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Plant Molecular Biology

, Volume 57, Issue 4, pp 593–612 | Cite as

Gene expression analysis by cDNA-AFLP highlights a set of new signaling networks and translational control during seed dormancy breaking in Nicotiana plumbaginifolia

  • Jérôme Bove
  • Philippe Lucas
  • Béatrice Godin
  • Laurent Ogé
  • Marc Jullien
  • Philippe GrappinEmail author
Article

Abstract

Seed dormancy in Nicotiana plumbaginifolia is characterized by an abscisic acid accumulation linked to a pronounced germination delay. Dormancy can be released by 1 year after-ripening treatment. Using a cDNA-amplified fragment length polymorphism (cDNA-AFLP) approach we compared the gene expression patterns of dormant and after-ripened seedS., air-dry or during one day imbibition and analyzed 15,000 cDNA fragments. Among theM., 1020 were found to be differentially regulated by dormancy. Of 412 sequenced cDNA fragmentS., 83 were assigned to a known function by search similarities to public databases. The functional categories of the identified dormancy maintenance and breaking responsive geneS., give evidence that after-ripening turns in the air-dry seed to a new developmental program that modulateS., at the RNA level, components of translational control, signaling networkS., transcriptional control and regulated proteolysis.

Keywords

Abscisic acid cDNA-AFLP Nicotiana plumbaginifolia Seed dormancy Seed germination 

Abbreviations

ABA

Abscisic acid

cDNA-AFLP

cDNA-amplified fragment length polymorphism

D

Dormant

GA

Gibberellin

ND

Non-dormant

TDF

Transcript derived fragment

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Copyright information

© Springer 2005

Authors and Affiliations

  • Jérôme Bove
    • 1
    • 2
    • 3
  • Philippe Lucas
    • 1
    • 2
  • Béatrice Godin
    • 1
    • 2
  • Laurent Ogé
    • 1
    • 2
  • Marc Jullien
    • 1
    • 2
  • Philippe Grappin
    • 1
    • 2
    Email author
  1. 1.UMR 204 de Biologie des SemencesInstitut National de la Recherche Agronomique, Centre de Versailles-GrignonVersailles CedexFrance
  2. 2.Institut National Agronomique de Paris-GrignonParis cedex 05France
  3. 3.Department of BiologyPennsylvania State UniversityUniversity ParkUSA

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