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Reversed end Ds element: a novel tool for chromosome engineering in Arabidopsis

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Abstract

The maize Ac/Ds transposable element (TE) transposes by a “cut and paste” mechanism. Previous studies in maize showed that when the TE ends are in reversed orientation with respect to each other, alternative transposition reactions can occur resulting in large scale genome rearrangements including deletions and inversions. To test whether similar genome rearrangements can also occur in other plants, we studied the efficacy of such alternative transposition-mediated genome rearrangements in Arabidopsis. Here we present our analysis of 33 independent chromosome rearrangements. Transposition at the reversed ends Ds element can cause deletions over 1 Mbp, and inversions up to 2.4 Mbp in size. We identified additional rearrangements including a reciprocal translocation and a putative ring chromosome. Some of the deletions and inversions are germinally transmitted.

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Acknowledgements

We thank R. Martienssen for plasmid pAJ6, D. Voytas and D. Wright for plasmid pDW418, and C. Waddell for Arabidopsis plants carrying rbcS:Ac-1017. Lisa Coffey, Tanya Rogers, Peter Howe, Avni Sanghi and Michelle Blessington assisted with various aspects of this study. This research was supported by NSF Award 0110170 to T.P.

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  1. Lakshminarasimhan Krishnaswamy

    Present address: Division of Biological Sciences, Tucker Hall, University of Missouri, Columbia, MO, USA

Authors and Affiliations

  1. Department of Genetics, Development and Cell Biology, Iowa State University, 2208 Molecular Biology Building, Ames, IA, 50011-3260, USA

    Lakshminarasimhan Krishnaswamy, Jianbo Zhang & Thomas Peterson

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  1. Lakshminarasimhan Krishnaswamy
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Correspondence to Thomas Peterson.

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Krishnaswamy, L., Zhang, J. & Peterson, T. Reversed end Ds element: a novel tool for chromosome engineering in Arabidopsis. Plant Mol Biol 68, 399–411 (2008). https://doi.org/10.1007/s11103-008-9377-6

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