Disease Resistance in Plants that Carry a Feedback-regulated Yeast Poly(A) Binding Protein Gene
It has been reported that the expression of the yeast poly(A) binding protein gene (PAB1) in plants leads to an induction of disease resistance responses, accompanied by alterations in the growth habit of the plant (Li et al. Plant Mol. Biol. (2000) 42 335). To capitalize on this observation, a feedback-regulated PAB1 gene was assembled and introduced into tobacco and Arabidopsis. The regulation entailed the linking of the expression of the PAB1 gene to control by the lac repressor, and by linking lac repressor expression to the disease resistance state of the plant, such that the induction of systemic defense responses by accumulation of the yeast poly(A) binding protein would turn off the expression of the PAB1 gene. Plants containing this system showed elevated and/or constitutive expression of disease-associated genes and significant resistance to otherwise pathogenic organisms. As well, they displayed a nearly normal growth habit under laboratory and greenhouse settings. These studies indicate that the expression of cytotoxic genes (such as the PAB1 gene) in plants can be controlled so that enhanced disease resistance can be achieved without significantly affecting plant growth and development.
Keywordsconstitutive PR gene expression disease resistance feedback regulation lac repressor yeast poly(A) binding protein
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- Gallagher, S.R. 1992
Quantitation of GUS activity by fluorometryGallagher, SR eds. GUS Protocols: Using the GUS Gene as a Reporter of Gene ExpressionAcademic PressNew York4759Google Scholar
- Gorlach, J., Volrath, S., Knauf-Beiter, G., Hengy, G., Beckhove, U., Kogel, K.H., Oostendorp, M., Staub, T., Ward, E., Kessmann, H., Ryals, J. 1996Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheatPlant Cell8629643PubMedCrossRefGoogle Scholar
- Morris, S.W., Vernooij, B., Titatarn, S., Starrett, M., Thomas, S., Wiltse, C.C., Frederiksen, R.A., Bhandhufalck, A., Hulbert, S., Uknes, S. 1998Induced resistance responses in maizeMol. Plant Microb. Interact.11643658Google Scholar
- Oh, J.-W., Kong, Q., Song, C., Carpenter, C.D., Simon, A.E. 1995Open reading frames of turnip crinkle virus involved in satellite symptom expression and incompatibility with Arabidopsis thaliana ecotype DijonMol. Plant Microb. Interact.8979987Google Scholar
- Petersen, M., Brodersen, P., Naested, H., Andreasson, E., Lindhart, U., Johansen, B., Nielsen, H.B., Lacy, M., Austin, M.J., Parker, J.E., Sharma, S.B., Klessig, D.F., Martienssen, R., Mattsson, O., Jensen, A.B., Mundy, J. 2000Arabidopsis MAP kinase 4 negatively regulates systemic acquired resistanceCell10311111120PubMedCrossRefGoogle Scholar
- Reuveni, M., Siegel, M.R., Nesmith, W.C. 1985Bioassays using detached tobacco leaves to determine the sensitivity of Peronospora tabacina to fungicidesPesticide Sci.16244249Google Scholar
- Rufty, R.C., Main, C.E. 1989Components of partial resistance to blue mold in six tobacco genotypes under controlled environmental conditionsPhytopathol79606609Google Scholar
- Thomma, B., Eggermont, K., Penninckx, I., Mauch-Mani, B., Vogelsang, R., Cammue, B.P.A, Broekaert, W.F. 1998Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogensProc. Natl. Acad. Sci. USA951510715111CrossRefGoogle Scholar
- Xing, H., Lawrence, C.B., Chambers, O., Davies, H.M., Everett, N.P., and Li, Q.Q. 2006. Increased pathogen resistance and yield in transgenic plants expressing combinations of the modified antimicrobial peptides based on indolicidin and magainin. Planta, in press (advanced publication online; DOI 10.1007/s00425–005–0143–6)Google Scholar