present in common bean and other legumes, are involved in regulation of legume-specific processes including adaptation to diverse external cues.

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Acknowledgments

We thank the help of Jerome Verleyen and Roberto Rodríguez during the early phases of sequence analysis. This work was supported by grants from CONACyT, Mexico (J48740) and DGAPA-UNAM (IN-227706) to JLR, and JLR and AAC, respectively.

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Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, 62210, Cuernavaca, Morelos, Mexico
Catalina Arenas-Huertero, Beatriz Pérez, Fernando Rabanal, Daniel Blanco-Melo, Carlos De la Rosa, Georgina Estrada-Navarrete, Federico Sanchez, Alejandra Alicia Covarrubias & José Luis Reyes

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Catalina Arenas-Huertero
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Beatriz Pérez
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Fernando Rabanal
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Daniel Blanco-Melo
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Carlos De la Rosa
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Georgina Estrada-Navarrete
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Federico Sanchez
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Alejandra Alicia Covarrubias
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José Luis Reyes
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Correspondence to José Luis Reyes.

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Pre-miRNAs for conserved P. vulgaris miRNAs (XLS 24 kb)

Predicted miRNA targets for conserved miRNAs in P. vulgaris (XLS 48 kb)

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Arenas-Huertero, C., Pérez, B., Rabanal, F. et al. Conserved and novel miRNAs in the legume Phaseolus vulgaris in response to stress. Plant Mol Biol 70, 385–401 (2009). https://doi.org/10.1007/s11103-009-9480-3

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Received: 31 October 2008
Accepted: 05 March 2009
Published: 08 April 2009
Issue Date: July 2009
DOI: https://doi.org/10.1007/s11103-009-9480-3

Keywords

miRNAs
Water deficit
Glycine max
Medicago truncatula
Common bean
Abscisic acid