Complexity of Hsp90 in organelle targeting

Abstract

Heat shock protein 90 (Hsp90) is an abundant and highly conserved molecular chaperone. In Arabidopsis, the Hsp90 gene family consists of seven members. Here, we report that the AtHsp90-6 gene gives rise to two mRNA populations, termed AtHsp90-6L and AtHsp90-6S due to alternative initiation of transcription. The AtHsp90-6L and AtHsp90-6S transcription start sites are located 228 nucleotides upstream and 124 nucleotides downstream of the annotated translation start site, respectively. Both transcripts are detected under normal or heat-shock conditions. The inducibility of AtHsp90-6 mRNAs by heat shock implies a potential role of both isoforms in stress management. Stable transformation experiments with fusion constructs between the N-terminal part of each AtHsp90-6 isoform and green fluorescent protein indicated import of both fusion proteins into mitochondria. In planta investigation confirmed that fusion of the AtHsp90-5 N-terminus to green fluorescent protein (GFP) did result in specific chloroplastic localization. The mechanisms of regulation for mitochondria- and plastid-localized chaperone-encoding genes are not well understood. Future work is needed to address the possible roles of harsh environmental conditions and developmental processes on fine-tuning and compartmentalization of the AtHsp90-6L, AtHsp90-6S, and AtHsp90-5 proteins in Arabidopsis.

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Acknowledgements

We would like to thank Elli Hatzistavrou for technical assistance. This work was partly supported by a grant to PH from the GSRT, Greece (PENED 01/148) and Pythagoras I.

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Correspondence to Polydefkis Hatzopoulos.

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Prassinos, C., Haralampidis, K., Milioni, D. et al. Complexity of Hsp90 in organelle targeting. Plant Mol Biol 67, 323–334 (2008). https://doi.org/10.1007/s11103-008-9322-8

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Keywords

  • Alternative transcription
  • Chloroplasts
  • Heat shock
  • Hsp90
  • Mitochondria
  • Targeting