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A strawberry fruit-specific and ripening-related gene codes for a HyPRP protein involved in polyphenol anchoring

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Abstract

A strawberry (Fragaria x ananassa cv. Chandler) fruit cDNA (Fahyprp-cDNA) and its corresponding gene (Fahyprp) showing sequence homology to higher plant hyprp genes have been isolated. The cDNA contains an open reading frame encoding a 16 kDa protein with 156 amino acids. The peptide has an aminoterminal signal sequence, a repetitive proline-rich sequence, and a cysteine-rich carboxy-terminal region homologous to other HyPRP proteins. Northern blot and QRT-PCR analysis have shown that the strawberry transcript is speci.cally expressed in fruit, not being detected in other plant tissues. “In situ” hybridization and immunolocalization studies have indicated that the Fahyprp gene is strongly expressed in achene sclerenchyma cells, in the vascular and receptacle cells of immature green fruit and in the vascular cells of mature red fruits. The achenes removal from unripe green fruits induced the expression of this Fahyprp gene. This induction was reverted by treatment of deachened fruit with the auxin NAA, supporting the idea that Fahyprp gene expression is regulated by auxins. Furthermore, the HyPRP protein has been localized in parenchymatic cells of immature fruits associated to structures containing condensed tannins. The results are discussed supporting a putative role of this protein in the anchoring of polymeric polyphenols in the strawberry fruit during growth and ripening.

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Abbreviations

CAD:

cinnamyl alcohol deshydrogenase

Ct:

threshold cycle

DMACA:

p-dimethylaminocinnamaldehyde

FaHyprp:

strawberry hybrid proline-rich protein

GRPs:

glycine rich proteins

HyPRPs:

hybrid proline-rich proteins

KLM:

keyhole limpet hemocyanine

NAA:

1-naphthaleneacetic acid

pfu:

plaque formation units

pI:

isoelectric point

PRPs:

proline-rich proteins

QRT-PCR:

quantitative real time PCR

RER:

rough endoplasme reticulum

RT-PCR:

retrotranscriptase polymerase chain reaction

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Authors and Affiliations

  1. Dpto. de Bioquímica y Biología Molecular e Instituto Andaluz de Biotecnología Edificio Severo Ochoa, Universidad de Córdoba, Campus Universitario de Rabanales, 14071, Córdoba, Spain

    Rosario Blanco-Portales, Juan A. López-Raéz, Mari Luz Bellido, Enriqueta Moyano, Gabriel Dorado, Jose L. Caballero & Juan Muñoz-Blanco

  2. Dpto. de Biología Celular, Fisiología e Inmunología, Edificio Severo Ochoa, Universidad de Córdoba, Campus Universitario de Rabanales, 14071, Córdoba, Spain

    José A. González-Reyes

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  1. Rosario Blanco-Portales
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  2. Juan A. López-Raéz
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  3. Mari Luz Bellido
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  4. Enriqueta Moyano
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  5. Gabriel Dorado
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  6. José A. González-Reyes
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  7. Jose L. Caballero
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  8. Juan Muñoz-Blanco
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Corresponding author

Correspondence to Juan Muñoz-Blanco.

Additional information

The nucleotide sequence data reported corresponds to the accession number AF 026382 (cDNA) and AY530533 (genomic DNA).

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Blanco-Portales, R., López-Raéz, J.A., Bellido, M.L. et al. A strawberry fruit-specific and ripening-related gene codes for a HyPRP protein involved in polyphenol anchoring. Plant Mol Biol 55, 763–780 (2004). https://doi.org/10.1007/s11103-005-1966-z

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  • DOI: https://doi.org/10.1007/s11103-005-1966-z

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