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Stable Plastid Transformation in Lettuce (Lactuca sativa L.)

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Abstract

Although plastid transformation in higher plants was first demonstrated in the early 1990s it is only recently that the technology is being extended to a broader range of species. To date, the production of fertile transplastomic plants has been reported for tobacco, tomato, petunia, soybean, cotton and Lesquerella fendleri (Brassicaceae). In this study we demonstrate a polyethylene glycol-mediated plastid transformation system for lettuce that generates fertile, homoplasmic, plastid-transformed lines. Transformation was achieved using a vector that targets genes to the trnA/trnI intergenic region of the lettuce plastid genome employing the aadA gene as a selectable marker against spectinomycin. Spectinomycin resistance and heterologous gene transcription were shown in T1 plants derived from self-pollinated primary regenerants demonstrating transmission of the plastid-encoded transgene to the first seed generation. Crossing with male sterile wild-type lettuce showed that spectinomycin resistance was not transmitted via pollen. Constructs containing the gfp gene showed plastid-based expression of green fluorescent protein. The lettuce plastid could have potential both as a production and a delivery system for edible human therapeutic proteins.

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Authors and Affiliations

  1. Rijk Zwaan Breeding B.V., 1e Kruisweg 9, 4793 RS, Fijnaart, The Netherlands

    Cilia L. C. Lelivelt, C. Bastiaan deSnoo & Kees M. P. van Dun

  2. Institute of Bioengineering and Agroecology, National University of Ireland, Maynooth, Co. Kildare, Ireland

    Matthew S. McCabe & Jacqueline M. Nugent

  3. Department of Plant Sciences, University of Cambridge, Downing Street, CB2 3EA, Cambridge, UK

    Christine A. Newell, Ian Birch-Machin & John C. Gray

  4. Department of Biochemistry, Trinity College, Dublin 2, Ireland

    Kingston H. G. Mills

  5. Department of Anatomy, University of Cambridge, Downing Street, CB2 3DY, Cambridge, UK

    Ian Birch-Machin

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  1. Cilia L. C. Lelivelt
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  2. Matthew S. McCabe
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  3. Christine A. Newell
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  4. C. Bastiaan deSnoo
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  6. Ian Birch-Machin
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  7. John C. Gray
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  8. Kingston H. G. Mills
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  9. Jacqueline M. Nugent
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Correspondence to Jacqueline M. Nugent.

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Cilia L.C. Lelivelt, Matthew S. McCabe and Ian Birch-Machin - These two authors contributed equally to the project.

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Lelivelt, C.L.C., McCabe, M.S., Newell, C.A. et al. Stable Plastid Transformation in Lettuce (Lactuca sativa L.). Plant Mol Biol 58, 763–774 (2005). https://doi.org/10.1007/s11103-005-7704-8

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