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Arabidopsis Rad51B is important for double-strand DNA breaks repair in somatic cells

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Abstract

Rad51 paralogs belong to the Rad52 epistasis group of proteins and are involved in homologous recombination (HR), especially the assembly and stabilization of Rad51, which is a homolog of RecA in eukaryotes. We previously cloned and characterized two RAD51 paralogous genes in Arabidopsis, named AtRAD51C and AtXRCC3, which are considered the counterparts of human RAD51C and XRCC3, respectively. Here we describe the identification of RAD51B homologue in Arabidopsis, AtRAD51B. We found a higher expression of AtRAD51B in flower buds and roots. Expression of AtRAD51B was induced by genotoxic stresses such as ionizing irradiation and treatment with a cross-linking reagent, cisplatin. Yeast two-hybrid analysis showed that AtRad51B interacted with AtRad51C. We also found and characterized T-DNA insertion mutant lines. The mutant lines were devoid of AtRAD51B expression, viable and fertile. The mutants were moderately sensitive to γ-ray and hypersensitive to cisplatin. Our results suggest that AtRAD51B gene product is involved in the repair of double-strand DNA breaks (DSBs) via HR

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Abbreviations

RACE:

rapid amplification of cDNA ends

RT-PCR:

reverse transcriptase polymerase chain reaction

X-gal:

5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside

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Authors and Affiliations

  1. Department of Plant Biotechnology, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

    Keishi Osakabe, Kiyomi Abe, Hiroaki Ichikawa & Seiichi Toki

  2. Institute of Radiation Breeding, National Institute of Agrobiological Sciences, 2425 Uemurata, Hitachiohmiya, Ibaraki, 319-2293, Japan

    Hiroaki Yamanouchi, Toshio Takyuu, Terutaka Yoshioka & Yuji Ito

  3. Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba, 292-0818, Japan

    Tomohiko Kato & Satoshi Tabata

  4. Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan

    Shunsuke Kurei, Yasushi Yoshioka & Yasunori Machida

  5. RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan

    Motoaki Seki & Kazuo Shinozaki

  6. Experimental Plant Division, RIKEN BioResource Center, Tsukuba, Ibaraki, 305-0074, Japan

    Masatomo Kobayashi

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  1. Keishi Osakabe
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  2. Kiyomi Abe
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  3. Hiroaki Yamanouchi
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  15. Hiroaki Ichikawa
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  16. Seiichi Toki
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Corresponding author

Correspondence to Seiichi Toki.

Additional information

Accession numbers: AB194809 (AtRAD51Bα), AB194810 (AtRAD51Bβ), AB194811 (AtRAD51D).

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Osakabe, K., Abe, K., Yamanouchi, H. et al. Arabidopsis Rad51B is important for double-strand DNA breaks repair in somatic cells. Plant Mol Biol 57, 819–833 (2005). https://doi.org/10.1007/s11103-005-2187-1

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