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The FT/TFL1 gene family in grapevine

Plant Molecular Biology volume 63, pages 637–650 (2007)Cite this article

Abstract

The FT/TFL1 gene family encodes proteins with similarity to phosphatidylethanolamine binding proteins which function as flowering promoters and repressors. We show here that the FT/TFL1 gene family in Vitis vinifera is composed of at least five genes. Sequence comparisons with homologous genes identified in other dicot species group them in three major clades, the FT, MFT and TFL1 subfamilies, the latter including three of the Vitis sequences. Gene expression patterns are in agreement with a role of VvFT and VvMFT as flowering promoters; while VvTFL1A, VvTFL1B and VvTFL1C could be associated with vegetative development and maintenance of meristem indetermination. Overexpression of VvFT in transgenic Arabidopsis plants generates early flowering phenotypes similar to those produced by FT supporting a role for this gene in flowering promotion. Overexpression of VvTFL1A does not affect flowering time but the determination of flower meristems, strongly altering inflorescence structure, which is consistent with the biological roles assigned to similar genes in other species.

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Abbreviations

SAM:

shoot apical meristem

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Acknowledgments

We thank Félix Cabello and the Instituto Madrileño de Investigación y Desarrollo Rural Agrario y Alimentario (IMIDRA, Alcalá de Henares, Madrid) for providing plant materials for this research, Gemma Bravo for her excellent technical assistance with the transformation experiments, and Diego Lijavetsky for his help with the MEGA software. We also thank Cheo Machín for careful editing of the manuscript. This research was supported by grants GEN2003-2023-CO2-01 and BIO2005-07612-C02-01 from Spanish Ministerio de Educación y Ciencía and GRAPEGEN from a collaborative agreement between Genoma España and Genome Canada.

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Authors and Affiliations

  1. Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040, Madrid, Spain

    María José Carmona & Myriam Calonje

  2. Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Darwin 3, Cantoblanco, 28049, Madrid, Spain

    José Miguel Martínez-Zapater

  3. Department of Plant and Microbial Biology, University of California, Berkeley, California , 94720, USA

    Myriam Calonje

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  1. María José Carmona
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Correspondence to José Miguel Martínez-Zapater.

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Carmona, M.J., Calonje, M. & Martínez-Zapater, J.M. The FT/TFL1 gene family in grapevine. Plant Mol Biol 63, 637–650 (2007). https://doi.org/10.1007/s11103-006-9113-z

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  • DOI: https://doi.org/10.1007/s11103-006-9113-z

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