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Identification and functional expression of the pepper pathogen-induced gene, CAPIP2, involved in disease resistance and drought and salt stress tolerance

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Abstract

A novel pathogen-induced gene, designated CAPIP2, was isolated from pepper leaves infected with Xanthomonas campestris pv. vesicatoria. CAPIP2:GFP fusion proteins were primarily localized in the cytoplasm. The CAPIP2 transcripts were constitutively expressed in the pepper leaves, flowers, and fruits, but were not detected in the stems and roots. CAPIP2 gene expression was induced strongly in the pepper leaves during pathogen infection, and also after exposure to abiotic elicitors and environmental stresses. Ectopic CAPIP2 expression in Arabidopsis was accompanied by the expression of Arabidopsis PR-1 and PDF1.2 genes. Overexpression of the CAPIP2 gene in Arabidopsis transgenic plants conferred enhanced resistance to Pseudomonas syringae pv. tomato DC3000. The CAPIP2 transgenic Arabidopsis also manifested increased tolerance to high salt, drought and oxidative stress during seed germination and seedling state. These results suggest that pepper CAPIP2 gene may function as a defense-related gene against both biotic and abiotic stresses.

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Acknowledgements

This work was financially supported by a grant (CG1432) from the Crop Functional Genomics Center of the 21st Century Frontier Research Program, and also by a grant from the Center for Plant Molecular Genetics and Breeding Research, Seoul National University in Korea. We thank Michael Rabba for reading the manuscript.

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  1. Sang Hee Kim

    Present address: Department of Plant Microbiology and Pathology, Division of Plant Sciences, The University of Missouri, Columbia, MO65211, USA

Authors and Affiliations

  1. Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University, Seoul, 136-713, Republic of Korea

    Sung Chul Lee, Sang Hee Kim, Soo Hyun An & Byung Kook Hwang

  2. National Instrumentation Center for Environmental Management, Seoul National University, Seoul, 151-921, Republic of Korea

    Seung Youn Yi

  3. Laboratory of Molecular Plant Pathology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-713, Republic of Korea

    Byung Kook Hwang

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  1. Sung Chul Lee
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Correspondence to Byung Kook Hwang.

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Lee, S.C., Kim, S.H., An, S.H. et al. Identification and functional expression of the pepper pathogen-induced gene, CAPIP2, involved in disease resistance and drought and salt stress tolerance. Plant Mol Biol 62, 151–164 (2006). https://doi.org/10.1007/s11103-006-9010-5

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