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In vitro and in planta Interaction Evidence between Nicotiana tabacum Thaumatin-Like Protein 1 (TLP1) and Cucumber mosaic virus Proteins

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Abstract

Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (TLP), and were designated as Nicotiana tabacum thaumatin-like protein 1 (NtTLP1). Consistent with this observation, recombinant GST-NtTLP1 protein, which was expressed and purified in E. coli, bound tightly to CMV 1a in vitro. In planta interaction was also verified via co-immunoprecipitation. Additionally, NtTLP1 specifically interacted with the CMV movement-related proteins, movement protein and coat protein, in yeast. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of NtTLP1 increased as the result of CMV inoculation.

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Abbreviations

NtTLP1 :

Nicotiana tabacum thaumatin-like protein 1

CMV:

Cucumber mosaic virus

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  1. School of Life Sciences and Biotechnology, Korea University, 1, 5-ga, Anam-dong, 136-701, Seoul, Sungbuk-gu, Republic of Korea

    Min Jung Kim, Byung-Kook Ham, Hwa Ran Kim, In-Ju Lee, Young Jin Kim, Young In Park & Kyung-Hee Paek

  2. Division of Environmental & Life Sciences, Seoul Women’s University, 139-774, Seoul, Republic of Korea

    Ki Hyun Ryu

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  1. Min Jung Kim
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  2. Byung-Kook Ham
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  3. Hwa Ran Kim
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Correspondence to Kyung-Hee Paek.

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Nucleotide Sequence Database under the GenBank accession number AY745249.

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Kim, M.J., Ham, BK., Kim, H.R. et al. In vitro and in planta Interaction Evidence between Nicotiana tabacum Thaumatin-Like Protein 1 (TLP1) and Cucumber mosaic virus Proteins. Plant Mol Biol 59, 981–994 (2005). https://doi.org/10.1007/s11103-005-2619-y

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