Plant Molecular Biology

, Volume 62, Issue 1–2, pp 99–110 | Cite as

ASK1, a SKP1 homolog, is required for nuclear reorganization, presynaptic homolog juxtaposition and the proper distribution of cohesin during meiosis in Arabidopsis

  • Dazhong Zhao
  • Xiaohui Yang
  • Li Quan
  • Ljudmilla Timofejeva
  • Nathan W. Rigel
  • Hong MaEmail author
  • Christopher A. MakaroffEmail author


Nuclear reorganization and juxtaposition of homologous chromosomes at late leptotene/early zygotene are essential steps before chromosome synapsis at pachytene. We report the results of detailed studies, which demonstrate that nuclear reorganization and homolog juxtapositioning processes are defective in a null mutant, ask1-1. Our results from 4, 6-diamino-2-phenylindole (DAPI)-stained spreads showed that the “synizetic knot”, which is typically found in wild type (WT) meiosis during late leptotene and zygotene, was missing in the ask1-1 mutant. Furthermore, ask1-1 meiocytes exhibited only limited homolog juxtaposition at centromere regions at early zygotene. Immunodetection of the cohesin protein SYN1 identified ask1 defects in cohesin distribution from zygotene to anaphase I. Analysis of meiotic chromosomes in ask1-1 and syn1 single mutants, as well as an ask1-1 syn1 double mutant indicate that ASK1 is required for normal SYN1 distribution during meiotic prophase I and suggest that ask1 associated defects may be primarily related to SYN1 mislocalization.


Arabidopsis thaliana ASK1 Homolog juxtaposition Meiosis SYN1 synizetic knot Cohesin Synapsis 



axial element


central element


4, 6-diamino-2-phenylindole


lateral elements


synaptonemal complex


transmission electron microscopy


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We thank A. Omeis and J. Wang for plant care. We also thank E. Harris and M. Henry for their help with the identification of the ask1-1 syn1 double mutant plants. We are grateful for comments on this manuscript from C. Chen, W. Li, L. Harper, W. Ni, G. Wang, L.M. Zahn, W. Zhang, and M. Zolan. This work was supported by grants from the National Science Foundation [MCB-0092075 (HM); MCB-0322171 (CAM)], the National Institutes of Health [RO1 GM63871 (HM)], and the Department of Energy [DE-FG02-02ER15332 (HM and DZ)] and by funds from the Department of Biology and the Huck Institutes of the Life Sciences at the Pennsylvania State University. H.M. gratefully acknowledges the support of the John Simon Guggenheim Memorial Foundation.


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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Dazhong Zhao
    • 1
    • 3
  • Xiaohui Yang
    • 2
  • Li Quan
    • 1
  • Ljudmilla Timofejeva
    • 1
    • 4
  • Nathan W. Rigel
    • 1
  • Hong Ma
    • 1
    Email author
  • Christopher A. Makaroff
    • 2
    Email author
  1. 1.Department of Biology and the Huck Institutes of the Life SciencesThe Pennsylvania State UniversityUniversity ParkUSA
  2. 2.Department of Chemistry and BiochemistryMiami UniversityOxfordUSA
  3. 3.Department of Biological SciencesUniversity of Wisconsin-MilwaukeeMilwaukeeUSA
  4. 4.Department of Gene TechnologyTallinn University of TechnologyTallinnEstonia

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