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Plant Molecular Biology

, Volume 62, Issue 4–5, pp 655–668 | Cite as

Molecular dissection of the interaction between the transcriptional activator ZmMRP-1 and the promoter of BETL-1

  • Cristina Barrero
  • Luis M. Muñiz
  • Elisa Gómez
  • Gregorio Hueros
  • Joaquín RoyoEmail author
Article

Abstract

The interaction between the transfer cell specific transcriptional activator ZmMRP-1 and the promoter of the transfer cell specific gene BETL-1 constitutes an exceptionally robust system. Reporter constructs containing the BETL-1 promoter are virtually silent in a variety of cell types, from maize leaves to yeast. The introduction of ZmMRP-1 in co-transformation assays leads to the transactivation of the reporter construct by up to two orders of magnitude. In this work we have investigated the molecular basis of this interaction. We found that the BETL-1 promoter includes four potential targets for ZmMRP-1 binding, consisting of a 12 bp motif containing two repeats. Co-transformation assays and electrophoretic mobility shift experiments identified the sequence TATCTCTATCTC as the preferred one for the interaction with the transcription factor. Identification of similar sequences in other transfer cell specific promoters lead us to propose as a transfer cell box a sequence related to those identified in the BETL-1 promoter, positioned 50–100 bp upstream the TATA box.

Keywords

Endosperm transfer cells Maize Transcriptional regulation 

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Notes

Acknowledgements

This work was supported by grants to G.H: BIO2000-0848 and BIO2003-03721 from the Spanish MCYT. We thank Yolanda Sanz for providing technical support. J.R. is supported by a ``Ramón y Cajal'' contract of the Spanish MCYT.

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Cristina Barrero
    • 1
  • Luis M. Muñiz
    • 1
  • Elisa Gómez
    • 1
  • Gregorio Hueros
    • 1
  • Joaquín Royo
    • 1
    Email author
  1. 1.Departamento de Biología Celular y GenéticaUniversidad de AlcaláAlcala de Henares (Madrid)Spain

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